The School of Medicine, the Duke Comprehensive Cancer Institute, and the Institute for Genome Science & Policy have collaborated to create a Proteomics Core Facility to provide protein characterization resources for Duke Comprehensive Cancer Institute members and the entire Duke Research Gommunity. The Proteomics Core Facility ( is located in a ~1,900 sq. ft. laboratory in the Levine Science Research Center, and provides capabilities for mass spectrometry based proteomics for protein identification and protein quantitation, including biomarker discovery and biomarker verification experiments.'For qualitative identificafions and biomarker discovery experiments ('omic-scale qualitative and quantitative analyses), the laboratory is equipped with four high resolution accurate mass LC/MS/MS systems, each using a dedicated ultra-high performance nanoscale liquid chromatography systems (Waters NanoAcquity). Three ofthe MS/MS systems are hybrid quadruple time-of-fiight tandem mass spectrometers (Waters Q-Tof Ultima, Q-Tof Premier, and Synapt High Definition Mass Spectrometer). The fourth system is a hybrid linear ion trap - orbitrap system (Thermo's LTQ-Orbitrap). These systems can be used for label-free, gel-free'differential expression experiments, and also for isotope coding proteomics (such as SILAC or ITRAQ). For biomarker verification experiments, targeted mass spec quantitative experiments are performed on an ultra-high performance nanoscale LC system coupled to a triple quadrupole tandem mass spectrometer (Waters Quattro Premier). For these experiments, data acquisition is accomplished using LC/MS/MS with Mulfiple Reacfion Monitoring, a technology whiqh is the 'Gold Standard'for clinical pharmacokinetics studies. In addition, the Synapt High Definition: Mass Spectrometer (Q-Tof with an ion-mobility mass analyzer) provides for unique experiments characterizing piepfides and proteins not only on the basis of mass and charge, but also molecular shape/size, using a unique ionmobility cell located between the quadrupole and fime-of-fiight mass analyzers.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Duke University
United States
Zip Code
Galaz-Montoya, Monica; Wright, Sara J; Rodriguez, Gustavo J et al. (2017) ?2-Adrenergic receptor activation mobilizes intracellular calcium via a non-canonical cAMP-independent signaling pathway. J Biol Chem 292:9967-9974
Lanier, Megan L; Park, Hyeri; Mukherjee, Paramita et al. (2017) Formal Synthesis of (+)-Laurencin by Gold(I)-Catalyzed Intramolecular Dehydrative Alkoxylation. Chemistry 23:7180-7184
Shi, Qiong; Liu, Hongliang; Han, Peng et al. (2017) Genetic Variants in WNT2B and BTRC Predict Melanoma Survival. J Invest Dermatol 137:1749-1756
Woyach, Jennifer A; Ruppert, Amy S; Guinn, Daphne et al. (2017) BTKC481S-Mediated Resistance to Ibrutinib in Chronic Lymphocytic Leukemia. J Clin Oncol 35:1437-1443
Wang, Yanru; Freedman, Jennifer A; Liu, Hongliang et al. (2017) Associations between RNA splicing regulatory variants of stemness-related genes and racial disparities in susceptibility to prostate cancer. Int J Cancer 141:731-743
Fayanju, Oluwadamilola M; Hall, Carolyn S; Bauldry, Jessica Bowman et al. (2017) Body mass index mediates the prognostic significance of circulating tumor cells in inflammatory breast cancer. Am J Surg 214:666-671
Leu, David; Spasojevic, Ivan; Nguyen, Huy et al. (2017) CNS bioavailability and radiation protection of normal hippocampal neurogenesis by a lipophilic Mn porphyrin-based superoxide dismutase mimic, MnTnBuOE-2-PyP5. Redox Biol 12:864-871
Sauer, Scott J; Tarpley, Michael; Shah, Imran et al. (2017) Bisphenol A activates EGFR and ERK promoting proliferation, tumor spheroid formation and resistance to EGFR pathway inhibition in estrogen receptor-negative inflammatory breast cancer cells. Carcinogenesis 38:252-260
Pan, Yongchu; Liu, Hongliang; Wang, Yanru et al. (2017) Associations between genetic variants in mRNA splicing-related genes and risk of lung cancer: a pathway-based analysis from published GWASs. Sci Rep 7:44634
Yin, Jieyun; Liu, Hongliang; Liu, Zhensheng et al. (2017) Pathway-analysis of published genome-wide association studies of lung cancer: A potential role for the CYP4F3 locus. Mol Carcinog 56:1663-1672

Showing the most recent 10 out of 480 publications