The primary objective of the Molecular NMR Shared Facility is to provide NMR instrumentation and expertise for elucidating the structure and dynamic behavior of macromolecules. To achieve this goal, the facility provides training and educational activities for Cancer Center members in their research programs and drug discovery efforts. In addition, the facility provides expertise and technical support in experimental design and sample preparation. The Molecular NMR Shared Facility was one of the original shared facilities established in 1972 by the UAB Comprehensive Cancer Center, and continues to be a mainstay by providing critical, state-of-the-art resources. In just the last year, the NMR Facility has dramatically expanded its cutting-edge research capabilities in support of Cancer Center members. First, a grant to Dr. Rama Krishna from the NCRR High-End Instrumentation Program funded nearly $2 million for the acquisition of a state-of-the-art 800 MHz NMR System equipped with a cryoprobe. This 800 MHz NMR system will be devoted primarily for structural investigations on high-molecular weight systems. The installation of this 800 MHz NMR system is anticipated to be completed during the next few months, following the renovation of the proposed housing area in the Chemistry Building. Second, a state-of-the-art cryoprobe-equipped 700 MHz NMR system in the Chemistry Department has been added to the Cancer Center NMR Facility. This instrument will be devoted primarily for drug discovery and development research projects in the Cancer Center. Third, the existing 600 MHz NMR system was upgraded with a TCI cryoprobe. During the previous funding period, 98% of facility usage was devoted to support Cancer Center members from five different programs. CCC investigators who use the facility are involved in work on the design and synthesis of several novel anticancer drugs (paclitaxel conjugates, curcumin conjugates, nucleoside analogs, new retinoid derivatives active against breast and skin cancers, etc.), metastasis suppressor protein KISS1, protein interactions in cholangiocarcinoma, DNA oligonucleotides that induce TLR-9-mediated metastasis, structural studies on HIV1 proteins, etc. Continued NMR Facility support to these on-going projects as well as several new NCI-funded research projects is proposed during the next five-year period.

Public Health Relevance

NMR is a technology used in research to be able to understand three-dimensional structure at the atomic level in such a way that the dynamics of molecular motion and the interactions of drugs with targets can be accurately measured. This shared facility provides UAB CCC members with the capacity to analyze molecular interactions critical for both understanding basic mechanistic structures and using that information to refine potential therapeutics for cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA013148-41
Application #
8732233
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-03-28
Project End
2016-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
41
Fiscal Year
2013
Total Cost
$175,256
Indirect Cost
$68,572
Name
University of Alabama Birmingham
Department
Type
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Nebane, N Miranda; Coric, Tatjana; McKellip, Sara et al. (2016) Acoustic Droplet Ejection Technology and Its Application in High-Throughput RNA Interference Screening. J Lab Autom 21:198-203
Zhang, Wei; Zhai, Ling; Wang, Yimin et al. (2016) Discovery of a novel inhibitor of kinesin-like protein KIFC1. Biochem J 473:1027-35
Carvajal, Felipe; Vallejos, Maricarmen; Walters, Beth et al. (2016) Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation. FEBS J 283:2508-27
Badiga, Suguna; Chambers, Michelle M; Huh, Warner et al. (2016) Expression of p16(INK4A) in cervical precancerous lesions is unlikely to be preventable by human papillomavirus vaccines. Cancer 122:3615-3623
Zhang, Wei; Zhai, Ling; Lu, Wenyan et al. (2016) Discovery of Novel Allosteric Eg5 Inhibitors Through Structure-Based Virtual Screening. Chem Biol Drug Des 88:178-87
Hull, Travis D; Boddu, Ravindra; Guo, Lingling et al. (2016) Heme oxygenase-1 regulates mitochondrial quality control in the heart. JCI Insight 1:e85817
Hegde, Shylaja; Kesterson, Robert A; Srivastava, Om P (2016) CRYβA3/A1-Crystallin Knockout Develops Nuclear Cataract and Causes Impaired Lysosomal Cargo Clearance and Calpain Activation. PLoS One 11:e0149027
Smith, M Ryan; Vayalil, Praveen K; Zhou, Fen et al. (2016) Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels. Redox Biol 8:136-48
McNally, Lacey R; Mezera, Megan; Morgan, Desiree E et al. (2016) Current and Emerging Clinical Applications of Multispectral Optoacoustic Tomography (MSOT) in Oncology. Clin Cancer Res 22:3432-9
Styles, Nathan A; Shonsey, Erin M; Falany, Josie L et al. (2016) Carboxy-terminal mutations of bile acid CoA:N-acyltransferase alter activity and substrate specificity. J Lipid Res 57:1133-43

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