As part of the DAB Skin Diseases Research Center (SDRC), the mission of the DAB SDRC Skin Cell Culture Core (SCCC) is to provide valuable resources, services, and training in research techniques for promoting interdisciplinary research in cutaneous biology. Over the last 4 years, our core services have become a vital resource to a diverse community of 35 UAB SDRC members and 12 new investigators representing 22 departments who depend upon our expertise to facilitate and support studies relating to cutaneous biology. The SCCC faculty and research staff have established expertise in the generation and maintenance of purified epidermal and dermal primary cell subsets, derived primarily from murine and human skin tissues or biopsy samples, in monolayer or organotypic cultures. We propose to continue to support and promote cutaneous research at UAB with the following specific aims: 1) To provide a central source of epidermal and dermal cell types from murine and human tissues to Center members in a cost efficient manner. 2) To maintain quality control measures that ensure the distribution of standardized contamination-free cultures. 3) To provide expertise in generating specialized cell culture systems and transfecting skin cells from either human or mouse sources. 4) To optimize methods for continuous long-term culture and immortalization protocols of murine keratinocytes derived from wild type and mutant mouse strains to accommodate increased demand by SDRC members. By providing various cell types at a cost that encourages new exploration and innovation, the SDRC SCCC has been essential for enhancing the quality and expanding the breadth of cutaneous research performed by multiple UAB investigators during last 4 years. We propose to continue and improve upon this vital service.

Public Health Relevance

The ability to provide skin cell cutlures and assistance in dermatologic research to investigators at UAB will faciliate new exploration that will expand the breadth of cutaneous research and promote innovative approaches that can lead to break throughs in treating dermatologic disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR050948-10
Application #
8538748
Study Section
Special Emphasis Panel (ZAR1-KM-D)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
10
Fiscal Year
2013
Total Cost
$123,770
Indirect Cost
$40,323
Name
University of Alabama Birmingham
Department
Type
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Pal, Harish Chandra; Athar, Mohammad; Elmets, Craig A et al. (2015) Fisetin inhibits UVB-induced cutaneous inflammation and activation of PI3K/AKT/NF?B signaling pathways in SKH-1 hairless mice. Photochem Photobiol 91:225-34
Nasti, Tahseen H; Timares, Laura (2015) MC1R, eumelanin and pheomelanin: their role in determining the susceptibility to skin cancer. Photochem Photobiol 91:188-200
Pal, H C; Chamcheu, J C; Adhami, V M et al. (2015) Topical application of delphinidin reduces psoriasiform lesions in the flaky skin mouse model by inducing epidermal differentiation and inhibiting inflammation. Br J Dermatol 172:354-64
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Wells, J Michael; O'Reilly, Philip J; Szul, Tomasz et al. (2014) An aberrant leukotriene A4 hydrolase-proline-glycine-proline pathway in the pathogenesis of chronic obstructive pulmonary disease. Am J Respir Crit Care Med 190:51-61
Elmets, Craig A; Cala, Cather M; Xu, Hui (2014) Photoimmunology. Dermatol Clin 32:277-90, vii
Chaudhary, Sandeep C; Singh, Tripti; Talwelkar, Sarang S et al. (2014) Erb-041, an estrogen receptor-* agonist, inhibits skin photocarcinogenesis in SKH-1 hairless mice by downregulating the WNT signaling pathway. Cancer Prev Res (Phila) 7:186-98
Arumugam, Aadithya; Weng, Zhiping; Chaudhary, Sandeep C et al. (2014) Keratin-6 driven ODC expression to hair follicle keratinocytes enhances stemness and tumorigenesis by negatively regulating Notch. Biochem Biophys Res Commun 451:394-401
Brawner, Kyle M; Morrow, Casey D; Smith, Phillip D (2014) Gastric microbiome and gastric cancer. Cancer J 20:211-6
Elmets, Craig A; Ledet, Johnathan J; Athar, Mohammad (2014) Cyclooxygenases: mediators of UV-induced skin cancer and potential targets for prevention. J Invest Dermatol 134:2497-502

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