The over-arching goal of the Mass Spectrometry/Proteomics (MSP) Shared Facility is to provide UA B Cancer Center members with state-of-the-art capabilities and training in mass spectrometry, proteomics, and bioanalytic technologies to support their research needs. The MSP accomplishes this by providing services to UAB Cancer Center members to identify, characterize, and quantify proteins, protein post -translational modifications, lipids, and small molecules pre sent in cells, biological fluids, and tissues. T he MSP Shared Facility is organized into four modules: 1) Bioanalytical Separation and Sample Preparation, 2) Proteomics, 3) Small Molecule Analytics, and 4) Data Analysis and Bioinformatics The modules are supported by Master's level and Ph.D. scientists who are experts in mass spectrometry, bioanalytical chemistry, statistics, systems biology, and information handling. Cancer Center members utilize mass spectrometry and proteomic approaches in their individual and collaborative studies t hat range from the identification of disease biomarkers to the development of tools for structural biology.
The specific aims of this CCC sponsored shared facility are to: 1) Provide high-quality and cost-effective mass spectrometry and proteomics services to Cancer Center members including the identification and quantification of proteins, protein modification s, small molecules, lipids, drugs, and metabolites in complex biological samples and statistical and systems biology analyses of mass spectrometry/proteomics datasets; 2) Develop new approaches and integrate advances in state-of-the-art technologies to support cancer research;and 3) Provide training and education to UAB Cancer Center members through seminars, participation in formal graduate courses, web-based information, and individual or team meetings. The MSP has used highly innovative methods to find biomarkers for early stage pancreatic cancer; biomarkers for use in studies of diet-cancer interaction s;and to identify changes in ser um proteins in response to environmental chemicals such as bis-phenols, and markers of poor-prognosis breast cancer.

Public Health Relevance

The ability to analyze proteins, lipids, and metabolites in complex biological samples provides critical insights into the action of chemotherapeutic drug;identification biological markers of disease diagnosis, progression, and therapeutic response;and changes in cellular pathways associated with malignant transformation. The MSP provides the specialized services critical to translating basic science discoveries to clinical applications in the treatment of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA013148-41
Application #
8732232
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-03-28
Project End
2016-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
41
Fiscal Year
2013
Total Cost
$194,757
Indirect Cost
$68,572
Name
University of Alabama Birmingham
Department
Type
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Nebane, N Miranda; Coric, Tatjana; McKellip, Sara et al. (2016) Acoustic Droplet Ejection Technology and Its Application in High-Throughput RNA Interference Screening. J Lab Autom 21:198-203
Zhang, Wei; Zhai, Ling; Wang, Yimin et al. (2016) Discovery of a novel inhibitor of kinesin-like protein KIFC1. Biochem J 473:1027-35
Carvajal, Felipe; Vallejos, Maricarmen; Walters, Beth et al. (2016) Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation. FEBS J 283:2508-27
Badiga, Suguna; Chambers, Michelle M; Huh, Warner et al. (2016) Expression of p16(INK4A) in cervical precancerous lesions is unlikely to be preventable by human papillomavirus vaccines. Cancer 122:3615-3623
Zhang, Wei; Zhai, Ling; Lu, Wenyan et al. (2016) Discovery of Novel Allosteric Eg5 Inhibitors Through Structure-Based Virtual Screening. Chem Biol Drug Des 88:178-87
Hull, Travis D; Boddu, Ravindra; Guo, Lingling et al. (2016) Heme oxygenase-1 regulates mitochondrial quality control in the heart. JCI Insight 1:e85817
Hegde, Shylaja; Kesterson, Robert A; Srivastava, Om P (2016) CRYβA3/A1-Crystallin Knockout Develops Nuclear Cataract and Causes Impaired Lysosomal Cargo Clearance and Calpain Activation. PLoS One 11:e0149027
Smith, M Ryan; Vayalil, Praveen K; Zhou, Fen et al. (2016) Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels. Redox Biol 8:136-48
McNally, Lacey R; Mezera, Megan; Morgan, Desiree E et al. (2016) Current and Emerging Clinical Applications of Multispectral Optoacoustic Tomography (MSOT) in Oncology. Clin Cancer Res 22:3432-9
Styles, Nathan A; Shonsey, Erin M; Falany, Josie L et al. (2016) Carboxy-terminal mutations of bile acid CoA:N-acyltransferase alter activity and substrate specificity. J Lipid Res 57:1133-43

Showing the most recent 10 out of 566 publications