The Biomolecular Resource Laboratory (BRL) is a heavily used core service laboratory of theComprehensive Cancer Center that supported the research laboratory activities of 41 Cancer Centermembers during fiscal year 2005. The BRL provides strategically important analytical services to Centermembers for 1) characterization of DMA, peptides, proteins and 2) identification and quantification of lipids,proteins, and peptides. These services include five different types of mass spectrometry for analysis ofproteins, peptides and lipids, automated DNA sequence analysis, synthesis of RNA and DNAoligonucleotides, purification of peptides and proteins, and chemical synthesis of peptides. The BRLanalyzed more than 14,000 samples during the reporting period. DNA sequence analysis (7924 samples)and mass spectrometry (6403 samples) were the most heavily used core services in the BRL. Theprotein/peptide and DNA chemical services accounted for an additional 956 samples. Laboratories of CancerCenter members received 49% of the services performed in the BRL during fiscal year 2005.The BRL has expanded significantly since the last review. Mass spectrometry services have been added tomeet the significant needs of Cancer Center members for metabolomics and proteomics. During the lastfunding period, three new mass spectrometers were added to the facility. These included two Bruker massspectrometers (an Autoflex MALDI-TOF and an Esquire HCT ion trap) and a Waters/Micromass QuadrupoleTime-of-flight (Q-TOF) mass spectrometer, specifically to enhance the study of proteins and lipids. Dr.Michael Thomas, a leading expert in the analysis of lipids and lipid metabolites by mass spectrometry, hasjoined the laboratory as a co-director with Dr. Mark Lively. The addition of Dr. Thomas, and his staff andinstrumentation, has enhanced the ability of the BRL to meet the needs of the Cancer Center programmembers in the especially important areas of proteomics and metabolomics. Together, Drs. Lively andThomas provide significant expertise in biochemistry of proteins and DNA, mass spectrometry, and analyticalmethods to enhance and promote cancer research in the CCCWFU.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Wake Forest University Health Sciences
United States
Zip Code
Chouliaras, Konstantinos; Levine, Edward A; Fino, Nora et al. (2017) Prognostic Factors and Significance of Gastrointestinal Leak After Cytoreductive Surgery (CRS) with Heated Intraperitoneal Chemotherapy (HIPEC). Ann Surg Oncol 24:890-897
Wang, Lulu; Habib, Amyn A; Mintz, Akiva et al. (2017) Phosphatidylserine-Targeted Nanotheranostics for Brain Tumor Imaging and Therapeutic Potential. Mol Imaging 16:1536012117708722
Keim-Malpass, Jessica; Mihalko, Shannon L; Russell, Greg et al. (2017) Problems Experienced by Ovarian Cancer Survivors During Treatment. J Obstet Gynecol Neonatal Nurs 46:544-554
Ivey, Jill W; Latouche, Eduardo L; Richards, Megan L et al. (2017) Enhancing Irreversible Electroporation by Manipulating Cellular Biophysics with a Molecular Adjuvant. Biophys J 113:472-480
Rohlfing, Matthew L; Mays, Ashley C; Isom, Scott et al. (2017) Insurance status as a predictor of mortality in patients undergoing head and neck cancer surgery. Laryngoscope 127:2784-2789
Soto-Pantoja, David R; Wilson, Adam S; Clear, Kenysha Yj et al. (2017) Unfolded protein response signaling impacts macrophage polarity to modulate breast cancer cell clearance and melanoma immune checkpoint therapy responsiveness. Oncotarget 8:80545-80559
Park, Sun H; Eber, Matthew R; Tsuzuki, Shunsuke et al. (2017) Adeno-associated virus serotype rh10 is a useful gene transfer vector for sensory nerves that innervate bone in immunodeficient mice. Sci Rep 7:17428
Andrews, Rachel N; Metheny-Barlow, Linda J; Peiffer, Ann M et al. (2017) Cerebrovascular Remodeling and Neuroinflammation is a Late Effect of Radiation-Induced Brain Injury in Non-Human Primates. Radiat Res 187:599-611
Carpenter, Richard L; Sirkisoon, Sherona; Zhu, Dongqin et al. (2017) Combined inhibition of AKT and HSF1 suppresses breast cancer stem cells and tumor growth. Oncotarget 8:73947-73963
Wajih, Nadeem; Basu, Swati; Jailwala, Anuj et al. (2017) Potential therapeutic action of nitrite in sickle cell disease. Redox Biol 12:1026-1039

Showing the most recent 10 out of 481 publications