Mission: The Bioanalytical Laboratory (BL) is a heavily used shared resource of the Comprehensive Cancer Center with a mission to provide access to advanced analytical services, technologies and scientific consultation for DNA, protein, and lipid chemistry. The BL specializes in discovery, identification, characterization, and quantification of biomolecules including: structural analysis of proteins, peptides and lipids by mass spectrometry and biochemical methods;DNA sequence analysis;DNA/RNA synthesis; chromatography of proteins, peptides, DNA, and lipids;and quantification of clinical biomarkers in tissue and blood specimens. Assets: Assets of the BL include more than 10 major instrument systems and the expertise of its staff members. Co-directors, Drs. Mark Lively and Michael Thomas, are expert chemists with more than 50 years of combined experience in research and core laboratory management. Dr. Thomas is a lipid mass spectrometry expert. Dr. Lively is a founding member and former president of the Association of Biomolecular Resource Facilities (ABRF). He is a member of the National Advisory Research Resources Council of the NIH National Center for Research Resources. The BL technicians have more than 80 years of combined laboratory experience. Usage: The BL supported the research of 73 Center members from 11/2009 -10/2010, analyzing 10,234 samples. The most heavily used services were: mass spectrometry methods (4,018 spls);DNA sequencing (4,299 spIs);biomarker HPLC (746 spIs);protein/peptide methods (248 spIs);and DNA synthesis (182 spIs). Center members received 60% of the services performed. Future directions: The BL recently added two important new mass spectrometers, a ThermoFisher TSQ Discovery Max electrospray, triple quadrupole mass spectrometer, to enhance the study of polar lipids and a ThermoFisher TSQ Quantum XLS triple quadrupole gas-chromatograph (GC) mass spectrometer. An Advion Nanomate? source was acquired for the Q-TOF mass spectrometer for proteomics. These instruments permit the BL to provide new and improved MS services of lipids, proteins, and peptides. Clinical biomarker analysis services will be expanded to enhance support of ongoing clinical studies.

Public Health Relevance

The BL is widely used by Center investigators from each program: 15 from Cell Growth and Survival;13 from Cellular Damage and Defense;and 7 from the Clinical Research Program. BL services provide specialized technologies to identify and characterize biological molecules to enable understanding their roles in basic biochemical mechanisms of cancer, cell proliferation, cell signaling, and DNA damage. The central availability of these shared resources insures availability, stability, reliability, and quality control.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
3P30CA012197-38S2
Application #
8714175
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
38
Fiscal Year
2013
Total Cost
$3,121
Indirect Cost
$1,081
Name
Wake Forest University Health Sciences
Department
Type
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157
Stuart, Christopher H; Singh, Ravi; Smith, Thomas L et al. (2016) Prostate-specific membrane antigen-targeted liposomes specifically deliver the Zn(2+) chelator TPEN inducing oxidative stress in prostate cancer cells. Nanomedicine (Lond) 11:1207-22
McIver, Zachariah A; Grayson, Jason M; Coe, Benjamin N et al. (2016) Targeting T Cell Bioenergetics by Modulating P-Glycoprotein Selectively Depletes Alloreactive T Cells To Prevent Graft-versus-Host Disease. J Immunol 197:1631-41
Paek, Min-So; Ip, Edward H; Levine, Beverly et al. (2016) Longitudinal Reciprocal Relationships Between Quality of Life and Coping Strategies Among Women with Breast Cancer. Ann Behav Med 50:775-783
Randle, Reese W; Swords, Douglas S; Levine, Edward A et al. (2016) Optimal extent of lymphadenectomy for gastric adenocarcinoma: A 7-institution study of the U.S. gastric cancer collaborative. J Surg Oncol 113:750-5
Pandya, Darpan N; Hantgan, Roy; Budzevich, Mikalai M et al. (2016) Preliminary Therapy Evaluation of (225)Ac-DOTA-c(RGDyK) Demonstrates that Cerenkov Radiation Derived from (225)Ac Daughter Decay Can Be Detected by Optical Imaging for In Vivo Tumor Visualization. Theranostics 6:698-709
Clark, Clancy J; Fino, Nora F; Clark, Norman et al. (2016) Trends in the Use of Endoscopic Retrograde Cholangiopancreatography for the Management of Chronic Pancreatitis in the United States. J Clin Gastroenterol 50:417-22
Navari, Rudolph M; Qin, Rui; Ruddy, Kathryn J et al. (2016) Olanzapine for the Prevention of Chemotherapy-Induced Nausea and Vomiting. N Engl J Med 375:134-42
Godwin, Ryan; Gmeiner, William; Salsbury Jr, Freddie R (2016) Importance of long-time simulations for rare event sampling in zinc finger proteins. J Biomol Struct Dyn 34:125-34
Ritchie, Melissa K; Johnson, Lynnette C; Clodfelter, Jill E et al. (2016) Crystal Structure and Substrate Specificity of Human Thioesterase 2: INSIGHTS INTO THE MOLECULAR BASIS FOR THE MODULATION OF FATTY ACID SYNTHASE. J Biol Chem 291:3520-30
Zahid, Osama K; Zhao, Boxuan Simen; He, Chuan et al. (2016) Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores. Sci Rep 6:29565

Showing the most recent 10 out of 407 publications