The Protein Core facility is one of the research cores in the Rheumatic Diseases Core Center (RDCC) at Washington University. It proposes the following Specific Aims to sustain and develop new state-of-the-art protein services to RDCC members: 1) Support continued progress and growth of the Hybridoma Center;and 2) Support continued progress and growth of the Protein Production and Purification Facility. The Hybridoma Center aids RDCC investigators in the generation of primary hybridomas secreting monoclonal antibodies that are critical to contemporary immunology and rheumatic diseases research. New services are being tested to offer RDCC investigators rabbit monoclonal antibodies and genetic immunization, for example. The Protein Production and Purification Facility aids RDCC investigators by producing and purifying large quantities of monoclonal antibodies for their studies. In addition, it has recently gained expertise in producing and purifying recombinant and native proteins from a number of different expression systems. This service will now be made available to the RDCC community.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR048335-13
Application #
8528471
Study Section
Special Emphasis Panel (ZAR1-MLB)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
13
Fiscal Year
2013
Total Cost
$173,641
Indirect Cost
$59,404
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Yan, Huimin; Zhou, Hui-Fang; Akk, Antonina et al. (2016) Neutrophil Proteases Promote Experimental Abdominal Aortic Aneurysm via Extracellular Trap Release and Plasmacytoid Dendritic Cell Activation. Arterioscler Thromb Vasc Biol 36:1660-9
Chou, Chun; Verbaro, Daniel J; Tonc, Elena et al. (2016) The Transcription Factor AP4 Mediates Resolution of Chronic Viral Infection through Amplification of Germinal Center B Cell Responses. Immunity 45:570-82
Gorman, Matthew J; Poddar, Subhajit; Farzan, Michael et al. (2016) The Interferon-Stimulated Gene Ifitm3 Restricts West Nile Virus Infection and Pathogenesis. J Virol 90:8212-25
Reese, Tiffany A; Bi, Kevin; Kambal, Amal et al. (2016) Sequential Infection with Common Pathogens Promotes Human-like Immune Gene Expression and Altered Vaccine Response. Cell Host Microbe 19:713-9
Park, Sunmin; Buck, Michael D; Desai, Chandni et al. (2016) Autophagy Genes Enhance Murine Gammaherpesvirus 68 Reactivation from Latency by Preventing Virus-Induced Systemic Inflammation. Cell Host Microbe 19:91-101
Chen, Edwin; Salinas, Nichole D; Huang, Yining et al. (2016) Broadly neutralizing epitopes in the Plasmodium vivax vaccine candidate Duffy Binding Protein. Proc Natl Acad Sci U S A 113:6277-82
O'Brien, Valerie P; Hannan, Thomas J; Yu, Lu et al. (2016) A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease. Nat Microbiol 2:16196
Wagner, Erin K; Raychaudhuri, Soumya; Villalonga, Mercedes B et al. (2016) Mapping rare, deleterious mutations in Factor H: Association with early onset, drusen burden, and lower antigenic levels in familial AMD. Sci Rep 6:31531
Poddar, Subhajit; Hyde, Jennifer L; Gorman, Matthew J et al. (2016) The Interferon-Stimulated Gene IFITM3 Restricts Infection and Pathogenesis of Arthritogenic and Encephalitic Alphaviruses. J Virol 90:8780-94
Lyzogubov, Valeriy V; Bora, Puran S; Wu, Xiaobo et al. (2016) The Complement Regulatory Protein CD46 Deficient Mouse Spontaneously Develops Dry-Type Age-Related Macular Degeneration-Like Phenotype. Am J Pathol 186:2088-104

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