The primary role of the Mouse Genetics Core Facility (MGCP) Is to facilitate the use of mouse molecular genetics at MSKCC for in vivo studies of gene functions germane to cancer. Relevant fields where mouse models can be applied include cell growth and behavior, cellular differentiation, embryonic development, immunobiology, genome integrity, and malignant transformation. The MGCP consists of 3 groups: the Transgenic Mouse Group, the Colony Management Group, and the ES Cell Culture Group. Some of the services these groups provide are: (1) Production of transgenic mice: transgene DNA purification, pronuclear injection, genotyping of founder mice, and breeding of positive founders to provide Gl progeny. (2) Gene Targeting: electroporation of gene targeting vector into ES cells, selection and identification of clones, and cryopreservation of targeted ES cell clones. (3) Production of gene targeted mice: generation of chimeric mice by blastocyst Injection and identification of germline chimeras. (4) Long term storage of mouse strains by cryopreservation of sperm or embryos. (5) Rederivation by embryo transfer or IVF for strain importation and recovery. (6) Performance of specialized animal surgical procedures and embryological techniques. (7) Provision of specialized mouse strains/lines for investigators' research. (8) Comprehensive management of research animal colonies for investigators. (9) Genotyping of transgenic, gene targeted, and mutant mouse lines. The services provided by the Mouse Genetics Core has supported the research of 74 investigators in the past year. During the past grant period the work of the Core has contributed to 241 publications of researchers from 8 research programs. For example, the Core provided services to study the relationship between genome integrity and cancer. The Core carried out ES cell injections for the derivation of novel mouse strains in which certain facets of the DNA damage response are impaired by mutation affecting the Mre11 complex, the apical sensor of DNA damage mammals. Collectively, the data obtained led to the publication of work that described the central role of the Mre11 complex in tumor suppression by the DNA damage response.

Public Health Relevance

The MGCP provides a comprehensive and interconnected set of services that are essential to the production, management and preservation of genetically modified mice at MSKCC. These services enable Center investigators to establish animal models suitable for studies on a wide range of problems relevant to cancer biology, such as the genetic basis of oncogenesis and metastasis, as well as problems applicable to therapeutics such as the evaluation of new drugs and drug targets.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA008748-49
Application #
8933674
Study Section
Subcommittee G - Education (NCI)
Program Officer
Shafik, Hasnaa
Project Start
Project End
Budget Start
2015-01-01
Budget End
2015-12-31
Support Year
49
Fiscal Year
2015
Total Cost
$811,620
Indirect Cost
$354,885
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Lichtenthal, Wendy G; Maciejewski, Paul K; Craig Demirjian, Caraline et al. (2018) Evidence of the clinical utility of a prolonged grief disorder diagnosis. World Psychiatry 17:364-365
Cottrell, T R; Thompson, E D; Forde, P M et al. (2018) Pathologic features of response to neoadjuvant anti-PD-1 in resected non-small-cell lung carcinoma: a proposal for quantitative immune-related pathologic response criteria (irPRC). Ann Oncol 29:1853-1860
Pereira, PatrĂ­cia M R; Sharma, Sai Kiran; Carter, Lukas M et al. (2018) Caveolin-1 mediates cellular distribution of HER2 and affects trastuzumab binding and therapeutic efficacy. Nat Commun 9:5137
Mano, Roy; Di Natale, Renzo; Sheinfeld, Joel (2018) Current controversies on the role of retroperitoneal lymphadenectomy for testicular cancer. Urol Oncol :
Zhu, Guo; Benayed, Ryma; Ho, Caleb et al. (2018) Diagnosis of known sarcoma fusions and novel fusion partners by targeted RNA sequencing with identification of a recurrent ACTB-FOSB fusion in pseudomyogenic hemangioendothelioma. Mod Pathol :
Gollub, Marc J; Hotker, Andreas M; Woo, Kaitlin M et al. (2018) Quantitating whole lesion tumor biology in rectal cancer MRI: taking a lesson from FDG-PET tumor metrics. Abdom Radiol (NY) 43:1575-1582
Rapp, Moritz; Wiedemann, Gabriela M; Sun, Joseph C (2018) Memory responses of innate lymphocytes and parallels with T cells. Semin Immunopathol 40:343-355
Rutter, Carolyn M; Kim, Jane J; Meester, Reinier G S et al. (2018) Effect of Time to Diagnostic Testing for Breast, Cervical, and Colorectal Cancer Screening Abnormalities on Screening Efficacy: A Modeling Study. Cancer Epidemiol Biomarkers Prev 27:158-164
Zabor, Emily C; Furberg, Helena; Lee, Byron et al. (2018) Long-Term Renal Function Recovery following Radical Nephrectomy for Kidney Cancer: Results from a Multicenter Confirmatory Study. J Urol 199:921-926
Bakhoum, Samuel F; Ngo, Bryan; Laughney, Ashley M et al. (2018) Chromosomal instability drives metastasis through a cytosolic DNA response. Nature 553:467-472

Showing the most recent 10 out of 8799 publications