The Integrated Microscopy Facility (IMF) functions as a supervised, user-based Core providing state-of the- art microscopy imaging capabilities to UCCCC members and other University investigators. IMF staff additionally provide equipment maintenance, advice, and user training. Over half of the IMF usage is related to cancer research. Light microscopy offers one of the most flexible and informative tools available to researchers, providing scales of detail ranging from tens of millimeters to less than 10 nanometers. Available techniques provided by the IMF range from classic color histological stain imaging, to contrast generation in unstained cells, to fluorescence technologies. Capacity and capability have also increased annually by upgrades and purchases. Fluorescence methods continue to offer the greatest range of applications ranging from localization of multiple targets, to measurement of diverse biochemical or physiologic parameters in either fixed or living preparations. Accordingly, the greatest resources of the IMF are allocated to fluorescence microscopy. Over 100 peer-reviewed UCCCC investigators across the six Scientific Programs routinely used the IMF between 2008 and 2011, exceeding 50% of Facility usage in 2011. Overall use of the IMF continues to increase, presently exceeding 13,000 unit-hours annually (up from 9200h in 2007). Services provided by the IMF consist of the following: ? Provide high-end microscopy imaging capabilities, including brightfield (color, DIG, etc.), fluorescence (multi-dimension;TIRFM;bleaching, etc.), high-resolution, -speed, -sensitivity, confocal, and physiologic techniques. STED-CW super resolution microscopy is now available. ? Provide training on all instruments and expert advice to investigators regarding experiment design and execution. Provide oversight to assure proper equipment use and minimize breakage. ? Provide computers including networked data servers and software for image analysis (e.g., Huygens Professional deconvolution and Imaris packages) and image publication. ? Serve as an educational resource for University investigators in the use of current microscopy techniques and foster interactions among users. ? Serve as a demonstration site for new technologies.

Public Health Relevance

Microscopy provides a fundamental and increasingly-capable tool for basic and translational research. The IMF provides hands-on access to advanced equipment maintained by experts, as well as training, assistance, and advice.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Chicago
United States
Zip Code
Li, Gang; Montgomery, Jeffrey E; Eckert, Mark A et al. (2017) An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells. Nat Commun 8:1775
Stoddart, Angela; Wang, Jianghong; Hu, Chunmei et al. (2017) Inhibition of WNT signaling in the bone marrow niche prevents the development of MDS in the Apcdel/+ MDS mouse model. Blood 129:2959-2970
Wing, Claudia; Komatsu, Masaaki; Delaney, Shannon M et al. (2017) Application of stem cell derived neuronal cells to evaluate neurotoxic chemotherapy. Stem Cell Res 22:79-88
Shah, Palak; Trinh, Elaine; Qiang, Lei et al. (2017) Arsenic Induces p62 Expression to Form a Positive Feedback Loop with Nrf2 in Human Epidermal Keratinocytes: Implications for Preventing Arsenic-Induced Skin Cancer. Molecules 22:
Qiang, Lei; Sample, Ashley; Shea, Christopher R et al. (2017) Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis. Autophagy 13:2086-2103
Morita, Shuhei; Villalta, S Armando; Feldman, Hannah C et al. (2017) Targeting ABL-IRE1? Signaling Spares ER-Stressed Pancreatic ? Cells to Reverse Autoimmune Diabetes. Cell Metab 25:1207
Davis, Trevor L; Rebay, Ilaria (2017) Antagonistic regulation of the second mitotic wave by Eyes absent-Sine oculis and Combgap coordinates proliferation and specification in the Drosophila retina. Development 144:2640-2651
Kathayat, Rahul S; Elvira, Pablo D; Dickinson, Bryan C (2017) A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling. Nat Chem Biol 13:150-152
Hu, Xue; Li, Li; Yu, Xinyi et al. (2017) CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs). Oncotarget 8:111847-111865
Hasan, Yasmin; Waller, Joseph; Yao, Katharine et al. (2017) Utilization trend and regimens of hypofractionated whole breast radiation therapy in the United States. Breast Cancer Res Treat 162:317-328

Showing the most recent 10 out of 613 publications