Transgenic and genetically-engineered mutant animals serve as invaluable in vivo models for unraveling the molecular and genetic mechanisms leading to cancer and for evaluating new strategies for treating and preventing cancer. Efficient generation of transgenic and knockout animals requires great technical skill that is acquired only after extensive experience with animal husbandry and breeding, animal surgery, embryo manipulation, and micromanipulation. The Transgenic and Mutant Animal Shared Resource makes transgenic and knockout technologies accessible and affordable to the members of the University of Wisconsin Carbone Cancer Center (UWCCC). It uses the personnel, facilities and equipment from the campus-wide University of Wisconsin Biotechnology Center Transgenic Animal Facility (TAF). Services currently provided by the TAF include: ? Generation of transgenic mice and rats by pronuclear microinjection ? Cryopreservation of mouse and rat embryos ? Generation of knockout mice: generation of targeting vectors, generation of targeted embryonic stem (ES) cell clones, and generation of chimeric mice by injection of ES into host blastocyst ? Strain rederivations and ovarian transplants ? Other miscellaneous molecular biology and embryo services related to transgenic and knockout mouse production ? Consulting and training

Public Health Relevance

Genetically engineered mice and rats have proven to be invaluable models for understanding the molecular biology and molecular pathology of cancer. Through the Wisconsin Biotechnology Center Transgenic Animal Facility, UWCCC members have access to transgenic and mutant mouse models, including animal husbandry and breeding of strains, as well as scientific expertise, which allows investigators to perform cutting edge cancer research.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014520-40
Application #
8762772
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
40
Fiscal Year
2014
Total Cost
$122,746
Indirect Cost
$59,443
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Shiyanbola, Oyewale O; Sprague, Brian L; Hampton, John M et al. (2016) Emerging trends in surgical and adjuvant radiation therapies among women diagnosed with ductal carcinoma in situ. Cancer 122:2810-8
Valeri, Linda; Chen, Jarvis T; Garcia-Albeniz, Xabier et al. (2016) The Role of Stage at Diagnosis in Colorectal Cancer Black-White Survival Disparities: A Counterfactual Causal Inference Approach. Cancer Epidemiol Biomarkers Prev 25:83-9
Lu, Weifei; Hong, Hao; Cai, Weibo (2016) Radio-nanomaterials for biomedical applications: state of the art. Eur J Nanomed 8:151-170
Moore, Robert W; Fritz, Wayne A; Schneider, Andrew J et al. (2016) 2,3,7,8-Tetrachlorodibenzo-p-dioxin has both pro-carcinogenic and anti-carcinogenic effects on neuroendocrine prostate carcinoma formation in TRAMP mice. Toxicol Appl Pharmacol 305:242-9
Lor, Maichou; Xiong, Phia; Park, Linda et al. (2016) Western or Traditional Healers? Understanding Decision Making in the Hmong Population. West J Nurs Res :
Chang, Julie E; Havighurst, Thomas; Kim, KyungMann et al. (2016) Bendamustine + rituximab chemoimmunotherapy and maintenance lenalidomide in relapsed, refractory chronic lymphocytic leukaemia and small lymphocytic lymphoma: A Wisconsin Oncology Network Study. Br J Haematol 173:283-91
Guckenberger, David J; Pezzi, Hannah M; Regier, Mary C et al. (2016) Magnetic System for Automated Manipulation of Paramagnetic Particles. Anal Chem :
Hernandez, Reinier; Sun, Haiyan; England, Christopher G et al. (2016) CD146-targeted immunoPET and NIRF Imaging of Hepatocellular Carcinoma with a Dual-Labeled Monoclonal Antibody. Theranostics 6:1918-33
Damnernsawad, Alisa; Kong, Guangyao; Wen, Zhi et al. (2016) Kras is Required for Adult Hematopoiesis. Stem Cells 34:1859-71
Zhan, Yonghua; Ai, Fanrong; Chen, Feng et al. (2016) Intrinsically Zirconium-89 Labeled Gd2 O2 S:Eu Nanoprobes for In Vivo Positron Emission Tomography and Gamma-Ray-Induced Radioluminescence Imaging. Small 12:2872-6

Showing the most recent 10 out of 1196 publications