The Gene Transfer and Somatic Cell Engineering Core (GTF) supports the preclinical translation and clinical implementation of gene transfer studies at MSKCC. The projects supported are highly dependent on achieving efficient gene transfer in primary cells, including hematopoietic progenitor cells, T lymphocytes and dendritic cells. In the upcoming grant cycle, the GTF will focus on the implementation of Phase l/ll clinical trials and on the development and optimization of clinical cell engineering processes. The specific alms of the GTF are to carry out: 1) Expansion and transduction of patient cells for clinical trials utilizing expanded and/or genetically modified cells;2) Generation and characterization of high-titer producer cell clones, master cell banks (MCB);3) Production and titration of 5 to 15 liter batches of clinical viral stocks in semi-closed systems;4) Biosafety testing in cultured packaging cell clones (MCB), viral stocks and clinical specimen;5) Monitoring of retroviral vector copy number and integration sites by LAM-PCR in patient cells; and 6) Cell banking, storage of viral stocks, plasmid DNA and clinical specimens. In addition, the GTF provides essential advisory and training functions for generation of research grade reagents. The GTF is a repository for numerous reagents and protocols that are made available to investigators at MSKCC. The centralization of cell transdudion, vector production and plasmid DNA manufacturing in the GTF decreases the cost of clinical development. It also ensures high quality, consistency and timely implementation of molecular and cellular processes, and their availability to all investigators at the Center both at the preclinical and clinical stages. Over the last funding period the GTF enabled the successful implementation of 7 clinical trials currently open for enrollment under 4 investigational new drug applications. The services of the GTF supported the research of 11 investigators in the past year. During the past grant period the Core contributed to 107 publications of researchers from 3 programs.

Public Health Relevance

The GTF investigates issues facing adoptive cell therapies and supports the translation of the most promising approaches in clinical trials designed to enroll subjects with advanced, refractory cancers.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA008748-48
Application #
8933535
Study Section
Subcommittee G - Education (NCI)
Program Officer
Shafik, Hasnaa
Project Start
2014-01-01
Project End
2018-12-31
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
48
Fiscal Year
2014
Total Cost
$369,893
Indirect Cost
$161,737
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
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Bhagat, Tushar D; Zou, Yiyu; Huang, Shizheng et al. (2017) Notch Pathway Is Activated via Genetic and Epigenetic Alterations and Is a Therapeutic Target in Clear Cell Renal Cancer. J Biol Chem 292:837-846
Prieto-Granada, Carlos N; Zhang, Lei; Antonescu, Cristina R et al. (2017) Primary cutaneous adenoid cystic carcinoma with MYB aberrations: report of three cases and comprehensive review of the literature. J Cutan Pathol 44:201-209
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