Mouse genetic models have been extensively utilized by CCBMB investigators and are central to research in musculoskeletal biology and medicine. The Animal Models Core aims to foster a state-of-the-art research environment for CCMBM researchers by supporting the optimization and implementation of new technologies, the generation of mouse genetic tools that will have wide use among CCBMB researchers, the production, preservation, and sharing of genetically altered mice in a timely and reliable manner, and the exploration of the zebrafish as a model for musculoskeletal research. To enhance the effective use of mouse and zebrafish model organisms for research in musculoskeletal biology, the Animal Models Core will facilitate the implementation of TALEN and CRISPR/Cas genomic editing technology. This technology will benefit CCBMB investigators by facilitating investigator-initiated development of mouse and zebrafish animal models, and by developing "universal" TALENs for targeting Cre recombinase, LacZ, and the ROSA26 locus, to allow efficient re-engineering of well-characterized mouse alleles. Establishing this genomic editing technology will benefit CCBMB members by enabling fast and cost-effective production of new mouse and zebrafish models and by allowing the genetic modification of well-characterized existing mouse lines that have well-defined temporal and spatial expression patterns. Additional aims of the Animal Models Core focus on consultation and education and the maintenance of a comprehensive database to foster sharing of animal models and genetic tools.

Public Health Relevance

Animal models provide essential tools for research in musculoskeletal biology. The zebrafish and mouse provide complementary organisms that together provide physiologically relevant models for musculoskeletal development and disease and allow the identification of novel gene functions through genetic screens. The Animal Models Core aims to facilitate and enhance the use of these model organisms for MRC investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
2P30AR057235-06
Application #
8708695
Study Section
Special Emphasis Panel ()
Project Start
Project End
Budget Start
2014-04-05
Budget End
2015-03-31
Support Year
6
Fiscal Year
2014
Total Cost
$52,932
Indirect Cost
$18,194
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Diez-Perez, A; Bouxsein, M L; Eriksen, E F et al. (2016) Technical note: Recommendations for a standard procedure to assess cortical bone at the tissue-level in vivo using impact microindentation. Bone Rep 5:181-185
Gelberman, Richard H; Shen, Hua; Kormpakis, Ioannis et al. (2016) Effect of adipose-derived stromal cells and BMP12 on intrasynovial tendon repair: A biomechanical, biochemical, and proteomics study. J Orthop Res 34:630-40
Blanton, Laura V; Charbonneau, Mark R; Salih, Tarek et al. (2016) Gut bacteria that prevent growth impairments transmitted by microbiota from malnourished children. Science 351:
Singh, Sudhir; Manson, Scott R; Lee, Heedoo et al. (2016) Tubular Overexpression of Angiopoietin-1 Attenuates Renal Fibrosis. PLoS One 11:e0158908
Agapova, Olga A; Fang, Yifu; Sugatani, Toshifumi et al. (2016) Ligand trap for the activin type IIA receptor protects against vascular disease and renal fibrosis in mice with chronic kidney disease. Kidney Int 89:1231-43
Kim, Yeawon; Lee, Heedoo; Manson, Scott R et al. (2016) Mesencephalic Astrocyte-Derived Neurotrophic Factor as a Urine Biomarker for Endoplasmic Reticulum Stress-Related Kidney Diseases. J Am Soc Nephrol 27:2974-2982
Black, James C; Ricci, William M; Gardner, Michael J et al. (2016) Novel Augmentation Technique for Patellar Tendon Repair Improves Strength and Decreases Gap Formation: A Cadaveric Study. Clin Orthop Relat Res 474:2611-2618
Kormpakis, Ioannis; Linderman, Stephen W; Thomopoulos, Stavros et al. (2016) Enhanced Zone II Flexor Tendon Repair through a New Half Hitch Loop Suture Configuration. PLoS One 11:e0153822
Yan, Huimin; Duan, Xin; Pan, Hua et al. (2016) Suppression of NF-κB activity via nanoparticle-based siRNA delivery alters early cartilage responses to injury. Proc Natl Acad Sci U S A 113:E6199-E6208
Shashkova, Elena V; Trivedi, Jahnavi; Cline-Smith, Anna B et al. (2016) Osteoclast-Primed Foxp3+ CD8 T Cells Induce T-bet, Eomesodermin, and IFN-γ To Regulate Bone Resorption. J Immunol 197:726-35

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