The Biological Macromolecule Shared Resource (BMSR) represents a consolidation of services previously offered by the Molecular Biology and the Virus Vector shared resources, with the addition of a new service for recombinant protein production and purification. The overall goal of the BMSR is to facilitate and implement multiple end-points, both those within this shared resource, and those involving interfacing with other shared resources. These include the production of research grade virus particles, knock-in/knock-out mice (in collaboration with the Transgenic/Knockout Mouse Shared Resource), knock-in/knock-out somatic cell lines using homologous recombination, transient and stable protein expression in mammalian cells, recombinant protein production (bacterial, yeast, insect, and mammalian systems) and purification for structural analysis (in collaboration with the Structural Biology Shared Resource), and for protein interaction studies using the Biacore instrumentation located in the Flow Cytometry shared resource. In addition to moving vectors provided by investigators towards these end points, we offer design, construction and validation services for the generation of new vectors. The BMSR is co-directed by Dr Shirley Taylor (CMG), who brings molecular biology, cell culture, and protein expression experience, and Dr Darrell Peterson (Department of Biochemistry and Molecular Biology) a protein biochemist with extensive experience in protein production and purification. The BMSR employs a Resource Manager (Ms Kimberly Stratton, MS) and two full time research specialists, each of whom takes primary responsibility for one of the three major areas, but with sufficient cross-training in ail services offered to ensure continuity and maximal efficiency. The BMSR generally operates at >85% capacity, serving a wide range of investigators both within the Virginia Commonwealth University (VCU) Massey Cancer Center (MCC), and from the VCU research community. For the period January 1 2008 through December 31, 2010, MCC members with peer-reviewed funding accounted for approximately 70% of basic services and special projects. In CY 2010 the virus vector services of the BMSR served five funded MCC investigators, and three non-MCC investigators. Since its inception in January 2010, the Protein Production facility has served five funded MCC investigators and two non-MCC investigators, on ten separate projects. The BMSR served 22 peer-reviewed funded MCC investigators in routine services and special projects and 15 non-MCC investigators.

Public Health Relevance

The Biological Macromolecule Shared Resource assists MCC investigators in creating both DNA and protein molecules which in turn are used to study the molecular nature of cancer. Molecular manipulation provides the tools by which individual genes and proteins can be studied at the cellular and organismal levels. The production of purified protein allows investigators to determine the structure of cancer-related molecules, which in turn lends itself to understanding how the proteins can be manipulated to control cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA016059-33
Application #
8662707
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
33
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Virginia Commonwealth University
Department
Type
DUNS #
City
Richmond
State
VA
Country
United States
Zip Code
23298
Ma, Yibao; Min, Hae-Ki; Oh, Unsong et al. (2017) The lignan manassantin is a potent and specific inhibitor of mitochondrial complex I and bioenergetic activity in mammals. J Biol Chem 292:20989-20997
van der Weyden, Louise; Arends, Mark J; Campbell, Andrew D et al. (2017) Genome-wide in vivo screen identifies novel host regulators of metastatic colonization. Nature 541:233-236
Schurman, Lesley D; Smith, Terry L; Morales, Anthony J et al. (2017) Investigation of left and right lateral fluid percussion injury in C57BL6/J mice: In vivo functional consequences. Neurosci Lett 653:31-38
Newton, Jason; Hait, Nitai C; Maceyka, Michael et al. (2017) FTY720/fingolimod increases NPC1 and NPC2 expression and reduces cholesterol and sphingolipid accumulation in Niemann-Pick type C mutant fibroblasts. FASEB J 31:1719-1730
Manzanares, Miguel Á; Usui, Akihiro; Campbell, Deanna J et al. (2017) Transforming Growth Factors ? and ? Are Essential for Modeling Cholangiocarcinoma Desmoplasia and Progression in a Three-Dimensional Organotypic Culture Model. Am J Pathol 187:1068-1092
Arsenovic, Paul T; Mayer, Carl R; Conway, Daniel E (2017) SensorFRET: A Standardless Approach to Measuring Pixel-based Spectral Bleed-through and FRET Efficiency using Spectral Imaging. Sci Rep 7:15609
Lownik, Joseph C; Luker, Andrea J; Damle, Sheela R et al. (2017) ADAM10-Mediated ICOS Ligand Shedding on B Cells Is Necessary for Proper T Cell ICOS Regulation and T Follicular Helper Responses. J Immunol 199:2305-2315
Karki, Kishor; Saraiya, Siddharth; Hugo, Geoffrey D et al. (2017) Variabilities of Magnetic Resonance Imaging-, Computed Tomography-, and Positron Emission Tomography-Computed Tomography-Based Tumor and Lymph Node Delineations for Lung Cancer Radiation Therapy Planning. Int J Radiat Oncol Biol Phys 99:80-89
Meier, Jeremy A; Hyun, Moonjung; Cantwell, Marc et al. (2017) Stress-induced dynamic regulation of mitochondrial STAT3 and its association with cyclophilin D reduce mitochondrial ROS production. Sci Signal 10:
Benson, Zachary; Manjili, Saeed H; Habibi, Mehran et al. (2017) Conditioning neoadjuvant therapies for improved immunotherapy of cancer. Biochem Pharmacol 145:12-17

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