Mission: The objective of the CCCWFU Microarray Shared Resource is to provide Cancer Center members with a competitive and cutting-edge environment for cancer genomics research. The Shared Resource accomplishes this goal by providing faculty with comprehensive and cost-effective microarray technologies and bioinformatics support to facilitate RNA expression profiling, single nucleotide polymorphism (SNP) genotyping, genome copy-number analysis, and methylation profiling using Affymetrix GeneChip oligonucleotide arrays. The accomplishment of these objectives has, in part, been facilitated by recent modifications to the Shared Resource, including the recruitment of Dr. Lance D. Miller as Director, and the addition of a microarray bioinformatics expert to facilitate high quality, detailed analysis of microarray data. Assets: The Microarray Shared Resource has a modern infrastructure that includes the GeneChip Scanner 3000 7G multi-color scanner (recently upgraded to accommodate the MegAllele system for targeted genotyping);three GeneChip 450 fluidics stations;two GeneChip hybridization ovens and four high-speed computer workstations for data analysis workflows. Computational tools for data processing and low-level array analysis are provided by the Affymetrix GeneChip Command Console software (AGCC; released September 2008) which includes basic solutions for data normalization, analysis of expression, genotype and copy-number data, registration of samples and arrays, and management of multi-chip datasets. For advanced data analysis, the Shared Resource now maintains an annual license to the Partek Genomics Suite software - a comprehensive suite of advanced statistical methods and interactive data visualization tools. Usage &Future Directions: In the previous 1-year reporting period, the Microarray Shared Resource has been fully engaged, operating at near maximal capacity, performing 885 microarray hybridizations and providing technical and analytical resources to more than twice as many funded Center members as compared to previous years (1.8 to 5.5-fold more than seen in the past 3 years). Continued development of bioinformatics resources and acquisition of new array technologies are expected to further bolster cancer genomics research at the CCCWFU in the coming years.

Public Health Relevance

Cancer is a disease of the genome and microarray technologies are a valuable resource for investigating the genomic and molecular underpinnings of cancer formation, progression and response to treatment. Further development of the bioinformatics capabilities of the Shared Resource will be valuable to the clinical translation of discoveries made by Cancer Center investigators.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Wake Forest University Health Sciences
United States
Zip Code
Mirlohi, Susan; Duncan, Susan E; Harmon, Michele et al. (2015) Analysis of salivary fluid and chemosensory functions in patients treated for primary malignant brain tumors. Clin Oral Investig 19:127-37
Gmeiner, William H; Jennings-Gee, Jamie; Stuart, Christopher H et al. (2015) Thymineless death in F10-treated AML cells occurs via lipid raft depletion and Fas/FasL co-localization in the plasma membrane with activation of the extrinsic apoptotic pathway. Leuk Res 39:229-35
Sohl, Stephanie J; Levine, Beverly; Avis, Nancy E (2015) Evaluation of the Quality of Life in Adult Cancer Survivors (QLACS) scale for early post-treatment breast cancer survivors. Qual Life Res 24:205-12
Gmeiner, William H; Boyacioglu, Olcay; Stuart, Christopher H et al. (2015) The cytotoxic and pro-apoptotic activities of the novel fluoropyrimidine F10 towards prostate cancer cells are enhanced by Zn(2+) -chelation and inhibiting the serine protease Omi/HtrA2. Prostate 75:360-9
Randle, Reese W; Doud, Andrea N; Levine, Edward A et al. (2015) Peritoneal surface disease with synchronous hepatic involvement treated with Cytoreductive Surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC). Ann Surg Oncol 22:1634-8
Doud, Andrea N; Randle, Reese W; Clark, Clancy J et al. (2015) Impact of distal pancreatectomy on outcomes of peritoneal surface disease treated with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy. Ann Surg Oncol 22:1645-50
Yosipovitch, Gil; Mills, Kyle C; Nattkemper, Leigh A et al. (2014) Association of pain and itch with depth of invasion and inflammatory cell constitution in skin cancer: results of a large clinicopathologic study. JAMA Dermatol 150:1160-6
Milliron, Brandy-Joe; Vitolins, Mara Z; Tooze, Janet A (2014) Usual dietary intake among female breast cancer survivors is not significantly different from women with no cancer history: results of the National Health and Nutrition Examination Survey, 2003-2006. J Acad Nutr Diet 114:932-7
Jordan, Jennifer H; D'Agostino Jr, Ralph B; Hamilton, Craig A et al. (2014) Longitudinal assessment of concurrent changes in left ventricular ejection fraction and left ventricular myocardial tissue characteristics after administration of cardiotoxic chemotherapies using T1-weighted and T2-weighted cardiovascular magnetic resonan Circ Cardiovasc Imaging 7:872-9
Vatca, M; Lucas Jr, J T; Laudadio, J et al. (2014) Retrospective analysis of the impact of HPV status and smoking on mucositis in patients with oropharyngeal squamous cell carcinoma treated with concurrent chemotherapy and radiotherapy. Oral Oncol 50:869-76

Showing the most recent 10 out of 161 publications