Imaging plays a vital role in the research programs of many AECC members. The Analytical Imaging Facility (AIF) makes routine and complex imaging technologies available to the entire AECC community. Microscope technology ranges from traditional brightfield, to epi-fluorescence, to laser scanning confocal, to high resolution transmission and scanning electron microscopy including cryoEM of macromolecules in this truly comprehensive shared resource. This wide range of imaging modalities allows for the study of animal models of disease. Cells expressing or labeled with fluorescent reporter molecules may be imaged in the whole animal, whole organ, single cell or cell compartments. Live cells may be imaged in real time to monitor physiological processes. Photo-activatable fluorescence molecules may be manipulated by light for dynamic in vivo studies. Confocal microscopes and epi-fluorescence paired with deconvolution enables 3D imaging of fixed material or 4D imaging of live cells. Traditional ultrastructure analysis by transmission electron microscopy is expanded to 3D by electron tomography. The recent installation of a field emission scanning electron microscope (SEM) has expanded the facility's SEM capabilities to include automated large area mapping, 3D image reconstruction, x-ray microanalysis, correlative fluorescence and SEM imaging and imaging frozen hydrated samples. AIF staff assist users in experimental design, data collection, quantitative image analysis and presentation. New users are taught imaging techniques required for their specific research objective, while experienced users may customize any imaging station, utilizing the facility's large inventory of optics and accessories. The facility offers customized full service sample preparation for electron microscopy, to ensure quality control and to offer the widest range of techniques. The AIF staff has the expertise to prepare samples by methods that include chemical fixation, embedding in resin, ultrathin sectioning, immunogold labeling and negative staining. The facility offers a full range of low temperature techniques for electron microscopy including quick freezing by plunge, metal mirror or high pressure freezing. Frozen samples can undergo freeze-substitution, freeze fracture, rotary shadow or cryosectioning and are then imaged in the TEM or SEM at ambient or low temperature. The AIF provides support for the microscopy requirements of the Molecular Cytogenetics and Histopathology Shared Resources. Since the last CCSG review, important new additions of equipment, personnel and services have been made, which have substantially enhanced the imaging capabilities at AECC.
The Analytical Imaging Facility provides microscope imaging technologies and quantitative image analysis supporting the translational research mission and goals of the Albert Einstein Cancer Center (AECC). As an NCI-designated Cancer Center, AECC contributes to the national effort to reduce morbidity and mortality from cancer.
|Peregrina, Karina; Houston, Michele; Daroqui, Cecilia et al. (2015) Vitamin D is a determinant of mouse intestinal Lgr5 stem cell functions. Carcinogenesis 36:25-31|
|Kim, Ryung S (2015) A new comparison of nested case-control and case-cohort designs and methods. Eur J Epidemiol 30:197-207|
|Arora, Pooja; Baena, Andres; Yu, Karl O A et al. (2014) A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens. Immunity 40:105-16|
|Stanley, Pamela (2014) Galectin-1 Pulls the Strings on VEGFR2. Cell 156:625-6|
|Yamane, Fumihiro; Nishikawa, Yumiko; Matsui, Kazue et al. (2014) CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34. J Leukoc Biol 95:19-31|
|Singh, M; Quispe-Tintaya, W; Chandra, D et al. (2014) Direct incorporation of the NKT-cell activator ?-galactosylceramide into a recombinant Listeria monocytogenes improves breast cancer vaccine efficacy. Br J Cancer 111:1945-54|
|Bahde, Ralf; Kapoor, Sorabh; Viswanathan, Preeti et al. (2014) Endothelin-1 receptor A blocker darusentan decreases hepatic changes and improves liver repopulation after cell transplantation in rats. Hepatology 59:1107-17|
|Ho, Gloria Y F; Wang, Tao; Zheng, Siqun L et al. (2014) Circulating soluble cytokine receptors and colorectal cancer risk. Cancer Epidemiol Biomarkers Prev 23:179-88|
|Ghartey, Jeny P; Smith, Benjamin C; Chen, Zigui et al. (2014) Lactobacillus crispatus dominant vaginal microbiome is associated with inhibitory activity of female genital tract secretions against Escherichia coli. PLoS One 9:e96659|
|Kerns, Sarah L; de Ruysscher, Dirk; Andreassen, Christian N et al. (2014) STROGAR - STrengthening the Reporting Of Genetic Association studies in Radiogenomics. Radiother Oncol 110:182-8|
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