Mission/Purpose: The Cellular Imaging Shared Resource provides access for Cancer Center members to advanced microscopy instruments, technical support for specimen preparation and scientific guidance in planning and applying these advanced microscopy techniques. Assets: The facility provides a full-service preparative laboratory for both light and electron microscopic techniques. Electron microscopy includes both transmission and scanning instruments, and the Shared Resource supports thin sectioning, surface sputter coating, critical point drying, negative staining and specimen embedding services. In addition, the Shared Resource supports routine microtomy and cryomicrotomy for both light and electron microscopy. Of the light microscopes housed in the Shared Resource, both inverted and upright formats are available for imaging with brightfield, phase contrast, Nomarski, dark field, and polarizing microscopy. Fluorescence microscopy using Zeiss and Olympus microscopes includes both widefield digital imaging in multiple spectra and confocal fluorescence imaging. Also, inverted microscopes equipped for time-lapse live cell experiments, single cell microinjection, and laser capture microdissection are available. A recent addition to Cellular Imaging is atomic force microscopy. This equipment has been purchased with assistance from the Comprehensive Cancer Center and further strengthens the scientific collaborations with Wake Forest physicists immensely. Usage: The Shared Resource gives priority to Cancer Center members for equipment access and operates a chargeback system that provides a 50% subsidy for membership. Cancer Center members represent approximately 50% or more of the Shared Resource's use. In the most recent year, 32 laboratories of Cancer Center members have utilized this Shared Resource. The access to advanced equipment is complemented by the expert guidance of Dr. Mark Willingham, Director of the Shared Resource. He, along with other technical experts on the Shared Resource support staff, assists investigators in the use of instruments and in planning of experiments using these instruments. Future Directions: This Shared Resource has recently added a new FEI transmission electron microscope to its instruments. In the near future, upgrades to a confocal system with """"""""meta"""""""" capability and a UV laser are planned.

Public Health Relevance

Basic research in cancer often includes morphologic questions at the single cell level and at the level of complex tissues. Modern microscopy techniques provide critical tools to answer these questions. This shared resource houses microscopy equipment and expertise that provides Cancer Center members access to instruments that would ordinarily be beyond the capability of individual laboratories.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA012197-39
Application #
8617236
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
2014-02-01
Budget End
2015-01-31
Support Year
39
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Wake Forest University Health Sciences
Department
Type
DUNS #
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157
Melvin, Ryan L; Xiao, Jiajie; Godwin, Ryan C et al. (2018) Visualizing correlated motion with HDBSCAN clustering. Protein Sci 27:62-75
Faig, Jennifer; Haughton, Michael; Taylor, Richard C et al. (2018) Retrospective Analysis of Cisplatin Nephrotoxicity in Patients With Head and Neck Cancer Receiving Outpatient Treatment With Concurrent High-dose Cisplatin and Radiotherapy. Am J Clin Oncol 41:432-440
Nelson, Kimberly J; Perkins, Arden; Van Swearingen, Amanda E D et al. (2018) Experimentally Dissecting the Origins of Peroxiredoxin Catalysis. Antioxid Redox Signal 28:521-536
Swanner, Jessica; Singh, Ravi (2018) Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models. Methods Mol Biol 1790:209-218
Holmila, Reetta J; Vance, Stephen A; Chen, Xiaofei et al. (2018) Mitochondria-targeted Probes for Imaging Protein Sulfenylation. Sci Rep 8:6635
Feliz-Mosquea, Yismeilin R; Christensen, Ashley A; Wilson, Adam S et al. (2018) Combination of anthracyclines and anti-CD47 therapy inhibit invasive breast cancer growth while preventing cardiac toxicity by regulation of autophagy. Breast Cancer Res Treat 172:69-82
Li, X C; Wang, M Y; Yang, M et al. (2018) A mutational signature associated with alcohol consumption and prognostically significantly mutated driver genes in esophageal squamous cell carcinoma. Ann Oncol 29:938-944
Rego, Stephen L; Harvey, Scott; Simpson, Sean R et al. (2018) TREX1 D18N mice fail to process erythroblast DNA resulting in inflammation and dysfunctional erythropoiesis. Autoimmunity :1-12
Lu, Yong; Wang, Qiang; Xue, Gang et al. (2018) Th9 Cells Represent a Unique Subset of CD4+ T Cells Endowed with the Ability to Eradicate Advanced Tumors. Cancer Cell 33:1048-1060.e7
Godwin, Ryan C; Macnamara, Lindsay M; Alexander, Rebecca W et al. (2018) Structure and Dynamics of tRNAMet Containing Core Substitutions. ACS Omega 3:10668-10678

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