The role of the Tissue and Keratinocyte Procurement Core is to facilitate the acquisition of primary tissue and keratinocytes from normal human skin, patient specimens, and genetically-engineered mice. Researchers who discover a skin phenotype in genetically-engineered mice need to identify whether a similar phenotype is observed in human disease. Additionally, researchers who want to determine if a particular molecule or pathway is involved in skin physiology require primary keratinocytes from geneticallyengineered mice for analysis. Skin biologists often use human skin models, including skin organ culture and human skin xenografts, which requires continuous procurement of normal human skin. To facilitate these studies, core B will provide expertise, service, and training in obtaining these primary tissues and the relevant IRB approvals. Since primary keratinocyte cultures are derived from freshly isolated human skin, the core can distribute these tissues using cost-effective and uniform methods. Additionally, Core B will establish a tissue bank from patient skin biopsy specimens stored in the dermatopathology clinical laboratory. When a skin or hair follicle phenotype is identified, relevant paraffin embedded samples from the Core B tissue bank will be identified and provided to Core A (Skin Histology and Characterization) for sectioning and histopathological analysis. Core B will also coordinate with Core C (Stem Cell and Xenograft) to procure normal human skin for xenografting. The unique services provided by core B therefore ensure the efficient distribution of primary tissues and facilitate translational research in animal and human models of skin disease, especially for Center researchers with no experience in skin biology or access to patient specimens.
The Aims of the core are: 1) To provide expertise, service and training in obtaining primary keratinocyte cultures, 2) To provide expertise and service in obtaining formalin-fixed sections of normal human skin and human skin diseases by establishing a tissue bank of patient skin biopsy specimens, and 3) To provide freshly isolated normal human skin for physiologic and pathophysiologic studies.

Public Health Relevance

The goal of the Tissue and Keratinocyte Procurement Core is to facilitate skin disease research through the efficient and equitable distribution of normal human skin, patient skin disease specimens, and primary keratinocytes. The core helps to encourage translational research in animal and human models of skin disease, even for investigators with no experience in skin biology or access to patient specimens.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Center Core Grants (P30)
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Special Emphasis Panel (ZAR1-KM-D)
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University of Pennsylvania
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Monteleon, Christine L; Agnihotri, Tanvir; Dahal, Ankit et al. (2018) Lysosomes Support the Degradation, Signaling, and Mitochondrial Metabolism Necessary for Human Epidermal Differentiation. J Invest Dermatol 138:1945-1954
Meisel, Jacquelyn S; Sfyroera, Georgia; Bartow-McKenney, Casey et al. (2018) Commensal microbiota modulate gene expression in the skin. Microbiome 6:20
Plikus, Maksim V; Guerrero-Juarez, Christian F; Ito, Mayumi et al. (2017) Regeneration of fat cells from myofibroblasts during wound healing. Science 355:748-752
Xu, Mingang; Horrell, Jeremy; Snitow, Melinda et al. (2017) WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation. Nat Commun 8:15397
Cho, Michael Jeffrey; Ellebrecht, Christoph T; Hammers, Christoph M et al. (2016) Determinants of VH1-46 Cross-Reactivity to Pemphigus Vulgaris Autoantigen Desmoglein 3 and Rotavirus Antigen VP6. J Immunol 197:1065-73
Hammers, Christoph M; Stanley, John R (2016) Mechanisms of Disease: Pemphigus and Bullous Pemphigoid. Annu Rev Pathol 11:175-97
Ellebrecht, Christoph T; Bhoj, Vijay G; Nace, Arben et al. (2016) Reengineering chimeric antigen receptor T cells for targeted therapy of autoimmune disease. Science 353:179-84
Geherin, Skye A; G├│mez, Daniela; Glabman, Raisa A et al. (2016) IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin. J Immunol 196:2514-2525
Lo, Agnes S; Mao, Xuming; Mukherjee, Eric M et al. (2016) Pathogenicity and Epitope Characteristics Do Not Differ in IgG Subclass-Switched Anti-Desmoglein 3 IgG1 and IgG4 Autoantibodies in Pemphigus Vulgaris. PLoS One 11:e0156800
Gay, Denise L; Yang, Chao-Chun; Plikus, Maksim V et al. (2015) CD133 expression correlates with membrane beta-catenin and E-cadherin loss from human hair follicle placodes during morphogenesis. J Invest Dermatol 135:45-55

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