Genetically engineered mice have become an essential tool in biomedical research. They are crucial tools for studying development of the skin and its appendages. In addition, these mouse models have become indispensible for creating models of human skin disorders, including inherited and aquired skin disorders and skin cancer. Generating transgenic or knockout mice requires specialized skills and expensive equipment. Consequently, very few laboratories have the ability to generate these valuable research models. The Transgenic and Gene Targeting (TGT) Core at the University of Colorado Denver offers a comprehensive set of services which enables investigators to generate genetically engineered mice. These services include the generation of transgenic mice via pronuclear injections, and gene targeting experiments that include the entire process from generating recombinant ES cells to the production of chimeric mice. All that is required from the investigators to initiate these experiments is a transgenic vector or gene targeting vector. Furthermore, our core offers extensive consultation services to help investigators with the development of a research plan, including vector design, animal design, and the analysis of genetically engineered mice. 23 investigators of the Skin Disease Research Core Center (UCD-SDRC) are planning to use our core services to generate mouse models to study skin biology or skin diseases. Core Center support for our core will provide these investigators with subsidized access to the full set of our services, and thus facilitate their research.

Public Health Relevance

The services of the proposed core component (Transgenic and Gene Targeting Core) are essential for the ability of Core Center investigators to generate animal models for skin diseases. Funds provided to the Transgenic and Gene Targeting Core will provide access of Core Center members to subsidized core services such as pronuclear injections to generate transgenic mice and ES cell injections to generate knockout and knockin mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR057212-04
Application #
8376561
Study Section
Special Emphasis Panel (ZAR1-KM-D)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
4
Fiscal Year
2012
Total Cost
$157,513
Indirect Cost
$54,563
Name
University of Colorado Denver
Department
Type
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045
Gaskill, Christa F; Carrier, Erica J; Kropski, Jonathan A et al. (2017) Disruption of lineage specification in adult pulmonary mesenchymal progenitor cells promotes microvascular dysfunction. J Clin Invest 127:2262-2276
Miller, Shannon M; Miles, Brodie; Guo, Kejun et al. (2017) Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1. J Virol 91:
Mukherjee, Nabanita; Lu, Yan; Almeida, Adam et al. (2017) Use of a MCL-1 inhibitor alone to de-bulk melanoma and in combination to kill melanoma initiating cells. Oncotarget 8:46801-46817
Yang, N; Leung, E L-H; Liu, C et al. (2017) INTU is essential for oncogenic Hh signaling through regulating primary cilia formation in basal cell carcinoma. Oncogene 36:4997-5005
Birlea, Stanca A; Costin, Gertrude-E; Roop, Dennis R et al. (2017) Trends in Regenerative Medicine: Repigmentation in Vitiligo Through Melanocyte Stem Cell Mobilization. Med Res Rev 37:907-935
Zhai, Z; Liu, W; Kaur, M et al. (2017) NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene 36:3820-3830
Reynolds, Susan D; Rios, Cydney; Wesolowska-Andersen, Agata et al. (2016) Airway Progenitor Clone Formation Is Enhanced by Y-27632-Dependent Changes in the Transcriptome. Am J Respir Cell Mol Biol 55:323-36
Goldstein, Nathaniel B; Koster, Maranke I; Hoaglin, Laura G et al. (2016) Isolating RNA from precursor and mature melanocytes from human vitiligo and normal skin using laser capture microdissection. Exp Dermatol 25:805-11
Morton, J J; Bird, G; Keysar, S B et al. (2016) XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer. Oncogene 35:290-300
Du, L; Chen, X; Cao, Y et al. (2016) Overexpression of PIK3CA in murine head and neck epithelium drives tumor invasion and metastasis through PDK1 and enhanced TGF? signaling. Oncogene 35:4641-52

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