The Wistar Institute provides an AAALAC-accredited Animal Facility that serves faculty from the Gene Expression and Regulation, Oncogenesis, and Immunology Cancer Center programs. The facility is maintained as a modified barrier Biosafety Level 2 (BSL2) facility by highly qualified and experienced personnel. In addition to ordering and housing research animals, the facility houses the Mouse Genetics Facility. The Animal Facility supports the severe combined immunodeficiency (SCID) mouse breeding colony, quarantine housing, technical training, Intravital Imaging System (MS) 200i in vivo imaging capabilities, animal health surveillance, veterinary coverage, new employee hands-on training, and Institutional Animal Care and Use Committee (IACUC) testing. Since 2003, The Wistar Institute has invested over $1 million into the Animal Facility for structural upgrades and new equipment supporting Cancer Center programs. A new polishing chiller augments chilled water supplies for cooling, and ensures stable temperature regulation for the Facility during the hottest weather, as well as back-up chilled water supplies in the event of failure in the primary cooling system. Additional upgrades to the facility infrastructure include improved ventilation, security, and emergency capabilities. Direct Digital Control monitoring has been incorporated into all animal rooms, offering 24-hour monitoring of the temperature as well as the heating, ventilation, and air condition (HVAC) system. Research infrastructure improvements include the addition of an IVIS imager, permitting tumor growth monitoring over time at high resolution;new state-of-the-art biocontainment equipment for expanding studies involving viral vectors and other infectious agents;and a transgenic mouse suite for use by the expanding Mouse Genetics Shared Facility. Future goals reflect the needs of cancer researchers and shared facilities and include the purchase of additional bio-containment caging equipment and Class II bio-safety cabinets;an anesthesia machine;a stereomicroscope for mouse surgical procedures;and continued upgrades to security systems.

Public Health Relevance

Animal models of human cancer have become irreplaceable tools to study how cancer behaves in the context of development, microenvironment, immunology, angiogenesis, progression, invasion and metastasis. These studies are only valid if the animals are cared for in a controlled, humane environment. The Animal Facility is run by a very professional staff that ensures these principles.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA010815-44
Application #
8461259
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
44
Fiscal Year
2013
Total Cost
$222,586
Indirect Cost
$93,178
Name
Wistar Institute
Department
Type
DUNS #
075524595
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Seo, Jae Ho; Rivadeneira, Dayana B; Caino, M Cecilia et al. (2016) The Mitochondrial Unfoldase-Peptidase Complex ClpXP Controls Bioenergetics Stress and Metastasis. PLoS Biol 14:e1002507
Haut, Larissa H; Gill, Amanda L; Kurupati, Raj K et al. (2016) A Partial E3 Deletion in Replication-Defective Adenoviral Vectors Allows for Stable Expression of Potentially Toxic Transgene Products. Hum Gene Ther Methods :
Peck, Barrie; Schug, Zachary T; Zhang, Qifeng et al. (2016) Inhibition of fatty acid desaturation is detrimental to cancer cell survival in metabolically compromised environments. Cancer Metab 4:6
Tempera, Italo; De Leo, Alessandra; Kossenkov, Andrew V et al. (2016) Identification of MEF2B, EBF1, and IL6R as Direct Gene Targets of Epstein-Barr Virus (EBV) Nuclear Antigen 1 Critical for EBV-Infected B-Lymphocyte Survival. J Virol 90:345-55
Nelson, David M; Jaber-Hijazi, Farah; Cole, John J et al. (2016) Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol 17:158
Kumar, Vinit; Patel, Sima; Tcyganov, Evgenii et al. (2016) The Nature of Myeloid-Derived Suppressor Cells in the Tumor Microenvironment. Trends Immunol 37:208-20
Kung, Che-Pei; Murphy, Maureen E (2016) The role of the p53 tumor suppressor in metabolism and diabetes. J Endocrinol 231:R61-R75
Patro, Sean C; Azzoni, Livio; Joseph, Jocelin et al. (2016) Antiretroviral therapy in HIV-1-infected individuals with CD4 count below 100 cells/mm3 results in differential recovery of monocyte activation. J Leukoc Biol 100:223-31
Chae, Young Chan; Vaira, Valentina; Caino, M Cecilia et al. (2016) Mitochondrial Akt Regulation of Hypoxic Tumor Reprogramming. Cancer Cell 30:257-72
Vazquez, Alexei; Kamphorst, Jurre J; Markert, Elke K et al. (2016) Cancer metabolism at a glance. J Cell Sci 129:3367-73

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