The Flow Cytometry Shared Resource (FACS-SR) provides AECC investigators with technologies required for fluorescence-based cell analysis and sorting. A broad range of research in a large proportion of AECC associated laboratories is supported by the FACS-SR, with connections to virtually all AECC programs and training activities. The instruments required for flow cytometry and cell sorting are intricate and expensive, making this a logical resource to provide through a centralized facility. In addition, cell sorting presents complex issues in biocontainment, requiring specialized laboratory space and highly trained personnel for proper management. The organization of these resources within the FACS-SR has proven to be an extremely efficient approach to creating a well supervised, quality controlled, cost effective and safe environment for FACS-based analysis and cell sorting. Since the last CCSG review in 2007, the FACS-SR has undergone major expansion to add new capabilities as well as a needed increase in the capacity for all services. Substantial additional laboratory space has been added, including rooms that are specially configured for enhanced BSL-2 work involved with sorting of primary human cells or potentially infectious material. Work performed to a great extent by AECC members has driven technology development in the FACS-SR, and supported multiple successful NIH Shared Instrumentation Grants over the past five years. This, along with substantial ongoing institutional support, has led to the acquisition of major new equipment and expansion of the resources and services provided by the FACS-SR. Three new cell sorters have been added to the facility since the last competing renewal, enabling accommodation of the growing need for this resource. Both Beckman-Coulter MoFlo and BD FACSAria sorters are operated by the facility, providing an extraordinarily advanced and versatile range of cell sorting applications. In addition, several analytical flow cytometers have been added to the facility's inventory, and facility staff has been increased to achieve maximum utilization of the instrumentation. The FACS-SR has also been highly successful at introducing novel technologies to AECC investigators in recent years, such as laser scanning cytometry and newly derived fluorescent proteins with unique spectral properties. The FACS-SR staff provides training on all the instruments, as well as consultation to assist with experimental design and instrument selection. A well established administrative structure ensures orderly management of facility operations and efficient delivery of services at the lowest possible cost.
The Flow Cytometry facility provides a highly efficient, cost effective and safe environment for flow cytometry-based analysis and cell sorting experiments supporting the translational research mission and goals of the Albert Einstein Cancer Center (AECC). As an NCI-designated Cancer Center, AECC contributes to the national effort to reduce morbidity and mortality from cancer.
|Mao, Serena P H; Park, Minji; Cabrera, Ramon M et al. (2018) Loss of amphiregulin reduces myoepithelial cell coverage of mammary ducts and alters breast tumor growth. Breast Cancer Res 20:131|
|Mocholi, Enric; Dowling, Samuel D; Botbol, Yair et al. (2018) Autophagy Is a Tolerance-Avoidance Mechanism that Modulates TCR-Mediated Signaling and Cell Metabolism to Prevent Induction of T Cell Anergy. Cell Rep 24:1136-1150|
|Yang, Chia-Ping Huang; Wang, Changwei; Ojima, Iwao et al. (2018) Taxol Analogues Exhibit Differential Effects on Photoaffinity Labeling of ?-Tubulin and the Multidrug Resistance Associated P-Glycoprotein. J Nat Prod 81:600-606|
|Guan, Fangxia; Tabrizian, Tahmineh; Novaj, Ardijana et al. (2018) Dietary Walnuts Protect Against Obesity-Driven Intestinal Stem Cell Decline and Tumorigenesis. Front Nutr 5:37|
|Wang, Tao; Hosgood, H Dean; Lan, Qing et al. (2018) The Relationship Between Population Attributable Fraction and Heritability in Genetic Studies. Front Genet 9:352|
|Chennamadhavuni, Divya; Saavedra-Avila, Noemi Alejandra; Carreño, Leandro J et al. (2018) Dual Modifications of ?-Galactosylceramide Synergize to Promote Activation of Human Invariant Natural Killer T Cells and Stimulate Anti-tumor Immunity. Cell Chem Biol 25:571-584.e8|
|Limi, Saima; Senecal, Adrien; Coleman, Robert et al. (2018) Transcriptional burst fraction and size dynamics during lens fiber cell differentiation and detailed insights into the denucleation process. J Biol Chem 293:13176-13190|
|Rocha, Agostinho G; Franco, Antonietta; Krezel, Andrzej M et al. (2018) MFN2 agonists reverse mitochondrial defects in preclinical models of Charcot-Marie-Tooth disease type 2A. Science 360:336-341|
|Cabahug-Zuckerman, Pamela; Stout Jr, Randy F; Majeska, Robert J et al. (2018) Potential role for a specialized ?3 integrin-based structure on osteocyte processes in bone mechanosensation. J Orthop Res 36:642-652|
|Vilchèze, Catherine; Copeland, Jacqueline; Keiser, Tracy L et al. (2018) Rational Design of Biosafety Level 2-Approved, Multidrug-Resistant Strains of Mycobacterium tuberculosis through Nutrient Auxotrophy. MBio 9:|
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