The goal of the Microarray Shared Facility is to provide comprehensive, state-of-the-art genomic analysis technologies to Cancer Center investigators. In accomplish this, the Microarray Shared Facility (MSF) provides whole genome and targeted gene expression analysis, high- and low-throughput whole genome and custom genotyping, whole genome methylation analysis, and targeted sequencing analysis to Cancer Center members and university investigators at large. The MSF was formerly the Gene Expression Shared Facility, and has both focused it's mission and extended access to core technologies related to the largescale analysis of gene expression. The facility has recently been expanded to include the iScan and BeadXpress systems from lllumina in order to complement the existing Affymetrix GeneChip system, which is comprised of the Affymetrix 3000 7G scanner with an autoloader, two FS450 fluidics stations and two Hyb 640 hybridization ovens. Because changes in technology can complicate comparison to previous studies, the MSF facility has the capacity to perform whole genome genotyping, copy number variation analysis, and gene expression microarrays on multiple systems in order to accommodate the needs of Cancer Center investigators who may have committed to working with one platform or another. In addition, we can perform microRNA profiling and whole genome methylation profiling using a Chromatin-IP protocol followed by hybridization on the human or mouse promoter chip (ChlP-Chip). The addition of the new platforms will provide Cancer Center members and university researchers several options when considering experimental design and data analysis. Together, our research team and laboratory resources will provide the necessary expertise and cutting-edge technology to support the proposed molecular studies described in this Comprehensive Cancer Center proposal.
The ability to analyze changes in gene and in gene expression rapidly is critical in order to understand the differences between cancer cells and normal tissue, and even among different cancer types that affect the same tissues (e.g. the many forms of breast or lung cancer). Genomic analysis is important in identifying genomic events associated with tumor initiation or progression, defining tumor types, and predicting response to therapies.
|Kim, H-G; LeGrand, J; Swindle, C S et al. (2017) The assembly competence domain is essential for inv(16)-associated acute myeloid leukemia. Leukemia 31:2267-2271|
|Miller, Aubrey L; Garcia, Patrick L; Pressey, Joseph G et al. (2017) Whole exome sequencing identified sixty-five coding mutations in four neuroblastoma tumors. Sci Rep 7:17787|
|Childers, Noel K; Grenett, Hernan; Morrow, Casey et al. (2017) Potential Risk for Localized Aggressive Periodontitis in African American Preadolescent Children. Pediatr Dent 39:294-298|
|Kasten, Benjamin B; Arend, Rebecca C; Katre, Ashwini A et al. (2017) B7-H3-targeted 212Pb radioimmunotherapy of ovarian cancer in preclinical models. Nucl Med Biol 47:23-30|
|Prince, Andrew C; Jani, Aditi; Korb, Melissa et al. (2017) Characterizing the detection threshold for optical imaging in surgical oncology. J Surg Oncol 116:898-906|
|McConnell, Matthew; Feng, Shengmei; Chen, Wei et al. (2017) Osteoclast proton pump regulator Atp6v1c1 enhances breast cancer growth by activating the mTORC1 pathway and bone metastasis by increasing V-ATPase activity. Oncotarget 8:47675-47690|
|Kumar, Ranjit; Yi, Nengjun; Zhi, Degui et al. (2017) Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile. NPJ Biofilms Microbiomes 3:12|
|Van Beusecum, J P; Zhang, S; Cook, A K et al. (2017) Acute toll-like receptor 4 activation impairs rat renal microvascular autoregulatory behaviour. Acta Physiol (Oxf) 221:204-220|
|Ghosh, Arindam P; Willey, Christopher D; Anderson, Joshua C et al. (2017) Kinomic profiling identifies focal adhesion kinase 1 as a therapeutic target in advanced clear cell renal cell carcinoma. Oncotarget 8:29220-29232|
|Shah, Spandan; Gibson, Andrew W; Ji, Chuanyi et al. (2017) Regulation of FcR? function by site-specific serine phosphorylation. J Leukoc Biol 101:421-428|
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