A Cell Culture Core is proposed to serve as a central facility for both quality controlled material resources and expert technical support in cell culture to investigators of the UWSDRC. This core will facilitate efficient execution of projects involving the use and study of skin cells from normal individuals and patients with skin diseases and murine genetic models of human skin disorders. The specific functions of this core are: 1) Provide quality-assured cultures of the three major cell types of the skin- keratinocytes, fibroblasts and melanocytes from normal and diseased human skin and mouse models of human skin disorders. These are important and critical resources for cell and molecular studies on cell death and differentiation. The Core will culture, expand and cryopreserve the 3 cell types as matched sets, when available, that will serve as isogenic controls. 2) Generate induced pluripotent stem cells (iPSCs) from skin cells. Skin derived iPSCs are useful for studies involving regulation of skin cell differentiation and modeling genetic skin diseases in vitro. The differentiated cells can be studied either as monolayers or after their incorporation into 3-D organotypic cultures. The Core will provide the required material resources and technical expertise to investigators and hands-on training, as needed. 3) Generate quality controlled lentiviruses. High titer lentiviruses have become indispensable reagents for effective gene transfer into primary cells. The UWSDRC Cell Culture Core will provide both material resources such as high quality packaging cells and technical expertise for production of viruses. 4) Provide technical expertise and hands-on training to investigators in manipulation of skin cells in culture. Many studies of cell death and differentiation involve transfection/electroporation with plasmid DNA or small inhibitory RNA or transduction with lentiviruses. 5) Provide cultured cells as source for macromolecules. Biochemical and molecular biological studies of cell death and differentiation of skin cells often require macromolecules (DNA, RNA or protein) isolated from individual cell types. The Core will provide frozen or fresh cell pellets for extraction of macromolecules of interest.

Public Health Relevance

Skin diseases constitute a major public health burden in the US and worldwide. Understanding the cellular and molecular basis of these diseases is facilitated by availability of quality controlled pure populations of skin cells derived from both healthy and affected individuals. The Cell Culture Core proposed here will serve a large interdisciplinary group of scientists in their studies of human skin biology in health and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
1P30AR066524-01
Application #
8753349
Study Section
Special Emphasis Panel (ZAR1-KM (M1))
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-06-30
Support Year
1
Fiscal Year
2014
Total Cost
$64,001
Indirect Cost
$22,170
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Rady, Islam; Bloch, Melissa B; Chamcheu, Roxane-Cherille N et al. (2018) Anticancer Properties of Graviola (Annona muricata): A Comprehensive Mechanistic Review. Oxid Med Cell Longev 2018:1826170
Karrys, Amitis; Rady, Islam; Chamcheu, Roxane-Cherille N et al. (2018) Bioactive Dietary VDR Ligands Regulate Genes Encoding Biomarkers of Skin Repair That Are Associated with Risk for Psoriasis. Nutrients 10:
Rodríguez, Carlos I; Castro-Pérez, Edgardo; Longley, B Jack et al. (2018) Elevated cyclic AMP levels promote BRAFCA/Pten-/- mouse melanoma growth but pCREB is negatively correlated with human melanoma progression. Cancer Lett 414:268-277
Khan, Mohammad Imran; Rath, Suvasmita; Adhami, Vaqar Mustafa et al. (2018) Hypoxia driven glycation: Mechanisms and therapeutic opportunities. Semin Cancer Biol 49:75-82
Meyers, Jordan M; Grace, Miranda; Uberoi, Aayushi et al. (2018) Inhibition of TGF-? and NOTCH Signaling by Cutaneous Papillomaviruses. Front Microbiol 9:389
Chhabra, Gagan; Garvey, Debra R; Singh, Chandra K et al. (2018) Effects and Mechanism of Nicotinamide Against UVA- and/or UVB-mediated DNA Damages in Normal Melanocytes. Photochem Photobiol :
Chamcheu, Jean Christopher; Siddiqui, Imtiaz A; Adhami, Vaqar M et al. (2018) Chitosan-based nanoformulated (-)-epigallocatechin-3-gallate (EGCG) modulates human keratinocyte-induced responses and alleviates imiquimod-induced murine psoriasiform dermatitis. Int J Nanomedicine 13:4189-4206
Rank, Leslie A; Walsh, Naomi M; Lim, Fang Yun et al. (2018) Peptide-Like Nylon-3 Polymers with Activity against Phylogenetically Diverse, Intrinsically Drug-Resistant Pathogenic Fungi. mSphere 3:
Zhao, Lei; Okhovat, Jean-Phillip; Hong, Eric K et al. (2018) Preclinical Studies Support Combined Inhibition of BET Family Proteins and Histone Deacetylases as Epigenetic Therapy for Cutaneous T-Cell Lymphoma. Neoplasia 21:82-92
Denu, Ryan A; Shabbir, Maria; Nihal, Minakshi et al. (2018) Centriole Overduplication is the Predominant Mechanism Leading to Centrosome Amplification in Melanoma. Mol Cancer Res 16:517-527

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