Abstract

The Biopolymers Division provides routine Sanger DNA sequencing in addition to next-generation genomic DNA sequencing (Illumine), peptide synthesis and purification, peptide arrays, basic mass spectrometry, high-pressure liquid chromatographic analysis and purification, and other services. The Proteomics Division provides services related to the identification, characterization, and quantitation of proteins from simple to complex mixtures. Approaches include the use of 1 D-gel, 2D-gel, or chromatographic fractionation methods followed by mass spectrometric analysis. Stable isotope derivatization and specialized software are available for quantitation of differential protein expression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014051-43
Application #
8680157
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
43
Fiscal Year
2014
Total Cost
$197,910
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Guen, Vincent J; Edvardson, Simon; Fraenkel, Nitay D et al. (2018) A homozygous deleterious CDK10 mutation in a patient with agenesis of corpus callosum, retinopathy, and deafness. Am J Med Genet A 176:92-98
Murphy, Patrick A; Butty, Vincent L; Boutz, Paul L et al. (2018) Alternative RNA splicing in the endothelium mediated in part by Rbfox2 regulates the arterial response to low flow. Elife 7:
Khan, Omar F; Kowalski, Piotr S; Doloff, Joshua C et al. (2018) Endothelial siRNA delivery in nonhuman primates using ionizable low-molecular weight polymeric nanoparticles. Sci Adv 4:eaar8409
Woolston, Benjamin M; Roth, Timothy; Kohale, Ishwar et al. (2018) Development of a formaldehyde biosensor with application to synthetic methylotrophy. Biotechnol Bioeng 115:206-215
Huang, Hsin-Ho; Qian, Yili; Del Vecchio, Domitilla (2018) A quasi-integral controller for adaptation of genetic modules to variable ribosome demand. Nat Commun 9:5415
Guimarães, Pedro P G; Gaglione, Stephanie; Sewastianik, Tomasz et al. (2018) Nanoparticles for Immune Cytokine TRAIL-Based Cancer Therapy. ACS Nano 12:912-931
Moynihan, Kelly D; Holden, Rebecca L; Mehta, Naveen K et al. (2018) Enhancement of Peptide Vaccine Immunogenicity by Increasing Lymphatic Drainage and Boosting Serum Stability. Cancer Immunol Res 6:1025-1038
Nath, Samir R; Yu, Zhigang; Gipson, Theresa A et al. (2018) Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction. J Clin Invest 128:3630-3641
Kimmerling, Robert J; Prakadan, Sanjay M; Gupta, Alejandro J et al. (2018) Linking single-cell measurements of mass, growth rate, and gene expression. Genome Biol 19:207
Rothenberg, Daniel A; Taliaferro, J Matthew; Huber, Sabrina M et al. (2018) A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth. iScience 9:367-381

Showing the most recent 10 out of 904 publications