The Structure and composition of musculoskeletal tissues are tailored to meet their demanding functions. With injury, the structure and composition of these tissues deteriorate, resulting in a decline or loss of mechanical function. Musculoskeletal tissues each have a wide array of compositional and structural variety with respect to collagen and proteoglycan types, as well as other extracellular matrix constituents and factors. Careful description and quantification of tissue structural organization and composition, as well as localization and identification of growth factors and cytokines, are necessary requirements for elucidation of the biologic mechanisms underlying musculoskeletal integrity, injury, and repair. The overall objective of this Histology Core (HC) is to develop and utilize a wide range of histological and histomorphometric approaches to evaluate musculoskeletal tissue injury and repair, and to provide training and funding for new projects and collaborations utilizing these assays.
The Specific Aims are:
Aim 1 : To provide guidance and training on the capabilities, advantages, and disadvantages of the various methodologies to assess musculoskeletal tissue structure and composition through formal educational enrichment programs and one-on-one interactions.
Aim 2 : To provide expertise and service for histological and histomorphometric assays of musculoskeletal tissues.
Aim 3 : To develop new histologically-based techniques that will be applicable to musculoskeletal research.
Aim 4 : To provide funding for development of new projects and collaborations and to develop preliminary and/or feasibility data for investigators.

Public Health Relevance

Successful completion of these aims will enhance the environment and the capabilities of researchers in the Penn Center for Musculoskeletal Disorders, leading to new approaches to address musculoskeletal disorders and new collaborations between Center faculty who may have not previously included structural and compositional approaches through histological examination in their musculoskeletal research programs.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30AR050950-09
Application #
8681154
Study Section
Special Emphasis Panel (ZAR1)
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Heo, Su-Jin; Driscoll, Tristan P; Thorpe, Stephen D et al. (2016) Differentiation alters stem cell nuclear architecture, mechanics, and mechano-sensitivity. Elife 5:
Huegel, Julianne; Kim, Dong Hwa; Cirone, James M et al. (2016) Autologous tendon-derived cell-seeded nanofibrous scaffolds improve rotator cuff repair in an age-dependent fashion. J Orthop Res :
Connizzo, Brianne K; Adams, Sheila M; Adams, Thomas H et al. (2016) Multiscale regression modeling in mouse supraspinatus tendons reveals that dynamic processes act as mediators in structure-function relationships. J Biomech 49:1649-57
Connizzo, Brianne K; Adams, Sheila M; Adams, Thomas H et al. (2016) Collagen V expression is crucial in regional development of the supraspinatus tendon. J Orthop Res 34:2154-2161
McLeod, Claire M; Mauck, Robert L (2016) High fidelity visualization of cell-to-cell variation and temporal dynamics in nascent extracellular matrix formation. Sci Rep 6:38852
Pardes, A M; Freedman, B R; Fryhofer, G W et al. (2016) Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model. Ann Biomed Eng 44:2901-10
Heo, Su-Jin; Han, Woojin M; Szczesny, Spencer E et al. (2016) Mechanically Induced Chromatin Condensation Requires Cellular Contractility in Mesenchymal Stem Cells. Biophys J 111:864-74
Tucker, Jennica J; Riggin, Corinne N; Connizzo, Brianne K et al. (2016) Effect of overuse-induced tendinopathy on tendon healing in a rat supraspinatus repair model. J Orthop Res 34:161-6
Saxena, Vishal; Kim, Minwook; Keah, Niobra M et al. (2016) Anatomic Mesenchymal Stem Cell-Based Engineered Cartilage Constructs for Biologic Total Joint Replacement. Tissue Eng Part A 22:386-95
Han, Woojin M; Heo, Su-Jin; Driscoll, Tristan P et al. (2016) Microstructural heterogeneity directs micromechanics and mechanobiology in native and engineered fibrocartilage. Nat Mater 15:477-84

Showing the most recent 10 out of 217 publications