The Flow Cytometry Core at MSKCC provides researchers at MSKCC with state of the art flow cytometry services, including cell sorting. These services include operator-assisted flow cytometry analysis on a CyAn and a FACSCaliburflow cytometer, self-operated flow analysis on two 5-color (upgraded) FACScans and a FACSCalibur, and operator-assisted cell sorting on one 4-laser MoFlo, one 3-laser MoFlo, and one 3-laser DiVa cell sorter. Extensive training in flow cytometry is offered, and a site license for Flowjo is administered by the core facility. An additional cell purification option, Miltenyi AutoMACS, is made available to all users. The availability of these services enables the researchers at MSKCC to isolate and characterize specific cellular subsets, and to analyze the effects of manipulations aimed at answering a multitude of scientific questions that arise within the context of cancer research. The core facility's services play in important role in enabling the researchers to ask specific questions regarding ever smaller subsets of cells, down to the single cell level. Many of these questions can only be answered when purified fractions of specific cell subsets can be identified and isolated, which is the domain of flow analysis and cell sorting.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Sloan-Kettering Institute for Cancer Research
New York
United States
Zip Code
Orlow, I; Satagopan, J M; Berwick, M et al. (2015) Genetic factors associated with naevus count and dermoscopic patterns: preliminary results from the Study of Nevi in Children (SONIC). Br J Dermatol 172:1081-9
Carey, Bryce W; Finley, Lydia W S; Cross, Justin R et al. (2015) Intracellular ?-ketoglutarate maintains the pluripotency of embryonic stem cells. Nature 518:413-6
Mosher, C E; Given, B A; Ostroff, J S (2015) Barriers to mental health service use among distressed family caregivers of lung cancer patients. Eur J Cancer Care (Engl) 24:50-9
Navi, Babak B; Reiner, Anne S; Kamel, Hooman et al. (2015) Association between incident cancer and subsequent stroke. Ann Neurol 77:291-300
Xu, Zhe; Wu, Chaochao; Xie, Fang et al. (2015) Comprehensive quantitative analysis of ovarian and breast cancer tumor peptidomes. J Proteome Res 14:422-33
Xu, Hong; Cheng, Ming; Guo, Hongfen et al. (2015) Retargeting T cells to GD2 pentasaccharide on human tumors using Bispecific humanized antibody. Cancer Immunol Res 3:266-77
Gondo, Tatsuo; Poon, Bing Ying; Matsumoto, Kazuhiro et al. (2015) Clinical role of pathological downgrading after radical prostatectomy in patients with biopsy confirmed Gleason score 3 + 4 prostate cancer. BJU Int 115:81-6
Kaittanis, Charalambos; Shaffer, Travis M; Thorek, Daniel L J et al. (2014) Dawn of advanced molecular medicine: nanotechnological advancements in cancer imaging and therapy. Crit Rev Oncog 19:143-76
Das, Sudeep; Thorek, Daniel L J; Grimm, Jan (2014) Cerenkov imaging. Adv Cancer Res 124:213-34
Fay, Allison; Glickman, Michael S (2014) An essential nonredundant role for mycobacterial DnaK in native protein folding. PLoS Genet 10:e1004516

Showing the most recent 10 out of 836 publications