The Flow Cytometry shared resource of the Virginia Commonwealth (VCU) Massey Cancer Center (MCC) was founded in 1976. Its instrumentation has been regularly upgraded through eight generations of flow cytometers to the five flow instruments currently in use, two multi-laser BD Aria II sorters, one of which is located in a Baker BioProtect Hood, three analyzers, a BD Canto II, a Coulter FC500 and a Coulter XL-MCL. Capabilities include up to nine color simultaneous detection and up to four simultaneous sorts per Aria II. The Flow Cytometry shared resource also has a Biacore T100 instrument for surface plasmon resonance (SPR) studies. The Director, Dr Daniel H. Conrad, has over 25 years of experience in flow cytometry and over 12 years of experience with SPR technology. Dr Conrad, whose office and laboratory are in close proximity to the instruments in the Kontos Medical Science Building (KMSB), meets regularly with core personnel and oversees planning for the new facility. The Resource Manager, Ms Julie Farnsworth, is highly trained in flow cytometry and has been with the Flow Cytometry shared resource for nine years. Previous to this, she worked for the Flow Cytometry core at the University of North Carolina (UNC) for two years. The resource provides a wide range of services, from routine fluorescence analysis to interactive custom design of innovative analysis and sorting protocols, addressing the specific needs of individual investigators. Dr Conrad closely follows the analyses performed by the core facility, insuring quality control, and advising facility users on alternative, often novel, approaches to experimental design and data analysis. Since the last review, the resource has upgraded facilities and instrumentation, moved to a new central location and added the capabilities for multiple high-speed and biohazardous sorting. This shared resource maintains a stable base of instrumentation, expertise and trained personnel and currently provides services and training for multiple users in 69 research groups from 21 departments at VCU. It has had a major impact on cancer research at the VCU MCC for 30 years.

Public Health Relevance

Flow cytometry has important applications in basic, translational, and clinical cancer research. Flow cytometry is used as a tool to measure molecules both on and in individual cells. This capacity gives cancer researchers the ability to identify the differences between normal and cancerous cells, to quantify the immune response to cancer cells, and to measure the response of cancer cells to treatment.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA016059-31
Application #
8299687
Study Section
Subcommittee G - Education (NCI)
Project Start
1978-12-01
Project End
2017-04-30
Budget Start
2012-08-30
Budget End
2013-04-30
Support Year
31
Fiscal Year
2012
Total Cost
$37,355
Indirect Cost
$12,780
Name
Virginia Commonwealth University
Department
Type
DUNS #
105300446
City
Richmond
State
VA
Country
United States
Zip Code
23298
Nulton, Tara J; Kim, Nak-Kyeong; DiNardo, Laurence J et al. (2018) Patients with integrated HPV16 in head and neck cancer show poor survival. Oral Oncol 80:52-55
Porter-Stransky, Kirsten A; Centanni, Samuel W; Karne, Saumya L et al. (2018) Noradrenergic Transmission at Alpha1-Adrenergic Receptors in the Ventral Periaqueductal Gray Modulates Arousal. Biol Psychiatry :
Cobb, Caroline O; Soule, Eric K; Rudy, Alyssa K et al. (2018) Patterns and Correlates of Tobacco and Cannabis co-use by Tobacco Product Type: Findings from the Virginia Youth Survey. Subst Use Misuse 53:2310-2319
Sonnenschein, Halie A; Lawrence, Kenneth F; Wittenberg, Karli A et al. (2018) Suppressor of IKKepsilon forms direct interactions with cytoskeletal proteins, tubulin and ?-actinin, linking innate immunity to the cytoskeleton. FEBS Open Bio 8:1064-1082
Curry, Zachary A; Wilkerson, Jenny L; Bagdas, Deniz et al. (2018) Monoacylglycerol Lipase Inhibitors Reverse Paclitaxel-Induced Nociceptive Behavior and Proinflammatory Markers in a Mouse Model of Chemotherapy-Induced Neuropathy. J Pharmacol Exp Ther 366:169-183
Radwa?ska, Malwina J; Jaskó?owski, Mateusz; Davydova, Elena et al. (2018) The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab. Acta Crystallogr D Struct Biol 74:681-689
Lancina 3rd, Michael G; Wang, Juan; Williamson, Geoffrey S et al. (2018) DenTimol as A Dendrimeric Timolol Analogue for Glaucoma Therapy: Synthesis and Preliminary Efficacy and Safety Assessment. Mol Pharm 15:2883-2889
Ginder, Gordon D; Williams Jr, David C (2018) Readers of DNA methylation, the MBD family as potential therapeutic targets. Pharmacol Ther 184:98-111
Chernoukhov, A; Hussein, A; Nkurunziza, S et al. (2018) Bayesian inference in time-varying additive hazards models with applications to disease mapping. Environmetrics 29:
Karandashova, Sophia; Kummarapurugu, Apparao B; Zheng, Shuo et al. (2018) Neutrophil elastase increases airway ceramide levels via upregulation of serine palmitoyltransferase. Am J Physiol Lung Cell Mol Physiol 314:L206-L214

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