The Structural Biology Shared Resource provides state-of-the-art infrastructure and expertise needed for high quality protein production and high resolution structure determination by NMR and X-ray crystallography. These capabilities are complemented by significant strengths in cryo-electron microscopy, electron paramagnetic resonance, a wide range of vibrational spectroscopies, computational biology and unique macromolecular foot printing approaches at the College and AECC. Through the New York Structural Genomics Research Consortium (NYSGRC), AECC investigators have access to -25-30 shifts (8 hour blocks) on the X29 insertion device at the National Synchrotron Light Source (NSLS), Brookhaven National Laboratory, as well as outstanding access to the LRL-CAT beamline (administered by Eli Lilly &Co.) at the Advanced Photon Source (APS), which provides FEDEX crystallographic service at one of the premiere beamlines in the world. Moreover, through membership in the New York Structural Biology Center, AECC investigators have access to the X4A bending magnet beamline at NSLS. These arrangements ensure that access to data collection resources is never rate limiting. There is also in-house Rigaku rotating anode/image plate data collection capabilities essential for characterization and screening of samples prior to synchrotron analysis. NMR capabilities at Einstein consists of one 300 MHz and two 600 MHz NMR spectrometers, with a cryoprobe on one of the 600s. AECC investigators also have full access to the NYSBC making available two 900 MHz and three 800 MHz instruments, all with cryoprobes, and one 750 MHz solid state NMR spectrometer. As proposed in the previous application, the Structural Biology Shared Resource has established unique capabilities, including considerable automation and expertise, which support large-scale and high-throughput protein production in E. coli, insect cell and mammalian expression platforms. Additional automation supports high-throughput protein crystallization and crystal imaging. The Facility is now working to implement modest fragment-based screening capabilities to leverage the numerous proteins and structures being produced, and is positioned to exploit NSLS-II, the new third generation synchrotron facility being constructed at Brookhaven National Laboratory. Together, these capabilities provide the AECC community with a powerful infrastructure that will make it possible to realize protein reagents and structural information to drive a wide range of biology and therapeutics.

Public Health Relevance

The Structural Biology Shared Resource provides unparalleled expertise and instrumentation for the production of protein reagents and high resolution structure determination to support the translational research mission and goals of the Albert Einstein Cancer Center (AECC). As an NCI-designated Cancer Center, AECC contributes to the national effort to reduce morbidity and mortality from cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA013330-40
Application #
8582100
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-06-01
Project End
2018-06-30
Budget Start
2013-08-23
Budget End
2014-06-30
Support Year
40
Fiscal Year
2013
Total Cost
$136,784
Indirect Cost
$51,765
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Peregrina, Karina; Houston, Michele; Daroqui, Cecilia et al. (2015) Vitamin D is a determinant of mouse intestinal Lgr5 stem cell functions. Carcinogenesis 36:25-31
Kim, Ryung S (2015) A new comparison of nested case-control and case-cohort designs and methods. Eur J Epidemiol 30:197-207
Arora, Pooja; Baena, Andres; Yu, Karl O A et al. (2014) A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens. Immunity 40:105-16
Stanley, Pamela (2014) Galectin-1 Pulls the Strings on VEGFR2. Cell 156:625-6
Yamane, Fumihiro; Nishikawa, Yumiko; Matsui, Kazue et al. (2014) CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34. J Leukoc Biol 95:19-31
Singh, M; Quispe-Tintaya, W; Chandra, D et al. (2014) Direct incorporation of the NKT-cell activator ?-galactosylceramide into a recombinant Listeria monocytogenes improves breast cancer vaccine efficacy. Br J Cancer 111:1945-54
Bahde, Ralf; Kapoor, Sorabh; Viswanathan, Preeti et al. (2014) Endothelin-1 receptor A blocker darusentan decreases hepatic changes and improves liver repopulation after cell transplantation in rats. Hepatology 59:1107-17
Ho, Gloria Y F; Wang, Tao; Zheng, Siqun L et al. (2014) Circulating soluble cytokine receptors and colorectal cancer risk. Cancer Epidemiol Biomarkers Prev 23:179-88
Ghartey, Jeny P; Smith, Benjamin C; Chen, Zigui et al. (2014) Lactobacillus crispatus dominant vaginal microbiome is associated with inhibitory activity of female genital tract secretions against Escherichia coli. PLoS One 9:e96659
Kerns, Sarah L; de Ruysscher, Dirk; Andreassen, Christian N et al. (2014) STROGAR - STrengthening the Reporting Of Genetic Association studies in Radiogenomics. Radiother Oncol 110:182-8

Showing the most recent 10 out of 938 publications