The Molecular Cytology Core Facility (MCCF) underpins all the basic and clinical research at MSKCC involving the interrogation of proteins or nucleic acids in cells, tissues and tumors. The MCCF retains state-of- the-art equipment and an array of imaging tools and a team of highly talented technical assistants and as such provides both a service and one-on-one training enabling faculty and their trainees to work independently with high-end instrumentation. A resource of validated antibodies for automated or manual histology is a feature of the Core's rapid and reliable service used by investigators working with animal tumor models or patient samples. To explore the cell biology of cancer cells at high spatial and temporal resolution, the Core's in-house expertise enables the power of a superb suite of confocal microscopes to be accessible not only to cell biologists but also to the wider community of members of the Cancer Center. Imaging modalities for molecular detection available to the researchers at the MCCF are optical microscopes, including wide field (epifluorescence, bright field, polarizing and DIC), confocal (raster scanning, line scanning and spinning disc), time lapse microscopy, FLIM and digital scanners. Experiments involving uncaging experiments, FRAP and FRET, and Ratio imaging of calcium ions are also carried out at the MCCF. In addition to assistance and training on image acquisition, the MCCF staff provide assistance to researchers in image processing and analysis using Velocity, MetaMorph, Imaris and MatLab software. The broad range of services and collaborative work provided by the Molecular Cytology Core has supported the research of 120 investigators in the past year. During the past grant period the work of the Core has contributed to 315 publications of researchers from 5 research programs. For example, with the assistance of the Core, Studer and Tabar demonstrated that neural rosette cells represent the first characterized neural stem cell stage capable of responding to patterning cues that direct differentiation toward region-specific neuronal fates. The Core provided immunohistochemistry and image analysis critical to this work.

Public Health Relevance

The Molecular Cytology Core provides cutting edge in situ detection and optical imaging platforms. As our collective understanding of cancer genetics increases there will be an increasing need to understand the function and behavior of key drivers;moreover, it is becoming increasingly apparent that cancers are heterogeneous and studying molecular function at the level of individual cells in tissues will be important for understanding the biological and clinical impact of such heterogeneity.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA008748-48
Application #
8933517
Study Section
Subcommittee G - Education (NCI)
Program Officer
Shafik, Hasnaa
Project Start
2014-01-01
Project End
2018-12-31
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
48
Fiscal Year
2014
Total Cost
$460,563
Indirect Cost
$201,383
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Pak, Linda M; Gonen, Mithat; Seier, Kenneth et al. (2018) Can physician gestalt predict survival in patients with resectable pancreatic adenocarcinoma? Abdom Radiol (NY) 43:2113-2118
Thomas, Rozario; Marks, Daniel Henry; Chin, Yvette et al. (2018) Whole chromosome loss and associated breakage-fusion-bridge cycles transform mouse tetraploid cells. EMBO J 37:201-218
Shoag, Jonathan; Liu, Deli; Blattner, Mirjam et al. (2018) SPOP mutation drives prostate neoplasia without stabilizing oncogenic transcription factor ERG. J Clin Invest 128:381-386
Abreu, Carla M; Prakash, Rohit; Romanienko, Peter J et al. (2018) Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination. Nat Commun 9:3961
Katsoulakis, Evangelia; Leeman, Jonathan E; Lok, Benjamin H et al. (2018) Long-term outcomes in oral cavity squamous cell carcinoma with adjuvant and salvage radiotherapy after surgery. Laryngoscope 128:2539-2545
Huicochea Castellanos, Sandra; Corrias, Giuseppe; Ulaner, Gary A et al. (2018) Detection of recurrent pancreatic cancer: value of second-opinion interpretations of cross-sectional images by subspecialized radiologists. Abdom Radiol (NY) :
Hoseini, Sayed Shahabuddin; Guo, Hongfen; Wu, Zhihao et al. (2018) A potent tetravalent T-cell-engaging bispecific antibody against CD33 in acute myeloid leukemia. Blood Adv 2:1250-1258
Attiyeh, Marc A; Fernández-Del Castillo, Carlos; Al Efishat, Mohammad et al. (2018) Development and Validation of a Multi-institutional Preoperative Nomogram for Predicting Grade of Dysplasia in Intraductal Papillary Mucinous Neoplasms (IPMNs) of the Pancreas: A Report from The Pancreatic Surgery Consortium. Ann Surg 267:157-163
Tollinche, Luis; Tan, KaySee; Han, Austin et al. (2018) Analyzing Volatile Anesthetic Consumption by Auditing Fresh Gas Flow: An Observational Study at an Academic Hospital. Int J Anesth Anesth 5:
Tadros, Audree B; Wen, Hannah Y; Morrow, Monica (2018) Breast Cancers of Special Histologic Subtypes Are Biologically Diverse. Ann Surg Oncol 25:3158-3164

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