Core C will support and encourage research into cutaneous epithelial stem cells by providing expertise and training in the characterization and isolation of epithelial stem cells in the hair follicle. The main goals of Core C are to enable investigators to analyze their transgenic or mutant mice for alterations in epithelial stem cells and to encourage translation of mouse work to human skin using a human skin/scid mouse xenograft model. Core C takes advantage of assays and expertise in stem cells found in the core director's laboratory, as well as existing resources and successful cores established at Penn. In particular, Core C will integrate with Penn's School of Medicine Flow Cytometry Core run out of the pathology department and the Xenografting core in the department of medicine that is partly funded through the Institute for Regenerative Medicine. These interactions will assure that investigators will have access to high quality, cost effective services, as well as the scientific expertise necessary to study epithelial stem cells in their research efforts. The stem cell core will offer analysis of hair follicle stem and progenitor cells using a wide variety of standard (label-retaining cell, immunohistochemistry) and cutting edge assays (marker analysis by fluorescent activated cell sorting [FACS],.multipotency testing). Core C will offer functional testing of hair follicle stem and progenitor cells in reconstitution assays. A major part of the core will be to provide training to laboratory personnel so that they can isolate cell populations from the skin and perform FACS analysis and cell isolation using the established Flow Cytometry Core. The human skin xenograft portion of Core C will encourage testing relevance of mouse findings in human skin. The xenograft model is an elegant means to perform preclinical functional studies on human skin that will streamline the development of clinically relevant technologies. Core C will integrate with Core A by using Core A's immunohistochemistry services to assess stem cell markers in tissues. Core C will integrate with Core B by receiving skin for xenografting.

Public Health Relevance

Core C will support the majority of SDRC members including several highly talented investigators outside the skin field who are interested in pursing questions related to cutaneous epithelial stem cells. Core C will offer analysis and isolation of stem cell populations, as well as the ability to study human skin grafted onto immunodeficient mice using sophisticated techniques available through medical school supported cores.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR057217-05
Application #
8499265
Study Section
Special Emphasis Panel (ZAR1-KM-D)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
5
Fiscal Year
2013
Total Cost
$143,122
Indirect Cost
$53,671
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Ellebrecht, Christoph T; Bhoj, Vijay G; Nace, Arben et al. (2016) Reengineering chimeric antigen receptor T cells for targeted therapy of autoimmune disease. Science 353:179-84
Lo, Agnes S; Mao, Xuming; Mukherjee, Eric M et al. (2016) Pathogenicity and Epitope Characteristics Do Not Differ in IgG Subclass-Switched Anti-Desmoglein 3 IgG1 and IgG4 Autoantibodies in Pemphigus Vulgaris. PLoS One 11:e0156800
Cho, Michael Jeffrey; Ellebrecht, Christoph T; Hammers, Christoph M et al. (2016) Determinants of VH1-46 Cross-Reactivity to Pemphigus Vulgaris Autoantigen Desmoglein 3 and Rotavirus Antigen VP6. J Immunol 197:1065-73
Billings, Paul C; Sanzari, Jenine K; Kennedy, Ann R et al. (2015) Comparative analysis of colorimetric staining in skin using open-source software. Exp Dermatol 24:157-9
Suzuki, Daisuke; Sahu, Raju; Leu, N Adrian et al. (2015) The carboxy-terminus of p63 links cell cycle control and the proliferative potential of epidermal progenitor cells. Development 142:282-90
Gay, Denise L; Yang, Chao-Chun; Plikus, Maksim V et al. (2015) CD133 expression correlates with membrane beta-catenin and E-cadherin loss from human hair follicle placodes during morphogenesis. J Invest Dermatol 135:45-55
Ortiz, Myrna L; Kumar, Vinit; Martner, Anna et al. (2015) Immature myeloid cells directly contribute to skin tumor development by recruiting IL-17-producing CD4+ T cells. J Exp Med 212:351-67
Agarwal, Priti; Rashighi, Mehdi; Essien, Kingsley I et al. (2015) Simvastatin prevents and reverses depigmentation in a mouse model of vitiligo. J Invest Dermatol 135:1080-8
Wong, Waihay J; Richardson, Theresa; Seykora, John T et al. (2015) Hypoxia-inducible factors regulate filaggrin expression and epidermal barrier function. J Invest Dermatol 135:454-61
Hammers, Christoph M; Chen, Jing; Lin, Chenyan et al. (2015) Persistence of anti-desmoglein 3 IgG(+) B-cell clones in pemphigus patients over years. J Invest Dermatol 135:742-9

Showing the most recent 10 out of 86 publications