As part of the DAB Skin Diseases Research Center (SDRC), the mission of the DAB SDRC Skin Cell Culture Core (SCCC) is to provide valuable resources, services, and training in research techniques for promoting interdisciplinary research in cutaneous biology. Over the last 4 years, our core services have become a vital resource to a diverse community of 35 UAB SDRC members and 12 new investigators representing 22 departments who depend upon our expertise to facilitate and support studies relating to cutaneous biology. The SCCC faculty and research staff have established expertise in the generation and maintenance of purified epidermal and dermal primary cell subsets, derived primarily from murine and human skin tissues or biopsy samples, in monolayer or organotypic cultures. We propose to continue to support and promote cutaneous research at UAB with the following specific aims: 1) To provide a central source of epidermal and dermal cell types from murine and human tissues to Center members in a cost efficient manner. 2) To maintain quality control measures that ensure the distribution of standardized contamination-free cultures. 3) To provide expertise in generating specialized cell culture systems and transfecting skin cells from either human or mouse sources. 4) To optimize methods for continuous long-term culture and immortalization protocols of murine keratinocytes derived from wild type and mutant mouse strains to accommodate increased demand by SDRC members. By providing various cell types at a cost that encourages new exploration and innovation, the SDRC SCCC has been essential for enhancing the quality and expanding the breadth of cutaneous research performed by multiple UAB investigators during last 4 years. We propose to continue and improve upon this vital service.
The ability to provide skin cell cutlures and assistance in dermatologic research to investigators at UAB will faciliate new exploration that will expand the breadth of cutaneous research and promote innovative approaches that can lead to break throughs in treating dermatologic disorders.
|Tanner, Scott M; Daft, Joseph G; Hill, Stephanie A et al. (2016) Altered T-Cell Balance in Lymphoid Organs of a Mouse Model of Colorectal Cancer. J Histochem Cytochem 64:753-767|
|Stoll, M L; Kumar, R; Lefkowitz, E J et al. (2016) Fecal metabolomics in pediatric spondyloarthritis implicate decreased metabolic diversity and altered tryptophan metabolism as pathogenic factors. Genes Immun 17:400-405|
|Subramaniam, Akila; Kumar, Ranjit; Cliver, Suzanne P et al. (2016) Vaginal Microbiota in Pregnancy: Evaluation Based on Vaginal Flora, Birth Outcome, and Race. Am J Perinatol 33:401-8|
|Li, Xingsheng; Ellis, Melissa L; Dowell, Alexander E et al. (2016) Response of germ-free mice to colonization with O. formigenes and altered Schaedler flora. Appl Environ Microbiol :|
|Edwards, Rodney K; Kumar, Ranjit; Zhi, Degui et al. (2016) Gravidas with class III obesity: evaluating the abdominal skin microbiota above and below the panniculus (.). J Matern Fetal Neonatal Med 29:3312-6|
|Kumar, Ranjit; Maynard, Craig L; Eipers, Peter et al. (2016) Colonization potential to reconstitute a microbe community in patients detected early after fecal microbe transplant for recurrent C. difficile. BMC Microbiol 16:5|
|Wu, Lizhi; Chaudhary, Sandeep C; Atigadda, Venkatram R et al. (2016) Retinoid X Receptor Agonists Upregulate Genes Responsible for the Biosynthesis of All-Trans-Retinoic Acid in Human Epidermis. PLoS One 11:e0153556|
|Demark-Wahnefried, Wendy; Nix, Jeffery W; Hunter, Gary R et al. (2016) Feasibility outcomes of a presurgical randomized controlled trial exploring the impact of caloric restriction and increased physical activity versus a wait-list control on tumor characteristics and circulating biomarkers in men electing prostatectomy for BMC Cancer 16:61|
|Lal, Charitharth Vivek; Travers, Colm; Aghai, Zubair H et al. (2016) The Airway Microbiome at Birth. Sci Rep 6:31023|
|Berman, Brian; Ellis, Charles; Elmets, Craig (2016) Polypodium Leucotomos--An Overview of Basic Investigative Findings. J Drugs Dermatol 15:224-8|
Showing the most recent 10 out of 210 publications