The Transgenic and Knockout Mouse Facility provides services for the production of genetically altered mice. The Facility maintains expertise in microinjection of foreign DNA, including large BACs (bacterial artifical chromosomes) into fertilized mouse embryos, as well as skills needed for successful gene targeting experiments using pluripotent mouse embryonic stem cells (ES cells.) The Facility has routinely produced mice from ES cells carrying mutations developed elsewhere in high throughput screens, from resources such as KOMP, Baygenomics, Sanger Institute Genetrap Resource and others. A successful rederivation service has also been provided by thawing embryos cryopreserved in straws or tubes and also """"""""cleaning up"""""""" mice from live stocks carrying pathogens using embryo washing techniques and oviduct transfer. Numerous gene """"""""knockouts,"""""""" single gene delefions, large deletions, point mutafions, condifional mutafions, gene exchanges (""""""""knock-ins"""""""") and other sophisticated mutations have been generated by the facility. Engineered mutations allow investigators to study gene expression and function in the context of the whole organism and during dynamic processes such as embryonic and post natal development, tissue regeneration and repair, cancer induction and progression, response to environmental factors and aging. In addition, the modifying effect of mutations and genetic background on a disease process can be investigated. In the study of diseases as complex as cancer, genetically engineered mice are essential tools which can provide important and novel insights. The scientific literature is filled with examples of mice that serve as models of human disease. A Comprehensive Cancer Institute is best served by a responsive, on-site Transgenic Facility that can become a loyal partner in projects initiated by Cancer Institute members who seek to understand the complexities of the disease of cancer. The Shared Facility contributes expertise, equipment and other resources to effectively move individual projects fon^/ard in a timely manner. Key personnel in the Transgenic Facility have a long history of dedicated service to the Duke Comprehensive Cancer Institute. The shared resource manager, Cheryl Bock, has held this position since the start of the facility 20 years ago. Research Technician Meilang Flowers, has provided diligent support for 9 years. Quality service from experienced people is a significant reason for the excellent success of this Shared Resource.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014236-40
Application #
8601834
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
40
Fiscal Year
2014
Total Cost
$144,372
Indirect Cost
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Galaz-Montoya, Monica; Wright, Sara J; Rodriguez, Gustavo J et al. (2017) ?2-Adrenergic receptor activation mobilizes intracellular calcium via a non-canonical cAMP-independent signaling pathway. J Biol Chem 292:9967-9974
Lanier, Megan L; Park, Hyeri; Mukherjee, Paramita et al. (2017) Formal Synthesis of (+)-Laurencin by Gold(I)-Catalyzed Intramolecular Dehydrative Alkoxylation. Chemistry 23:7180-7184
Shi, Qiong; Liu, Hongliang; Han, Peng et al. (2017) Genetic Variants in WNT2B and BTRC Predict Melanoma Survival. J Invest Dermatol 137:1749-1756
Woyach, Jennifer A; Ruppert, Amy S; Guinn, Daphne et al. (2017) BTKC481S-Mediated Resistance to Ibrutinib in Chronic Lymphocytic Leukemia. J Clin Oncol 35:1437-1443
Wang, Yanru; Freedman, Jennifer A; Liu, Hongliang et al. (2017) Associations between RNA splicing regulatory variants of stemness-related genes and racial disparities in susceptibility to prostate cancer. Int J Cancer 141:731-743
Fayanju, Oluwadamilola M; Hall, Carolyn S; Bauldry, Jessica Bowman et al. (2017) Body mass index mediates the prognostic significance of circulating tumor cells in inflammatory breast cancer. Am J Surg 214:666-671
Leu, David; Spasojevic, Ivan; Nguyen, Huy et al. (2017) CNS bioavailability and radiation protection of normal hippocampal neurogenesis by a lipophilic Mn porphyrin-based superoxide dismutase mimic, MnTnBuOE-2-PyP5. Redox Biol 12:864-871
Sauer, Scott J; Tarpley, Michael; Shah, Imran et al. (2017) Bisphenol A activates EGFR and ERK promoting proliferation, tumor spheroid formation and resistance to EGFR pathway inhibition in estrogen receptor-negative inflammatory breast cancer cells. Carcinogenesis 38:252-260
Pan, Yongchu; Liu, Hongliang; Wang, Yanru et al. (2017) Associations between genetic variants in mRNA splicing-related genes and risk of lung cancer: a pathway-based analysis from published GWASs. Sci Rep 7:44634
Yin, Jieyun; Liu, Hongliang; Liu, Zhensheng et al. (2017) Pathway-analysis of published genome-wide association studies of lung cancer: A potential role for the CYP4F3 locus. Mol Carcinog 56:1663-1672

Showing the most recent 10 out of 480 publications