The Molecular Cytology Core Facility (MCCF) underpins all the basic and clinical research at MSKCC involving the interrogation of proteins or nucleic acids in cells, tissues and tumors. The MCCF retains state-of- the-art equipment and an array of imaging tools and a team of highly talented technical assistants and as such provides both a service and one-on-one training enabling faculty and their trainees to work independently with high-end instrumentation. A resource of validated antibodies for automated or manual histology is a feature of the Core's rapid and reliable service used by investigators working with animal tumor models or patient samples. To explore the cell biology of cancer cells at high spatial and temporal resolution, the Core's in-house expertise enables the power of a superb suite of confocal microscopes to be accessible not only to cell biologists but also to the wider community of members of the Cancer Center. Imaging modalities for molecular detection available to the researchers at the MCCF are optical microscopes, including wide field (epifluorescence, bright field, polarizing and DIC), confocal (raster scanning, line scanning and spinning disc), time lapse microscopy, FLIM and digital scanners. Experiments involving uncaging experiments, FRAP and FRET, and Ratio imaging of calcium ions are also carried out at the MCCF. In addition to assistance and training on image acquisition, the MCCF staff provide assistance to researchers in image processing and analysis using Velocity, MetaMorph, Imaris and MatLab software. The broad range of services and collaborative work provided by the Molecular Cytology Core has supported the research of 120 investigators in the past year. During the past grant period the work of the Core has contributed to 315 publications of researchers from 5 research programs. For example, with the assistance of the Core, Studer and Tabar demonstrated that neural rosette cells represent the first characterized neural stem cell stage capable of responding to patterning cues that direct differentiation toward region-specific neuronal fates. The Core provided immunohistochemistry and image analysis critical to this work.

Public Health Relevance

The Molecular Cytology Core provides cutting edge in situ detection and optical imaging platforms. As our collective understanding of cancer genetics increases there will be an increasing need to understand the function and behavior of key drivers;moreover, it is becoming increasingly apparent that cancers are heterogeneous and studying molecular function at the level of individual cells in tissues will be important for understanding the biological and clinical impact of such heterogeneity.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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Shafik, Hasnaa
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Sloan-Kettering Institute for Cancer Research
New York
United States
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Steuer, Conor E; Behera, Madhusmita; Berry, Lynne et al. (2016) Role of race in oncogenic driver prevalence and outcomes in lung adenocarcinoma: Results from the Lung Cancer Mutation Consortium. Cancer 122:766-72
Dominguez-Rosado, Ismael; Moutinho Jr, Vitor; DeMatteo, Ronald P et al. (2016) Outcomes of the Memorial Sloan Kettering Cancer Center International General Surgical Oncology Fellowship. J Am Coll Surg 222:961-6
Iasonos, Alexia; O'Quigley, John (2016) Integrating the escalation and dose expansion studies into a unified Phase I clinical trial. Contemp Clin Trials 50:124-34
Ulaner, Gary A; Hyman, David M; Ross, Dara S et al. (2016) Detection of HER2-Positive Metastases in Patients with HER2-Negative Primary Breast Cancer Using 89Zr-Trastuzumab PET/CT. J Nucl Med 57:1523-1528
Brown, Anna M; Nagala, Sidhartha; McLean, Mary A et al. (2016) Multi-institutional validation of a novel textural analysis tool for preoperative stratification of suspected thyroid tumors on diffusion-weighted MRI. Magn Reson Med 75:1708-16
Akkari, Leila; Gocheva, Vasilena; Quick, Marsha L et al. (2016) Combined deletion of cathepsin protease family members reveals compensatory mechanisms in cancer. Genes Dev 30:220-32
Theilen, Till M; Chou, Alexander J; Klimstra, David S et al. (2016) Esophageal Adenocarcinoma and Squamous Cell Carcinoma in Children and Adolescents: Report of 3 Cases and Comprehensive Literature Review. J Pediatr Surg Case Rep 5:23-29
Robinson, June K; Halpern, Allan C (2016) Cost-effective Melanoma Screening. JAMA Dermatol 152:19-21
Calzavara-Pinton, Pier Giacomo; Rossi, Maria Teresa; Zanca, Arianna et al. (2016) Oral Polypodium leucomotos increases the anti-inflammatory and melanogenic responses of the skin to different modalities of sun exposures: a pilot study. Photodermatol Photoimmunol Photomed 32:22-7
Ripley, R Taylor; Suzuki, Kei; Tan, Kay See et al. (2016) Postinduction positron emission tomography assessment of N2 nodes is not associated with ypN2 disease or overall survival in stage IIIA non-small cell lung cancer. J Thorac Cardiovasc Surg 151:969-77, 979.e1-3

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