Abstract

The role of this Core is to provide proteomics and mass spectrometry support for the investigators in the UAB Skin Diseases Research Center. This will be principally in the form of high-resolution separation of proteins in complex biological samples, coupled with mass spectrometry identification of proteins that comprise the biological samples generated by the various projects. Existing instrumentation m the UAB Comprehensive Cancer Center Mass Spectrometry and Proteomics Shared Facility will be used for the Skin Proteomics Core. Because this resource is heavily utilized, funding from the SDRC will enable investigators to obtain the technical help and expertise for their skin related projects. The Core will work with the SDRC investigators of the various projects in experimental design and sample generation, to ensure optimal analysis. Thus, the specific aims of this Core are (1) Optimization of protein recovery from cells and tissue specimens, (2) High resolution protein separation, (3) Isoelectric focusing/SDS-PAGE electrophoresis (on three different 2 nd dimension gel sizes), (4) Qualitative and quantitative gel image analysis via software (currently PDQuest [BioRad]) to identify differential protein expression and/or alterations in posttranslational modifications, (5) MALDI-TOF mass spectrometric identification of polypeptides based on database searching with tryptic mass fingerprints, (6) NanoLC-electrospray ionization mass spectrometry for peptide sequence analysis and analysis of covalent modifications Training in the experimental design and application of proteomics, and (7) use of protein databases and bioinformatics. Overall, the goals of the proteomics core are (1) to provide high-resolution two dimensional electrophoretic and/or chromatographic protein separations coupled with MALDI-TOF and other mass spectrometry analyses, and (2) to identify differences in polypeptide expression and/or modifications that may be the basis for various dermatological conditions being studied by the Center investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
1P30AR050948-01
Application #
6762606
Study Section
Special Emphasis Panel (ZAR1-AAA-G (J1))
Project Start
2004-04-01
Project End
2009-03-30
Budget Start
2004-04-01
Budget End
2005-07-31
Support Year
1
Fiscal Year
2004
Total Cost
$108,750
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Type
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294

  Publications

Frugé, Andrew D; Van der Pol, William; Rogers, Laura Q et al. (2018) Fecal Akkermansia muciniphila Is Associated with Body Composition and Microbiota Diversity in Overweight and Obese Women with Breast Cancer Participating in a Presurgical Weight Loss Trial. J Acad Nutr Diet :
Frugé, Andrew D; Cases, Mallory G; Howell, Carrie R et al. (2018) Fingernail and toenail clippings as a non-invasive measure of chronic cortisol levels in adult cancer survivors. Cancer Causes Control 29:185-191
Stoll, Matthew L; Pierce, M Kathy; Watkins, Jordan A et al. (2018) Akkermansia muciniphila is permissive to arthritis in the K/BxN mouse model of arthritis. Genes Immun :
Frugé, Andrew D; Ptacek, Travis; Tsuruta, Yuko et al. (2018) Dietary Changes Impact the Gut Microbe Composition in Overweight and Obese Men with Prostate Cancer Undergoing Radical Prostatectomy. J Acad Nutr Diet 118:714-723.e1
Koo, Hyunmin; Hakim, Joseph A; Powell, Mickie L et al. (2017) Metagenomics approach to the study of the gut microbiome structure and function in zebrafish Danio rerio fed with gluten formulated diet. J Microbiol Methods 135:69-76
Garcia, S S; Blackledge, M S; Michalek, S et al. (2017) Targeting of Streptococcus mutans Biofilms by a Novel Small Molecule Prevents Dental Caries and Preserves the Oral Microbiome. J Dent Res 96:807-814
Larson, Thomas R; Yother, Janet (2017) Streptococcus pneumoniae capsular polysaccharide is linked to peptidoglycan via a direct glycosidic bond to ?-D-N-acetylglucosamine. Proc Natl Acad Sci U S A 114:5695-5700
Brawner, K M; Kumar, R; Serrano, C A et al. (2017) Helicobacter pylori infection is associated with an altered gastric microbiota in children. Mucosal Immunol 10:1169-1177
Kim, T; Holleman, C L; Ptacek, T et al. (2017) Duodenal endoluminal barrier sleeve alters gut microbiota of ZDF rats. Int J Obes (Lond) 41:381-389
Sharma, Nirmal S; Wille, Keith M; Athira, S et al. (2017) Distal airway microbiome is associated with immunoregulatory myeloid cell responses in lung transplant recipients. J Heart Lung Transplant :

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