The DNA Microarray Facility provides access to various forms of technology for gene expression analysis, pairticularly DNA microarray technologies, to members ofthe Duke Comprehensive Caricer Institute. This includes Affymetrix microarrays as well as printed DNA microarrays produced in the facility. Importantly, the facility also provides service for microarray analysis, either with Affymetrix arrays or printed oligonucleotide arrays. As a result of the volume of use, the facility has negotiated an agreement with Affymetrix that now provides a very substantial discount in cost of microarrays and services, to the benefit of Cancer Institute members. Beyond the microarray services, the facility also provides Cancer Institute members access to an ABI 7900 RT-PCR instrument for validafion of gene expression results as well as alternative methods of expression assay, and access to an Axon Genepix Scanner for those wanting to scan their own arrays. In addifion, the facility provides substantial support for data analysis and management of microarray datasets. These services include inifial consultafion in experimental design, assessment of RNA quality, generafion of probes, hybridization to arrays, scanning of arrays, data analysis, and data management and storage. The Microarray Core Facility developed and uses a custom LIMS that integrates every point ofthe data production process, starting with sample submissions from researchers to laboratory operations, and then to final billing and posfings. With the LIMS tools and the repository features ofthe Express data repository, data of different types can be grouped and managed by projects, and because ofthe simplicity ofthe web-based interfaces that have been developed, researchers can execute their own analysis and data management in a simplified fashion. The impact of the facility on research in the Cancer Institute has been substantial, as evidenced by the large number of publications as well as the translation of these services into clinical practice in the form of multiple investigator-initiated clinical trials making use of DNA microarray analysis to guide clinical decisions.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014236-40
Application #
8601837
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
40
Fiscal Year
2014
Total Cost
$166,923
Indirect Cost
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
McDonnell, Eoin; Crown, Scott B; Fox, Douglas B et al. (2016) Lipids Reprogram Metabolism to Become a Major Carbon Source for Histone Acetylation. Cell Rep 17:1463-1472
Liu, Hongliang; Gao, Fengqin; Dahlstrom, Kristina R et al. (2016) A variant at a potentially functional microRNA-binding site in BRIP1 was associated with risk of squamous cell carcinoma of the head and neck. Tumour Biol 37:8057-66
Evans, M K; Sauer, S J; Nath, S et al. (2016) X-linked inhibitor of apoptosis protein mediates tumor cell resistance to antibody-dependent cellular cytotoxicity. Cell Death Dis 7:e2073
Pollock, Julie A; Wardell, Suzanne E; Parent, Alexander A et al. (2016) Inhibiting androgen receptor nuclear entry in castration-resistant prostate cancer. Nat Chem Biol 12:795-801
He, Kevin; Li, Yanming; Zhu, Ji et al. (2016) Component-wise gradient boosting and false discovery control in survival analysis with high-dimensional covariates. Bioinformatics 32:50-7
Yuan, Hua; Liu, Hongliang; Liu, Zhensheng et al. (2016) A Novel Genetic Variant in Long Non-coding RNA Gene NEXN-AS1 is Associated with Risk of Lung Cancer. Sci Rep 6:34234
Schaal, Jeffrey L; Li, Xinghai; Mastria, Eric et al. (2016) Injectable polypeptide micelles that form radiation crosslinked hydrogels in situ for intratumoral radiotherapy. J Control Release 228:58-66
Pilaz, Louis-Jan; Lennox, Ashley L; Rouanet, Jeremy P et al. (2016) Dynamic mRNA Transport and Local Translation in Radial Glial Progenitors of the Developing Brain. Curr Biol 26:3383-3392
Matak, Pavle; Matak, Andrija; Moustafa, Sarah et al. (2016) Disrupted iron homeostasis causes dopaminergic neurodegeneration in mice. Proc Natl Acad Sci U S A 113:3428-35
Friedman, Daphne R; Sibley, Alexander B; Owzar, Kouros et al. (2016) Relationship of blood monocytes with chronic lymphocytic leukemia aggressiveness and outcomes: a multi-institutional study. Am J Hematol 91:687-91

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