Tissue structure and strength are the most relevant properties when assessing animal models related to musculoskeletal injury, disease and repair. Whether the treatment effects are created by genetic manipulation, metabolic challenge, simulated injury, surgical repair or other intervention, the net effects must be judged based on whether they have resulted in more or less tissue, whether that tissue has normal morphology, and whether the tissue or skeletal structure has increased or decreased mechanical properties. We have the necessary equipment and the demonstrated user expertise to assess the structural and mechanical properties of a range of musculoskeletal tissue and structures derived from animal models. There have been productive collaborations between investigators in the Research Base and the personnel of this Core, but these have been limited in number by the lack of a mechanism to support the acquisition and analysis of data by expert users and the training of non-expert users. The objective of this Musculoskeletal Structure and Strength Core (Core B) is to provide a mechanism to increase access to existing resources for x-ray based densitometry and imaging, and mechanical testing, and to thereby enable new interactions and enhance existing interactions between musculoskeletal researchers at Washington University. We will maintain protocols and equipment to promote quality control, provide technical support and training, and perform the following services on musculoskeletal structures and tissues from animal models (mouse to canine) generated by investigators in the Core Center Research Base.
Aim 1 : X-ray based imaging; available modalities include radiography, dual-energy x-ray absorptiometry (DXA), peripheral quantitative computed tomography (pQCT) and micro-computed tomography (microCT). Each of these is available for ex vivo or in vivo imaging.
Aim 2 : Mechanical testing;available modalities include whole-bone bending and compression, trabecular indentation, tensile testing of demineralized bone, tendon and tendon-bone insertion site tensile testing;muscle force measurement. By offering these Core services we will increase research productivity of established musculoskeletal investigators on our campus and facilitate non-musculoskeletal investigators wishing to bring new perspectives to studies in musculoskeletal biology and medicine.

Public Health Relevance

Musculoskeletal disorders such as osteoarthritis, osteoporosis and muscular dystrophy are a main cause of pain and suffering leading to diminished quality and lost time from work. Our research uses animal models to understand the biological factors underlying musculoskeletal disorders. We use imaging and mechanical testing techniques to assess the structure and strength of bone, tendon and muscle.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZAR1-CHW-G)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Washington University
Saint Louis
United States
Zip Code
Fontana, Francesca; Hickman-Brecks, Cynthia L; Salazar, Valerie S et al. (2017) N-cadherin Regulation of Bone Growth and Homeostasis Is Osteolineage Stage-Specific. J Bone Miner Res 32:1332-1342
Liu, Jennifer W; Lin, Kevin H; Weber, Christian et al. (2017) An In Vitro Organ Culture Model of the Murine Intervertebral Disc. J Vis Exp :
Liu, Xiaochen; McKenzie, Jennifer A; Maschhoff, Clayton W et al. (2017) Exogenous hedgehog antagonist delays but does not prevent fracture healing in young mice. Bone 103:241-251
Hibino, Itaru; Tang, Simon (2017) Differential Carbonyl Stress Expression in the Intervertebral Disc between Singular- and Persistent-Mechanical Injuries. Nagoya Gakuin Daigaku Ronshu Igaku Kenko Kagaku Supotsu Kagakuh 5:11-19
He, Guangxu; Shi, Yu; Lim, Joohyun et al. (2017) Differential involvement of Wnt signaling in Bmp regulation of cancellous versus periosteal bone growth. Bone Res 5:17016
Anderson, Britta A; McAlinden, Audrey (2017) miR-483 targets SMAD4 to suppress chondrogenic differentiation of human mesenchymal stem cells. J Orthop Res 35:2369-2377
Shi, Yu; He, Guangxu; Lee, Wen-Chih et al. (2017) Gli1 identifies osteogenic progenitors for bone formation and fracture repair. Nat Commun 8:2043
Mutneja, Anubha; Cossey, L Nicholas; Liapis, Helen et al. (2017) A rare case of renal thrombotic microangiopathy associated with Castleman's disease. BMC Nephrol 18:57
Kim, Yeawon; Park, Sun-Ji; Chen, Ying Maggie (2017) Mesencephalic astrocyte-derived neurotrophic factor (MANF), a new player in endoplasmic reticulum diseases: structure, biology, and therapeutic roles. Transl Res 188:1-9
Kim, Yeawon; Park, Sun-Ji; Manson, Scott R et al. (2017) Elevated urinary CRELD2 is associated with endoplasmic reticulum stress-mediated kidney disease. JCI Insight 2:

Showing the most recent 10 out of 279 publications