Mission/Purpose: The Cellular Imaging Shared Resource provides access for Cancer Center members to advanced microscopy instruments, technical support for specimen preparation and scientific guidance in planning and applying these advanced microscopy techniques. Assets: The facility provides a full-service preparative laboratory for both light and electron microscopic techniques. Electron microscopy includes both transmission and scanning instruments, and the Shared Resource supports thin sectioning, surface sputter coating, critical point drying, negative staining and specimen embedding services. In addition, the Shared Resource supports routine microtomy and cryomicrotomy for both light and electron microscopy. Of the light microscopes housed in the Shared Resource, both inverted and upright formats are available for imaging with brightfield, phase contrast, Nomarski, dark field, and polarizing microscopy. Fluorescence microscopy using Zeiss and Olympus microscopes includes both widefield digital imaging in multiple spectra and confocal fluorescence imaging. Also, inverted microscopes equipped for time-lapse live cell experiments, single cell microinjection, and laser capture microdissection are available. A recent addition to Cellular Imaging is atomic force microscopy. This equipment has been purchased with assistance from the Comprehensive Cancer Center and further strengthens the scientific collaborations with Wake Forest physicists immensely. Usage: The Shared Resource gives priority to Cancer Center members for equipment access and operates a chargeback system that provides a 50% subsidy for membership. Cancer Center members represent approximately 50% or more of the Shared Resource's use. In the most recent year, 32 laboratories of Cancer Center members have utilized this Shared Resource. The access to advanced equipment is complemented by the expert guidance of Dr. Mark Willingham, Director of the Shared Resource. He, along with other technical experts on the Shared Resource support staff, assists investigators in the use of instruments and in planning of experiments using these instruments. Future Directions: This Shared Resource has recently added a new FEI transmission electron microscope to its instruments. In the near future, upgrades to a confocal system with "meta" capability and a UV laser are planned.

Public Health Relevance

Basic research in cancer often includes morphologic questions at the single cell level and at the level of complex tissues. Modern microscopy techniques provide critical tools to answer these questions. This shared resource houses microscopy equipment and expertise that provides Cancer Center members access to instruments that would ordinarily be beyond the capability of individual laboratories.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30CA012197-39
Application #
8617236
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
39
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Wake Forest University Health Sciences
Department
Type
DUNS #
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157
Stuart, Christopher H; Singh, Ravi; Smith, Thomas L et al. (2016) Prostate-specific membrane antigen-targeted liposomes specifically deliver the Zn(2+) chelator TPEN inducing oxidative stress in prostate cancer cells. Nanomedicine (Lond) 11:1207-22
McIver, Zachariah A; Grayson, Jason M; Coe, Benjamin N et al. (2016) Targeting T Cell Bioenergetics by Modulating P-Glycoprotein Selectively Depletes Alloreactive T Cells To Prevent Graft-versus-Host Disease. J Immunol 197:1631-41
Paek, Min-So; Ip, Edward H; Levine, Beverly et al. (2016) Longitudinal Reciprocal Relationships Between Quality of Life and Coping Strategies Among Women with Breast Cancer. Ann Behav Med 50:775-783
Randle, Reese W; Swords, Douglas S; Levine, Edward A et al. (2016) Optimal extent of lymphadenectomy for gastric adenocarcinoma: A 7-institution study of the U.S. gastric cancer collaborative. J Surg Oncol 113:750-5
Pandya, Darpan N; Hantgan, Roy; Budzevich, Mikalai M et al. (2016) Preliminary Therapy Evaluation of (225)Ac-DOTA-c(RGDyK) Demonstrates that Cerenkov Radiation Derived from (225)Ac Daughter Decay Can Be Detected by Optical Imaging for In Vivo Tumor Visualization. Theranostics 6:698-709
Clark, Clancy J; Fino, Nora F; Clark, Norman et al. (2016) Trends in the Use of Endoscopic Retrograde Cholangiopancreatography for the Management of Chronic Pancreatitis in the United States. J Clin Gastroenterol 50:417-22
Navari, Rudolph M; Qin, Rui; Ruddy, Kathryn J et al. (2016) Olanzapine for the Prevention of Chemotherapy-Induced Nausea and Vomiting. N Engl J Med 375:134-42
Godwin, Ryan; Gmeiner, William; Salsbury Jr, Freddie R (2016) Importance of long-time simulations for rare event sampling in zinc finger proteins. J Biomol Struct Dyn 34:125-34
Ritchie, Melissa K; Johnson, Lynnette C; Clodfelter, Jill E et al. (2016) Crystal Structure and Substrate Specificity of Human Thioesterase 2: INSIGHTS INTO THE MOLECULAR BASIS FOR THE MODULATION OF FATTY ACID SYNTHASE. J Biol Chem 291:3520-30
Zahid, Osama K; Zhao, Boxuan Simen; He, Chuan et al. (2016) Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores. Sci Rep 6:29565

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