The role of the Tissue and Keratinocyte Procurement Core is to facilitate the acquisition of primary tissue and keratinocytes from normal human skin, patient specimens, and genetically-engineered mice. Researchers who discover a skin phenotype in genetically-engineered mice need to identify whether a similar phenotype is observed in human disease. Additionally, researchers who want to determine if a particular molecule or pathway is involved in skin physiology require primary keratinocytes from geneticallyengineered mice for analysis. Skin biologists often use human skin models, including skin organ culture and human skin xenografts, which requires continuous procurement of normal human skin. To facilitate these studies, core B will provide expertise, service, and training in obtaining these primary tissues and the relevant IRB approvals. Since primary keratinocyte cultures are derived from freshly isolated human skin, the core can distribute these tissues using cost-effective and uniform methods. Additionally, Core B will establish a tissue bank from patient skin biopsy specimens stored in the dermatopathology clinical laboratory. When a skin or hair follicle phenotype is identified, relevant paraffin embedded samples from the Core B tissue bank will be identified and provided to Core A (Skin Histology and Characterization) for sectioning and histopathological analysis. Core B will also coordinate with Core C (Stem Cell and Xenograft) to procure normal human skin for xenografting. The unique services provided by core B therefore ensure the efficient distribution of primary tissues and facilitate translational research in animal and human models of skin disease, especially for Center researchers with no experience in skin biology or access to patient specimens.
The Aims of the core are: 1) To provide expertise, service and training in obtaining primary keratinocyte cultures, 2) To provide expertise and service in obtaining formalin-fixed sections of normal human skin and human skin diseases by establishing a tissue bank of patient skin biopsy specimens, and 3) To provide freshly isolated normal human skin for physiologic and pathophysiologic studies.

Public Health Relevance

The goal of the Tissue and Keratinocyte Procurement Core is to facilitate skin disease research through the efficient and equitable distribution of normal human skin, patient skin disease specimens, and primary keratinocytes. The core helps to encourage translational research in animal and human models of skin disease, even for investigators with no experience in skin biology or access to patient specimens.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR057217-05
Application #
8499264
Study Section
Special Emphasis Panel (ZAR1-KM-D)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
5
Fiscal Year
2013
Total Cost
$113,583
Indirect Cost
$42,593
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Ellebrecht, Christoph T; Bhoj, Vijay G; Nace, Arben et al. (2016) Reengineering chimeric antigen receptor T cells for targeted therapy of autoimmune disease. Science 353:179-84
Lo, Agnes S; Mao, Xuming; Mukherjee, Eric M et al. (2016) Pathogenicity and Epitope Characteristics Do Not Differ in IgG Subclass-Switched Anti-Desmoglein 3 IgG1 and IgG4 Autoantibodies in Pemphigus Vulgaris. PLoS One 11:e0156800
Cho, Michael Jeffrey; Ellebrecht, Christoph T; Hammers, Christoph M et al. (2016) Determinants of VH1-46 Cross-Reactivity to Pemphigus Vulgaris Autoantigen Desmoglein 3 and Rotavirus Antigen VP6. J Immunol 197:1065-73
Billings, Paul C; Sanzari, Jenine K; Kennedy, Ann R et al. (2015) Comparative analysis of colorimetric staining in skin using open-source software. Exp Dermatol 24:157-9
Suzuki, Daisuke; Sahu, Raju; Leu, N Adrian et al. (2015) The carboxy-terminus of p63 links cell cycle control and the proliferative potential of epidermal progenitor cells. Development 142:282-90
Gay, Denise L; Yang, Chao-Chun; Plikus, Maksim V et al. (2015) CD133 expression correlates with membrane beta-catenin and E-cadherin loss from human hair follicle placodes during morphogenesis. J Invest Dermatol 135:45-55
Ortiz, Myrna L; Kumar, Vinit; Martner, Anna et al. (2015) Immature myeloid cells directly contribute to skin tumor development by recruiting IL-17-producing CD4+ T cells. J Exp Med 212:351-67
Agarwal, Priti; Rashighi, Mehdi; Essien, Kingsley I et al. (2015) Simvastatin prevents and reverses depigmentation in a mouse model of vitiligo. J Invest Dermatol 135:1080-8
Wong, Waihay J; Richardson, Theresa; Seykora, John T et al. (2015) Hypoxia-inducible factors regulate filaggrin expression and epidermal barrier function. J Invest Dermatol 135:454-61
Hammers, Christoph M; Chen, Jing; Lin, Chenyan et al. (2015) Persistence of anti-desmoglein 3 IgG(+) B-cell clones in pemphigus patients over years. J Invest Dermatol 135:742-9

Showing the most recent 10 out of 86 publications