The Microchemistry and Proteomics Core Facility (MPCF) supports laboratory and clinical research programs at MSKCC by 1) providing synthetic peptides and by 2) carrying out mass spectrometric and amino acid sequence analyses of proteins and peptides, generally for the purpose of identification, relative quantitative analysis and post-translational modification (PTM) analysis. Synthetic molecules are made according to specifications provided by the investigator. Polypeptides submitted for identification (either single proteins or mixtures), PTM analysis, quantitation or N-terminal sequencing are prepared by the lab requesting service. Information on chemistries and instrumentation, and expert advice on experimental approaches are provided by Core staff. The goal of proteomics is to analyze changing levels, local concentrations and post-translational modifications of proteins in cells, as well as to analyze higher-order networks of protein-protein interactions. Proteins and protein modifications are critical in the complex signaling pathways and regulatory processes underlying cell growth, division, differentiation, DNA repair, development, senescence, and in responses to bioactive agents, genetic alterations and disease. Dissection of multi-component protein complexes is therefore increasingly the focus of studies on molecular control mechanisms. Identification of unique components provides a powerful approach to the selective retrieval and identification of the companions. The impact that these services have provided, in facilitating research projects and enabling the timely progression of high priority research, can be recognized throughout the reports of the several research programs. In a multi-institutional study led by MSKCC, affinity-based proteomics was used to examine the cancer-specific networks that are coordinated by Hsp90. These studies showed that the HSP90 inhibitor PU0H71, developed by Dr. Chiosis (ET), preferentially targets tumor enriched Hsp90 complexes and can be used to affinity capture Hsp90-dependent oncogenic client proteins. The Core has supported the research of 46 investigators during the past year. Over the past grant period, investigators from 9 programs were supported and the Core has contributed to 168 publications.

Public Health Relevance

The MPCF is a biomedical proteomics center that provides essential support to laboratory and clinical research programs at MSKCC by performing mass spectrometry-based sequence analysis of proteins and peptides, generally for the purpose of identification, relative quantitative analysis and characterization of post translational modifications.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA008748-51
Application #
9204779
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2017-01-01
Budget End
2017-12-31
Support Year
51
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Pereira, PatrĂ­cia M R; Sharma, Sai Kiran; Carter, Lukas M et al. (2018) Caveolin-1 mediates cellular distribution of HER2 and affects trastuzumab binding and therapeutic efficacy. Nat Commun 9:5137
Mano, Roy; Di Natale, Renzo; Sheinfeld, Joel (2018) Current controversies on the role of retroperitoneal lymphadenectomy for testicular cancer. Urol Oncol :
Zhu, Guo; Benayed, Ryma; Ho, Caleb et al. (2018) Diagnosis of known sarcoma fusions and novel fusion partners by targeted RNA sequencing with identification of a recurrent ACTB-FOSB fusion in pseudomyogenic hemangioendothelioma. Mod Pathol :
Gollub, Marc J; Hotker, Andreas M; Woo, Kaitlin M et al. (2018) Quantitating whole lesion tumor biology in rectal cancer MRI: taking a lesson from FDG-PET tumor metrics. Abdom Radiol (NY) 43:1575-1582
Rapp, Moritz; Wiedemann, Gabriela M; Sun, Joseph C (2018) Memory responses of innate lymphocytes and parallels with T cells. Semin Immunopathol 40:343-355
Rutter, Carolyn M; Kim, Jane J; Meester, Reinier G S et al. (2018) Effect of Time to Diagnostic Testing for Breast, Cervical, and Colorectal Cancer Screening Abnormalities on Screening Efficacy: A Modeling Study. Cancer Epidemiol Biomarkers Prev 27:158-164
Zabor, Emily C; Furberg, Helena; Lee, Byron et al. (2018) Long-Term Renal Function Recovery following Radical Nephrectomy for Kidney Cancer: Results from a Multicenter Confirmatory Study. J Urol 199:921-926
Bakhoum, Samuel F; Ngo, Bryan; Laughney, Ashley M et al. (2018) Chromosomal instability drives metastasis through a cytosolic DNA response. Nature 553:467-472
Pilewskie, Melissa; Morrow, Monica (2018) Margins in breast cancer: How much is enough? Cancer 124:1335-1341
Leng, Jennifer C; Lei, Lei; Lei, Shu Fang et al. (2018) Smoking Among Chinese Livery Drivers. J Immigr Minor Health :

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