Abstract

The Histotechnology Laboratory, under the supervision of Dr. Henry C. Pitot is located in four modules (approximately 88 square feet of space in the basement of the McArdle Laboratory. A total of five research specialists and one laboratory technician work full or part-time in the facility as does a half-time student laboratory assistant. The Laboratory is equipped with two chemical fume hoods, two cytostats, two automated tissue processors, four Leitz microtomes, Tissue Tek Embedding Center, microtome knife sharpener, Pope Solvent Recovery System, a freezer, refrigerator, and other small equipment necessary for the operation of the facility. The Histotechnology Laboratory is equipped to perform a wide range of services including the preparation of histological sections from both paraffin-embedded and cryostat material, histochemical staining (e.g., gamma-glutamyltranspeptidase, glucose-6-phosphatase, ATPase), special stains (such as PAS, Giemsa, Iron) and special procedures (autoradiography, immunohistochemistry, block preparations of cell pellets or embryoid bodies, in situ staining, and histochemistry and autoradiography of cell cultures.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA007175-36
Application #
6299905
Study Section
Project Start
2000-04-01
Project End
2001-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
36
Fiscal Year
2000
Total Cost
$216,384
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Thompson, Nancy E; Glaser, Bryan T; Foley, Katherine M et al. (2009) Minimal promoter systems reveal the importance of conserved residues in the B-finger of human transcription factor IIB. J Biol Chem 284:24754-66
Habig, Jeffrey W; Loeb, Daniel D (2006) Sequence identity of the direct repeats, DR1 and DR2, contributes to the discrimination between primer translocation and in situ priming during replication of the duck hepatitis B virus. J Mol Biol 364:32-43
Liu, Ning; Ji, Lin; Maguire, Megan L et al. (2004) cis-Acting sequences that contribute to the synthesis of relaxed-circular DNA of human hepatitis B virus. J Virol 78:642-9
Tessier, Charles R; Doyle, Glenn A; Clark, Brad A et al. (2004) Mammary tumor induction in transgenic mice expressing an RNA-binding protein. Cancer Res 64:209-14
Ostrow, Kristin M; Loeb, Daniel D (2004) Underrepresentation of the 3' region of the capsid pregenomic RNA of duck hepatitis B virus. J Virol 78:2179-86
Habig, Jeffrey W; Loeb, Daniel D (2003) Template switches during plus-strand DNA synthesis of duck hepatitis B virus are influenced by the base composition of the minus-strand terminal redundancy. J Virol 77:12412-20
Habig, Jeffrey W; Loeb, Daniel D (2003) The conformation of the 3' end of the minus-strand DNA makes multiple contributions to template switches during plus-strand DNA synthesis of duck hepatitis B virus. J Virol 77:12401-11
Bunger, Maureen K; Moran, Susan M; Glover, Edward et al. (2003) Resistance to 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity and abnormal liver development in mice carrying a mutation in the nuclear localization sequence of the aryl hydrocarbon receptor. J Biol Chem 278:17767-74
Liu, Ning; Tian, Ru; Loeb, Daniel D (2003) Base pairing among three cis-acting sequences contributes to template switching during hepadnavirus reverse transcription. Proc Natl Acad Sci U S A 100:1984-9
Mueller-Hill, Karlyn; Loeb, Daniel D (2002) cis-Acting sequences 5E, M, and 3E interact to contribute to primer translocation and circularization during reverse transcription of avian hepadnavirus DNA. J Virol 76:4260-6

Showing the most recent 10 out of 79 publications