Flow Cytometry and cell sorting are integral and essential components of modern biomedical research. These techniques allow for the rapid identification of individual cells within a mixed population based on such properties as cell surface antigen expression, cell cycle status, cell signaling, or fluorescence- tagged transgenes. Furthermore, once cell populations are identified they can be isolated into discreet populations via cell sorting. Core E, Flow Cytometry, is operated under a cooperative arrangement with the University of Colorado Cancer Center Flow Cytometry Shared Resource (CC-FCSR).This state-of- the-art facility provides two instruments capable of high speed cell sorting based on up to 14 color parameters. Additionally, the CC-FCSR provides four flow cytometer analysis machines capable of up to 10- color analysis, two ViCell cell counters, and a Luminex Magpix system for multiplexed bead array analysis of cytokines, chemokines, and cell signaling proteins. The CC-FCSR personnel provide a consultation service for analysis and cell sorting strategies, cell preparation, and assay trouble-shooting. The Flow Cytometry Core has been used extensively by investigators involved in skin disease. The core is capable of identifying and isolating various skin cell subpopulations, such as keratinocytes and keratinocyte stem cell populations, as well as isolating normal and skin tumor stem cells from tissues or cell cultures for further in vitro and in vivo analysis. Additionally, investigators are able to identify, isolate, and further assess phenotype-specific T cell or other immune cell populations associated with various skin pathologies. Flow Cytometry Core center support insures that investigators of the UCAMC Skin Diseases Research Core have full access to all services provided by the CC-FCSR at a subsidized rate.

Public Health Relevance

The services of this core component allow UCAMC-SDRC investigators access to flow cytometric analysis of cells in skin disease models, both in vitro and in vivo. Moreover, the Flow Cytometry Core provides the only practical means of isolating rare stem cell populations or immune cell subsets from skin cell preparations or cell cultures. Funding of the Flow Cytometry Core will provide full access to core services at a subsidized rate

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
2P30AR057212-06
Application #
8753462
Study Section
Special Emphasis Panel (ZAR1-KM (M1))
Project Start
Project End
Budget Start
2014-08-20
Budget End
2015-07-31
Support Year
6
Fiscal Year
2014
Total Cost
$78,152
Indirect Cost
$27,745
Name
University of Colorado Denver
Department
Type
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045
Ishitsuka, Yosuke; Huebner, Aaron J; Rice, Robert H et al. (2016) Lce1 Family Members Are Nrf2-Target Genes that Are Induced to Compensate for the Loss of Loricrin. J Invest Dermatol 136:1656-63
Liu, Ying; Snedecor, Elizabeth R; Zhang, Xu et al. (2016) Correction of Hair Shaft Defects through Allele-Specific Silencing of Mutant Krt75. J Invest Dermatol 136:45-51
Reynolds, Susan D; Rios, Cydney; Wesolowska-Andersen, Agata et al. (2016) Airway Progenitor Clone Formation Is Enhanced by Y-27632-Dependent Changes in the Transcriptome. Am J Respir Cell Mol Biol 55:323-36
Mukherjee, Nabanita; Lu, Yan; Almeida, Adam et al. (2016) Use of a MCL-1 inhibitor alone to de-bulk melanoma and in combination to kill melanoma initiating cells. Oncotarget :
Tilley, Cynthia; Deep, Gagan; Agarwal, Chapla et al. (2016) Silibinin and its 2,3-dehydro-derivative inhibit basal cell carcinoma growth via suppression of mitogenic signaling and transcription factors activation. Mol Carcinog 55:3-14
Zhang, Lei; Ferreyros, Michael; Feng, Weiguo et al. (2016) Defects in Stratum Corneum Desquamation Are the Predominant Effect of Impaired ABCA12 Function in a Novel Mouse Model of Harlequin Ichthyosis. PLoS One 11:e0161465
Kohler, Stephanie L; Pham, Michael N; Folkvord, Joy M et al. (2016) Germinal Center T Follicular Helper Cells Are Highly Permissive to HIV-1 and Alter Their Phenotype during Virus Replication. J Immunol 196:2711-22
Jin, Ying; Andersen, Genevieve; Yorgov, Daniel et al. (2016) Genome-wide association studies of autoimmune vitiligo identify 23 new risk loci and highlight key pathways and regulatory variants. Nat Genet 48:1418-1424
Du, L; Chen, X; Cao, Y et al. (2016) Overexpression of PIK3CA in murine head and neck epithelium drives tumor invasion and metastasis through PDK1 and enhanced TGFβ signaling. Oncogene 35:4641-52
Morton, J J; Bird, G; Keysar, S B et al. (2016) XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer. Oncogene 35:290-300

Showing the most recent 10 out of 48 publications