The mission of the OSUCCC Proteomics Shared Resource (PSR) is to provide instrumentation, scientific expertise and shared research services for the identification of proteins, protein modifications and protein biomarkers to advance the productivity of cancer research of OSUCCC members at cost effective rates. High-quality proteomic analysis requires sophisticated protein separation approaches, image analysis capabilities, a variety of mass spectrometric systems, and advanced bioinformatics capabilities. The PSR is designed to provide affordable and high-quality service in each of these areas. Experienced PSR staff members provide effective services to identify proteins or protein complexes using the current state-of-theart mass spectrometers and supporting equipment Outstanding institutional support and value-added partnerships allow the PSR to offer a complete range of equipment and expertise for ID PAGE, 2D PAGE, multiplex analysis and differential image gel electrophoresis (DIGE), protein complex and post-translational modification analysis and other experimental approaches requiring protein identification. These services are linked to other key OSUCCC Shared Resources such as the Biomedical Informatics Shared Resource as well as PSR-supported bioinformatic tools required for data mining, including Mascot on a 16-node cluster. Kari Green-Church, Ph.D., and a team of highly-trained staff provide established scientific leadership for PSR to give OSUCCC cancer investigators effective technical expertise for proteomic analysis. Consultative services guide OSUCCC researchers seeking to address research questions from chemical and protein biomarker determination to unraveling complex protein-protein interactions from basic science experiments. Since 2004, over $4.0 million of institutional support has been invested in the operation and expansion of the PSR, providing staff salaries and cost matches for new instrumentation User fees generated are effectively leveraged for the operation costs (maintenance agreements, supplies and upgrades). The PSR has shown increased usage by OSUCCC members, now exceeding 48 members representing five of six OSUCCC Scientific Programs. OSUCCC members account for more than 56% of total usage of the PSR. This is a solid increase from 2004 when approximately 30 OSUCCC members used the shared resource and only constituted less than 30% of total usage. Collectively, PSR supports a wide range of services from standard proteomic assays to innovative consultative expertise to enhance cancer-relevant studies. It offers cancer investigators the critical tools and expertise needed for identifying proteins, quantifying protein expression levels, discovery of protein modifications and protein biomarkers We now request CCSG funding forthe PSR that will provide 19.4% of the total support for this important full Shared Resource over the next five years.

Public Health Relevance

The OSUCCC Proteomics Shared Resource (PSR) provides instrumentation, scientific expertise and shared research services needed for the identification of proteins, protein modifications and protein biomarkers to advance the quality of cancer research. The PSR is designed to provide affordable and high quality service in each of these areas, based on a cost-effective charge-back system. It provides a collaborative environment for researchers to discuss with PSR staff on how to solve research problems using cutting-edge technology, thereby enabling researchers to more effectively and efficiently solve [problems of chemical and protein biomarker and diagnosis on cancer samples and research projects.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Ohio State University
United States
Zip Code
Fazio, Nicola; Buzzoni, Roberto; Baudin, Eric et al. (2016) A Phase II Study of BEZ235 in Patients with Everolimus-resistant, Advanced Pancreatic Neuroendocrine Tumours. Anticancer Res 36:713-9
Eloy, Josimar O; Petrilli, Raquel; Topan, José Fernando et al. (2016) Co-loaded paclitaxel/rapamycin liposomes: Development, characterization and in vitro and in vivo evaluation for breast cancer therapy. Colloids Surf B Biointerfaces 141:74-82
Byrd, John C; Flynn, Joseph M; Kipps, Thomas J et al. (2016) Randomized phase 2 study of obinutuzumab monotherapy in symptomatic, previously untreated chronic lymphocytic leukemia. Blood 127:79-86
Datta, Jharna; Islam, Mozaffarul; Dutta, Samidha et al. (2016) Suberoylanilide hydroxamic acid inhibits growth of head and neck cancer cell lines by reactivation of tumor suppressor microRNAs. Oral Oncol 56:32-9
Wang, Hai; Agarwal, Pranay; Zhao, Shuting et al. (2016) Combined cancer therapy with hyaluronan-decorated fullerene-silica multifunctional nanoparticles to target cancer stem-like cells. Biomaterials 97:62-73
Villalona-Calero, Miguel A; Duan, Wenrui; Zhao, Weiqiang et al. (2016) Veliparib Alone or in Combination with Mitomycin C in Patients with Solid Tumors With Functional Deficiency in Homologous Recombination Repair. J Natl Cancer Inst 108:
Rai, K; Pilarski, R; Cebulla, C M et al. (2016) Comprehensive review of BAP1 tumor predisposition syndrome with report of two new cases. Clin Genet 89:285-94
Kerrigan, Kathleen; Shoben, Abigail; Otterson, Gregory (2016) Treatment of Lung Cancer Patients With Actionable Mutations in the Intensive Care Unit. Clin Lung Cancer 17:523-527
DiSilvestro, David J; Melgar-Bermudez, Emiliano; Yasmeen, Rumana et al. (2016) Leptin Production by Encapsulated Adipocytes Increases Brown Fat, Decreases Resistin, and Improves Glucose Intolerance in Obese Mice. PLoS One 11:e0153198
Terakawa, Jumpei; Rocchi, Altea; Serna, Vanida A et al. (2016) FGFR2IIIb-MAPK Activity Is Required for Epithelial Cell Fate Decision in the Lower Müllerian Duct. Mol Endocrinol 30:783-95

Showing the most recent 10 out of 1929 publications