The primary goal of the X-ray Crystallography Core Facility is to provide access to state-of-the-art hardware, software and expertise to Cancer Center laboratories that use X-ray crystallography as a tool to address questions in their research programs. The Core provides and maintains In-house equipment and computational resources for data collection, processing and structure determination, implements a wide range of crystallographic and structure analysis software packages, participates in multi-institutional consortia for long-term regular access to synchrotron beamlines, and provides training and technical assistance to users of both the in-house and remote facilities. In addition, the Core Head collaborates with non-structural MSKCC groups on macromolecular crystallization and structure-determination projects, and also provides modeling expertise and guidance to those laboratories that can benefit from the use of available structures in the design and interpretation of experiments. Understanding the biological processes involved in tumorigenesis has increasingly benefitted from structural investigations that provide key functional and mechanistic Insights, as well as atomic-level details important for drug discovery. Many of the questions require the structure determination of large proteins, assemblies and macromolecular machines. Advances in protein expression technologies and nano-liter crystallization robots are making it possible to obtain hitherto inaccessible crystals. However, crystals of such complexes often present major experimental challenges due to small crystal size, large unit cells, poor order and limited diffraction. The X-ray Core alms to help address these limitations for MSKCC investigators by providing the latest generation in-house X-ray diffraction systems, by testing and implementing new methods, tools and software and disseminating the latest improvements, and by participating in multi-institutional consortia that not only build and operate synchrotron beamlines, but also carry out technology research and development that push the envelope of beamline capabilities. The X-Ray Crystallography Core has supported 8 investigators in the past year. During the past grant period the Core has contributed to 115 publications of researchers from 3 programs.

Public Health Relevance

Understanding the biological processes involved in tumorigenesis requires the understanding of the underlying structures of relevant proteins, genes and enzymes. The X-Ray Crystallography core provides services to MSKCC investigators interested in structure-function determinations.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA008748-51
Application #
9204764
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2017-01-01
Budget End
2017-12-31
Support Year
51
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Meyer, Jan-Philip; Tully, Kathryn M; Jackson, James et al. (2018) Bioorthogonal Masking of Circulating Antibody-TCO Groups Using Tetrazine-Functionalized Dextran Polymers. Bioconjug Chem 29:538-545
McFarland, Daniel C; Shaffer, Kelly; Breitbart, William et al. (2018) C-reactive protein and its association with depression in patients receiving treatment for metastatic lung cancer. Cancer :
Lacouture, Mario E; Anadkat, Milan; Jatoi, Aminah et al. (2018) Dermatologic Toxicity Occurring During Anti-EGFR Monoclonal Inhibitor Therapy in Patients With Metastatic Colorectal Cancer: A Systematic Review. Clin Colorectal Cancer 17:85-96
Blumenthal, Gideon M; Bunn Jr, Paul A; Chaft, Jamie E et al. (2018) Current Status and Future Perspectives on Neoadjuvant Therapy in Lung Cancer. J Thorac Oncol 13:1818-1831
Shaffer, Kelly M; Nelson, Christian J; DuHamel, Katherine N (2018) Barriers to participation in a sexual health intervention for men following treatment for rectal and anal cancer. Psychooncology 27:1082-1085
Park, Kay J; Patel, Prusha; Linkov, Irina et al. (2018) Observations on the origin of ovarian cortical inclusion cysts in women undergoing risk-reducing salpingo-oophorectomy. Histopathology 72:766-776
Pianko, Matthew J; Goldberg, Aaron D; Lesokhin, Alexander M (2018) Clinical Development of PD-1 Blockade in Hematologic Malignancies. Cancer J 24:31-35
Navi, Babak B; Marshall, Randolph S; Bobrow, Dylan et al. (2018) Enoxaparin vs Aspirin in Patients With Cancer and Ischemic Stroke: The TEACH Pilot Randomized Clinical Trial. JAMA Neurol 75:379-381
Shah, Sona; Boucai, Laura (2018) Effect of Age on Response to Therapy and Mortality in Patients With Thyroid Cancer at High Risk of Recurrence. J Clin Endocrinol Metab 103:689-697
Goldman, Debra A; Hovinga, Koos; Reiner, Anne S et al. (2018) The relationship between repeat resection and overall survival in patients with glioblastoma: a time-dependent analysis. J Neurosurg :1-9

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