A Cancer Center-managed facility, the Molecular Pathology Shared Resource continues to provide access to well characterized human normal and tumor tissues, macromolecule (DNA/RNA) extraction/quantification, and expert histopathology evaluation. This Shared Resource remains highly in demand with heavy utilization by members of the Herbert Irving Comprehensive Cancer Center (HICCC). Services provided include: ? Tissue Procurement, banking and distribution to investigators ? DNA/RNA extraction, banking and distribution to investigators for molecular biology studies ? Histology, tissue microarray construction and immunohistochemistry for investigator-initiated projects The tumor banking service includes the procurement, storage and distribution of normal and tumor samples from Columbia University Medical Center (CUMC). The bank follows CUMC IRB guidelines, and has multiple security layers to ensure sample safety. The macromolecular service provides DNA/RNA isolation and quantitation, assisting with ancillary techniques, such as tissue microdissection. The histology service provides a full array of tissue-based methods, including tissue grossing, processing, frozen section cutting, standardized and specialized histochemical staining. It also offers expert systematic histopathologic evaluation of human and experimental tissue samples, and assists in routine tissue microarray construction. In addition, it offers immunohistochemistry (IHC) and in situ hybridization (ISH) in order to define expression patterns. Future plans center on further developing and implementing novel techniques in image analysis and quantitative biomarker multiplexing at the microanatomical detail ("systems pathology"). The facility is also generating tissue pharmacodynamic studies to further define biological endpoints and valuable biomarkers to assist in clinical trials. Finally, working in close association with the Biomedical Informatics Resource, integration of biospecimen management, clinical and molecular data is pursued following caBIG guidelines, and using novel tools, such as caTissue (biospecimen management) and calntegrator (integration of clinicopathological and genomic data). During the last period of the CCSG* 30% of the investigators using the facility are Cancer Center members with peer-reviewed funding, with those members representing from 30% to 50% of the usage of the established services. The proposed total operating budget of the facility is $843,715, of which we are requesting $ 255,887 from the CCSG.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Columbia University (N.Y.)
New York
United States
Zip Code
Bassuk, Alexander G; Sujirakul, Tharikarn; Tsang, Stephen H et al. (2014) A novel RPGR mutation masquerading as Stargardt disease. Br J Ophthalmol 98:709-11
Li, Yao; Wu, Wen-Hsuan; Hsu, Chun-Wei et al. (2014) Gene therapy in patient-specific stem cell lines and a preclinical model of retinitis pigmentosa with membrane frizzled-related protein defects. Mol Ther 22:1688-97
Wert, Katherine J; Sancho-Pelluz, Javier; Tsang, Stephen H (2014) Mid-stage intervention achieves similar efficacy as conventional early-stage treatment using gene therapy in a pre-clinical model of retinitis pigmentosa. Hum Mol Genet 23:514-23
Shen, Sherry; Sujirakul, Tharikarn; Tsang, Stephen H (2014) Next-generation sequencing revealed a novel mutation in the gene encoding the beta subunit of rod phosphodiesterase. Ophthalmic Genet 35:142-50
Palomero, Teresa; Couronné, Lucile; Khiabanian, Hossein et al. (2014) Recurrent mutations in epigenetic regulators, RHOA and FYN kinase in peripheral T cell lymphomas. Nat Genet 46:166-70
Higuchi-Sanabria, Ryo; Pernice, Wolfgang M A; Vevea, Jason D et al. (2014) Role of asymmetric cell division in lifespan control in Saccharomyces cerevisiae. FEMS Yeast Res 14:1133-46
Lam, A T; Curschellas, C; Krovvidi, D et al. (2014) Controlling self-assembly of microtubule spools via kinesin motor density. Soft Matter 10:8731-6
Olszak, Torsten; Neves, Joana F; Dowds, C Marie et al. (2014) Protective mucosal immunity mediated by epithelial CD1d and IL-10. Nature 509:497-502
Murtomaki, Aino; Uh, Minji K; Kitajewski, Chris et al. (2014) Notch signaling functions in lymphatic valve formation. Development 141:2446-51
Nong, Eva; Lee, Winston; Merriam, Joanna E et al. (2014) Disease progression in autosomal dominant cone-rod dystrophy caused by a novel mutation (D100G) in the GUCA1A gene. Doc Ophthalmol 128:59-67

Showing the most recent 10 out of 142 publications