The Comprehensive Flow Cytometry Core (CFCC) is directed by Dr. John D. Mountz with assistance of Co-Directors, Drs.Troy Randall and Olaf Kutsch. The goal of the CFCC is to enhance the productivity of the research base of the UAB Rheumatic Disease Core Center (RDCC) through state-of-the-art flow cytometry and cell separation technologies. To accomplish this, the Core provides the equipment, service and expertise necessary for the application of flow cytometry and related technologies to cell analyses and cell purification at a reasonable cost. These services play a key role in studies of disease pathogenesis and identification of potential therapeutic targets, as well as in analysis of determinants of disease susceptibility and drug responsiveness, and pre-clinical testing of potential therapeutic reagents.
Our Specific Aims are: 1 Service. To improve service by continued improvements of our equipment, through enhanced sophistication of our user base, optimal efficiency of sample analysis, rigorous quality control of all operations and maintenance of operator proficiency for technologically challenging applications. 2. Outreach &Education. To provide informal tutorials, formal courses, symposia, and web-based information with the goals of increasing our user base through enhanced awareness of flow cytometry and introducing established users to newer technologies and applications. 3. Development. To develop new applications in response to users'needs and to take full advantage of equipment capabilities, through discussions with users, participation in international flow cytometry meetings, and inclusion of knowledgeable core users on our Advisory Committee. To keep pace with research needs of the RDCC investigators, we have expanded the capacity of the CFCC and introduced new equipment and technologies. Continued development of innovative applications is enhanced by the depth of expertise at UAB and external collaborations. Education is accomplished through bi-weekly Individualized Design of Experiments &Analyses Sessions (IDEAs) in which the Director/Co-Directors interact with investigators to develop protocols and applications, including integration of flow cytometry aspects in the experimental design with other Cores, including AIIC and AGTC.
The Comprehensive Flow Cytometry Core provides instrumentation and expertise to support fundamental mechanistic studies of rheumatic diseases and autoimmunity. Furthermore, the CFCC resources support the identification of new biomarkers for disease diagnosis and the development of novel treatments.
|Karki, Suman; Surolia, Ranu; Hock, Thomas David et al. (2014) Wilms' tumor 1 (Wt1) regulates pleural mesothelial cell plasticity and transition into myofibroblasts in idiopathic pulmonary fibrosis. FASEB J 28:1122-31|
|Bullard, Daniel C; Hu, Xianzhen; Crawford, David et al. (2014) Expression of a single ICAM-1 isoform on T cells is sufficient for development of experimental autoimmune encephalomyelitis. Eur J Immunol 44:1194-9|
|Honasoge, Avinash; Shelton, Katherine A; Sontheimer, Harald (2014) Autocrine regulation of glioma cell proliferation via pHe-sensitive K(+) channels. Am J Physiol Cell Physiol 306:C493-505|
|Bowers, Nathan L; Helton, E Scott; Huijbregts, Richard P H et al. (2014) Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway. PLoS Pathog 10:e1003993|
|Londoño-Joshi, Angelina I; Arend, Rebecca C; Aristizabal, Laura et al. (2014) Effect of niclosamide on basal-like breast cancers. Mol Cancer Ther 13:800-11|
|Ballesteros-Tato, André; León, Beatriz; Lee, Byung O et al. (2014) Epitope-specific regulation of memory programming by differential duration of antigen presentation to influenza-specific CD8(+) T cells. Immunity 41:127-40|
|Gray, G Kenneth; McFarland, Braden C; Rowse, Amber L et al. (2014) Therapeutic CK2 inhibition attenuates diverse prosurvival signaling cascades and decreases cell viability in human breast cancer cells. Oncotarget 5:6484-96|
|Fenutría, Rafael; Martinez, Vanesa G; Simões, Inês et al. (2014) Transgenic expression of soluble human CD5 enhances experimentally-induced autoimmune and anti-tumoral immune responses. PLoS One 9:e84895|
|León, Beatriz; Bradley, John E; Lund, Frances E et al. (2014) FoxP3+ regulatory T cells promote influenza-specific Tfh responses by controlling IL-2 availability. Nat Commun 5:3495|
|Liu, Yudong; Holdbrooks, Andrew T; De Sarno, Patrizia et al. (2014) Therapeutic efficacy of suppressing the Jak/STAT pathway in multiple models of experimental autoimmune encephalomyelitis. J Immunol 192:59-72|
Showing the most recent 10 out of 117 publications