Minnesota CCMBM Core B: Muscle Histology and Clinical Repository Core The University of Minnesota Muscular Dystrophy Clinic cares for one of the largest groups of muscular dystrophy patients in the country. Coupled with the comprehensive genetic and histological diagnostic methods developed and maintained by the MDClinic, this population provides a valuable resource for the MDCenter and this NIAMS Core Center for Musculoskeletal Biology and Medicine. As part of its comprehensive clinical diagnostic facility, the MDClinic has established a repository of DNA, fibroblasts, myoblasts, lymphoblastoid cell lines and residual muscle biopsy tissue from subjects with muscle diseases;if specifically supported for research purposes, this Core will provide great value to many currently funded MDCenter research programs, and will be a valuable asset to the greater MD Research Community.
Specific Aims of this Core are: 1) Expand the Muscular Dystrophy Database and DNA/Cell Culture/Tissue Repository. Formally establishing this as a research core will facilitate acquisition and distribution of many more samples. 2) To make state-of-the-art histological techniques accessible to fully and complementarity characterize muscle from patients and from animals that model human muscle disease. These methods will allow detailed characterization of diseased muscle as well as comparative studies after treatment in patients and model systems. 3) To provide tissue or cultured cells for detailed analysis of muscle structure and function. MDCenter research on clinically defined specimens will be of value to many basic and translational programs. Specifically, fibroblasts can be studied directly, differentiated into myoblasts for a full range of genetic, biochemical and force generation (Core C) or used to generate IPS cells (Pilot 1) for developmental studies along multiple cell lineages in specified genetic disorder

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR057220-05
Application #
8508073
Study Section
Special Emphasis Panel (ZAR1-CHW-G)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
5
Fiscal Year
2013
Total Cost
$186,099
Indirect Cost
$62,855
Name
University of Minnesota Twin Cities
Department
Type
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
O'Rourke, Allison R; Lindsay, Angus; Tarpey, Michael D et al. (2018) Impaired muscle relaxation and mitochondrial fission associated with genetic ablation of cytoplasmic actin isoforms. FEBS J 285:481-500
Saunders, Cosmo A; Harris, Nathan J; Willey, Patrick T et al. (2017) TorsinA controls TAN line assembly and the retrograde flow of dorsal perinuclear actin cables during rearward nuclear movement. J Cell Biol 216:657-674
Houang, Evelyne M; Haman, Karen J; Kim, Mihee et al. (2017) Chemical End Group Modified Diblock Copolymers Elucidate Anchor and Chain Mechanism of Membrane Stabilization. Mol Pharm 14:2333-2339
Hebert, Sadie L; Fitzpatrick, Krysta R; McConnell, Samantha A et al. (2017) Effects of retinoic acid signaling on extraocular muscle myogenic precursor cells in vitro. Exp Cell Res 361:101-111
Saunders, Cosmo A; Luxton, G W Gant (2016) LINCing defective nuclear-cytoskeletal coupling and DYT1 dystonia. Cell Mol Bioeng 9:207-216
McCaffrey, Jesse E; James, Zachary M; Svensson, Bengt et al. (2016) A bifunctional spin label reports the structural topology of phospholamban in magnetically-aligned bicelles. J Magn Reson 262:50-56
Chan, Sunny Sun-Kin; Hagen, Hannah R; Swanson, Scott A et al. (2016) Development of Bipotent Cardiac/Skeletal Myogenic Progenitors from MESP1+ Mesoderm. Stem Cell Reports 6:26-34
Houang, Evelyne M; Haman, Karen J; Filareto, Antonio et al. (2015) Membrane-stabilizing copolymers confer marked protection to dystrophic skeletal muscle in vivo. Mol Ther Methods Clin Dev 2:15042
McCaffrey, Jesse E; James, Zachary M; Thomas, David D (2015) Optimization of bicelle lipid composition and temperature for EPR spectroscopy of aligned membranes. J Magn Reson 250:71-75
Muretta, Joseph M; Rohde, John A; Johnsrud, Daniel O et al. (2015) Direct real-time detection of the structural and biochemical events in the myosin power stroke. Proc Natl Acad Sci U S A 112:14272-7

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