The Genomics Shared Resource provides a broad spectrum of the latest genomic and epigenomic technologies to AECC members. The facility has undergone major changes since the last CCSG review. The facility has moved to a new location in a new building. New equipment has been acquired and support personnel have been added, resulting in the implementation of new technologies and services for AECC members. Massively parallel sequencing has been implemented providing whole exome sequencing, whole transcriptome sequencing, and mlRNA-Seq. Massively parallel sequencing also supports epigenetic studies including genome-wide assessment of cytosine methylation based on the HELP assay (Hpall tiny fragment Enrichment by Ligation-mediated PCR assay), Chlp-Seq, and MethylCSeq. The facility also provides Pyrosequencing and Sequenom analyses, Fluidigm assays and Affymetrix arrays. Some of the. equipment recently acquired to support these new services include: Sequenom MALDI-ToF System, Caliper GX and XT (automated microcapillary electrophoresis);Fluidigm Biomark and Access Array System;lllumina Genome Analyzer II massively parallel sequencer;two lllumina HiSeq 2000 massively parallel sequencers, and Tecan Evo 200 Liquid Handling Automation Instrument. The lllumina MiSeq instrument was recently purchased for medium throughput massively parallel sequencing and the lon Torrent Proton instrument for quick whole genome sequencing will be installed shortly. Labor intensive assays are being converted to more efficient robot based methods.

Public Health Relevance

The Genomjcs Shared Resource provides both genomic and epigenomic assays (DNA, RNA, methylation, ChlPseq, miRNA-seq) in an efficient and cost-effective core facility supporting the research mission and goals of the Albert Einstein Cancer Center (AECC). As an NCI-designated Cancer Center, AECC contributes to the national effort to reduce the morbidity and mortality from cancer.

National Institute of Health (NIH)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Albert Einstein College of Medicine
United States
Zip Code
Peregrina, Karina; Houston, Michele; Daroqui, Cecilia et al. (2015) Vitamin D is a determinant of mouse intestinal Lgr5 stem cell functions. Carcinogenesis 36:25-31
Kim, Ryung S (2015) A new comparison of nested case-control and case-cohort designs and methods. Eur J Epidemiol 30:197-207
Arora, Pooja; Baena, Andres; Yu, Karl O A et al. (2014) A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens. Immunity 40:105-16
Stanley, Pamela (2014) Galectin-1 Pulls the Strings on VEGFR2. Cell 156:625-6
Yamane, Fumihiro; Nishikawa, Yumiko; Matsui, Kazue et al. (2014) CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34. J Leukoc Biol 95:19-31
Singh, M; Quispe-Tintaya, W; Chandra, D et al. (2014) Direct incorporation of the NKT-cell activator ?-galactosylceramide into a recombinant Listeria monocytogenes improves breast cancer vaccine efficacy. Br J Cancer 111:1945-54
Bahde, Ralf; Kapoor, Sorabh; Viswanathan, Preeti et al. (2014) Endothelin-1 receptor A blocker darusentan decreases hepatic changes and improves liver repopulation after cell transplantation in rats. Hepatology 59:1107-17
Ho, Gloria Y F; Wang, Tao; Zheng, Siqun L et al. (2014) Circulating soluble cytokine receptors and colorectal cancer risk. Cancer Epidemiol Biomarkers Prev 23:179-88
Ghartey, Jeny P; Smith, Benjamin C; Chen, Zigui et al. (2014) Lactobacillus crispatus dominant vaginal microbiome is associated with inhibitory activity of female genital tract secretions against Escherichia coli. PLoS One 9:e96659
Kerns, Sarah L; de Ruysscher, Dirk; Andreassen, Christian N et al. (2014) STROGAR - STrengthening the Reporting Of Genetic Association studies in Radiogenomics. Radiother Oncol 110:182-8

Showing the most recent 10 out of 938 publications