The overall goal of the Analytical Genomics and Transgenics Core (AGTC) is to enhance the productivity of the UAB Rheumatic Disease Core Center (RDCC) researchers, and provide state-of-the-art services to facilitate the development and use of appropriate genetic animal models. During the two previous funding cycles, this Core (formerly called the "Gene Targeting Core Facility" (GTCF)) served to support expertise in embryonic stem (ES) cell services as part of the UAB Transgenic Mouse Facility. In response to user needs, the Core has expanded services to more specifically assist with the creation of mouse models relevant to rheumatic disease beyond just ES services to 1) generate novel genetically engineered models of broad utility to multiple RDCC investigators, and 2) establish educational and outreach programs to forge active collaborations between the Core and RDCC investigators, especially in areas related to genomics. Formal educational resources for learning modern and emerging genetic and genomic technologies via workshops, seminars, lectures, and symposia hosted at UAB and our partner institution, Hudson Alpha Institute for Biotechnology (HAIB) are an extension of the core's evolution. The overarching objective and downstream output of the Core remains the same;to produce mouse models of human disease and of human genetic variants contributing to disease in order to provide a mammalian system to study the pathophysiology of rheumatic disease, as well as to test the efficacy of potential treatment interventions. To this end, the Analytical Genomics &Transgenic Core has the following specific aims:
AIM 1. SERVICE: To provide expert services to generate and analyze genetic/genomic data, and to develop translational animal models relating to the mission of the RDCC.
AIM 2. OUTREACH AND EDUCATION: To provide enrichment programs for RDCC investigators.
AIM 3. DEVELOPMENT: To assess RDCC investigator needs and develop new platfonns and technologies to address those needs.
Genetically modified mouse models are critical to the understanding ofthe role of genes, identified genetic variants in humans producing disease, and ultimately creating translational models for developing novel therapeutics. The Analytical Genomics and Transgenics Core (AGTC) has been established to address these needs as they relate to the study of rheumatic disease.
|Karki, Suman; Surolia, Ranu; Hock, Thomas David et al. (2014) Wilms' tumor 1 (Wt1) regulates pleural mesothelial cell plasticity and transition into myofibroblasts in idiopathic pulmonary fibrosis. FASEB J 28:1122-31|
|Bullard, Daniel C; Hu, Xianzhen; Crawford, David et al. (2014) Expression of a single ICAM-1 isoform on T cells is sufficient for development of experimental autoimmune encephalomyelitis. Eur J Immunol 44:1194-9|
|Honasoge, Avinash; Shelton, Katherine A; Sontheimer, Harald (2014) Autocrine regulation of glioma cell proliferation via pHe-sensitive K(+) channels. Am J Physiol Cell Physiol 306:C493-505|
|Bowers, Nathan L; Helton, E Scott; Huijbregts, Richard P H et al. (2014) Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway. PLoS Pathog 10:e1003993|
|Londoño-Joshi, Angelina I; Arend, Rebecca C; Aristizabal, Laura et al. (2014) Effect of niclosamide on basal-like breast cancers. Mol Cancer Ther 13:800-11|
|Ballesteros-Tato, André; León, Beatriz; Lee, Byung O et al. (2014) Epitope-specific regulation of memory programming by differential duration of antigen presentation to influenza-specific CD8(+) T cells. Immunity 41:127-40|
|Gray, G Kenneth; McFarland, Braden C; Rowse, Amber L et al. (2014) Therapeutic CK2 inhibition attenuates diverse prosurvival signaling cascades and decreases cell viability in human breast cancer cells. Oncotarget 5:6484-96|
|Fenutría, Rafael; Martinez, Vanesa G; Simões, Inês et al. (2014) Transgenic expression of soluble human CD5 enhances experimentally-induced autoimmune and anti-tumoral immune responses. PLoS One 9:e84895|
|León, Beatriz; Bradley, John E; Lund, Frances E et al. (2014) FoxP3+ regulatory T cells promote influenza-specific Tfh responses by controlling IL-2 availability. Nat Commun 5:3495|
|Liu, Yudong; Holdbrooks, Andrew T; De Sarno, Patrizia et al. (2014) Therapeutic efficacy of suppressing the Jak/STAT pathway in multiple models of experimental autoimmune encephalomyelitis. J Immunol 192:59-72|
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