The imaging of complex cellular structures is central to modern cancer biology, and a state-of-the-art Microscopy Facility is essential to the mission of a Cancer Center. As such, the Wistar Institute has made a strong effort to acquire the necessary instrumentation to accomplish its mission and to have it managed by a highly competent biological imaging specialist, Mr. James Hayden. The Microscopy Facility has served an important role for the members of the Wistar Cancer Center since 1973. Over the past five years, the Facility has grown tremendously and, with changes in scientific focus and the addition of new technologies and instrumentation, has evolved into a premier asset of the Institute and Cancer Center. With new leadership, a reconfiguration of space that improved utilization, new technical applications, new support services, and training at all levels, the Facility has experienced a substantial increase in Cancer Center member usage. Since 2003, thirty-one out of 32 laboratories from all three Cancer Center research programs have used services provided by the Microscopy Facility. Significant scientific accomplishments published in high impact journals have been achieved using new facility equipment, including the Xenogen MS imaging system for in vivo bioluminescent studies of tumor metastases (Huang, Kissil and Pure), the 2 Photon microscopy system to image in vivo movement of immune cells and their interaction with tumor cells (Ertl and Weninger laboratories), and the Live-Cell microscopy system to image in-vitro cell-cell and cell-matrix interactions (M. Herlyn, Heber-Katz, and Pure laboratories). Standard wide-field and confocal microscopy (Lieberman, Maul and Rauscher laboratories) available through the Facility has also aided studies in molecular interactions at the subcellular level. Since the previous renewal, approximately $514,000 was committed by the Institute for new instrumentation and upgrades.
Without the ability to provide expensive high end microscopy instruments, instruction, and support to all Cancer Center members, they would not be able to perform many experiments without purchasing this equipment in their individual labs and this would delay valuable cancer research.
|Fukumoto, Takeshi; Park, Pyoung Hwa; Wu, Shuai et al. (2018) Repurposing Pan-HDAC Inhibitors for ARID1A-Mutated Ovarian Cancer. Cell Rep 22:3393-3400|
|Bhattacharjee, Souvik; Coppens, Isabelle; Mbengue, Alassane et al. (2018) Remodeling of the malaria parasite and host human red cell by vesicle amplification that induces artemisinin resistance. Blood 131:1234-1247|
|Lu, Yunqi; Hu, Zhongyi; Mangala, Lingegowda S et al. (2018) MYC Targeted Long Noncoding RNA DANCR Promotes Cancer in Part by Reducing p21 Levels. Cancer Res 78:64-74|
|Thangavel, Chellappagounder; Boopathi, Ettickan; Liu, Yi et al. (2018) Therapeutic Challenge with a CDK 4/6 Inhibitor Induces an RB-Dependent SMAC-Mediated Apoptotic Response in Non-Small Cell Lung Cancer. Clin Cancer Res 24:1402-1414|
|Duperret, Elizabeth K; Liu, Shujing; Paik, Megan et al. (2018) A Designer Cross-reactive DNA Immunotherapeutic Vaccine that Targets Multiple MAGE-A Family Members Simultaneously for Cancer Therapy. Clin Cancer Res 24:6015-6027|
|Duperret, Elizabeth K; Wise, Megan C; Trautz, Aspen et al. (2018) Synergy of Immune Checkpoint Blockade with a Novel Synthetic Consensus DNA Vaccine Targeting TERT. Mol Ther 26:435-445|
|Peng, Hongzhuang; Prokop, Jeremy; Karar, Jayashree et al. (2018) Familial and Somatic BAP1 Mutations Inactivate ASXL1/2-Mediated Allosteric Regulation of BAP1 Deubiquitinase by Targeting Multiple Independent Domains. Cancer Res 78:1200-1213|
|Trizzino, Marco; Barbieri, Elisa; Petracovici, Ana et al. (2018) The Tumor Suppressor ARID1A Controls Global Transcription via Pausing of RNA Polymerase II. Cell Rep 23:3933-3945|
|Shastrula, Prashanth Krishna; Lund, Peder J; Garcia, Benjamin A et al. (2018) Rpp29 regulates histone H3.3 chromatin assembly through transcriptional mechanisms. J Biol Chem 293:12360-12377|
|Kaur, Amanpreet; Ecker, Brett L; Douglass, Stephen M et al. (2018) Remodeling of the Collagen Matrix in Aging Skin Promotes Melanoma Metastasis and Affects Immune Cell Motility. Cancer Discov :|
Showing the most recent 10 out of 741 publications