? Proteomics (PROT) Shared Resource The UNC Proteomics (PROT) Shared Resource (SR) provides state-of-the-art mass spectrometry-based qualitative and quantitative protein analyses for LCCC members. The PROT SR offers a variety of services to cancer researchers including: guidance in experimental design; protein and peptide identification; relative and absolute protein quantitation; analysis of protein modifications; activity-based proteomics; instrument training; and proteomic informatics. In addition to these basic proteomic services there are four cancer proteomic research themes, each involving active collaborations with LCCC investigators, which have been developed since the last renewal. These themes provide state-of-the art proteomic research technologies to members of the Cancer Center and include: i) chemical proteomics methods to interrogate the dynamic activation state of the cancer kinome; ii) quantifying both the proteome and phosphoproteome of patient tumors as part of the NCI CPTAC initiative; iii) mass spectrometry and computational methods for defining protein-protein interaction networks in cancer, including quantitative measurement of network dynamics; and iv) absolute quantification of proteins in complex protein mixtures by selective reaction monitoring (SRMs). The PROT SR is led by Lee Graves (MT), Professor of Pharmacology and director, and David Smalley, Facility Director. Three additional faculty are directly involved with the efforts of the PROT SR: Gary Johnson (MT), Kenan Distinguished Professor and Chair of the Department of Pharmacology and co-director of the Molecular Therapeutics Program, Ben Major (CCB), Associate Professor of Cell Biology & Physiology, and Xian Chen (IM), Professor of Biochemistry & Biophysics and technology director of the PROT SR. Together they provide strong synergistic leadership in mass spectrometry based proteomics with a proven record of productive collaboration with investigators in the Cancer Center.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee I - Career Development (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of North Carolina Chapel Hill
Chapel Hill
United States
Zip Code
Wang, Gary P; Terrault, Norah; Reeves, Jacqueline D et al. (2018) Prevalence and impact of baseline resistance-associated substitutions on the efficacy of ledipasvir/sofosbuvir or simeprevir/sofosbuvir against GT1 HCV infection. Sci Rep 8:3199
Phillips, Bonnie; Van Rompay, Koen K A; Rodriguez-Nieves, Jennifer et al. (2018) Adjuvant-Dependent Enhancement of HIV Env-Specific Antibody Responses in Infant Rhesus Macaques. J Virol 92:
Lianga, Noel; Doré, Carole; Kennedy, Erin K et al. (2018) Cdk1 phosphorylation of Esp1/Separase functions with PP2A and Slk19 to regulate pericentric Cohesin and anaphase onset. PLoS Genet 14:e1007029
Allott, Emma H; Geradts, Joseph; Cohen, Stephanie M et al. (2018) Frequency of breast cancer subtypes among African American women in the AMBER consortium. Breast Cancer Res 20:12
Dhungel, Bal Mukunda; Montgomery, Nathan D; Painschab, Matthew S et al. (2018) 'Discovering' primary effusion lymphoma in Malawi. AIDS 32:2264-2266
Cameron, Jennifer E; Rositch, Anne F; Vielot, Nadja A et al. (2018) Epstein-Barr Virus, High-Risk Human Papillomavirus and Abnormal Cervical Cytology in a Prospective Cohort of African Female Sex Workers. Sex Transm Dis 45:666-672
Lim, Joseph K; Liapakis, Ann Marie; Shiffman, Mitchell L et al. (2018) Safety and Effectiveness of Ledipasvir and Sofosbuvir, With or Without Ribavirin, in Treatment-Experienced Patients With Genotype 1 Hepatitis C Virus Infection and Cirrhosis. Clin Gastroenterol Hepatol 16:1811-1819.e4
Dronamraju, Raghuvar; Jha, Deepak Kumar; Eser, Umut et al. (2018) Set2 methyltransferase facilitates cell cycle progression by maintaining transcriptional fidelity. Nucleic Acids Res 46:1331-1344
Koehler, Jennifer W; Miller, Andrew D; Miller, C Ryan et al. (2018) A Revised Diagnostic Classification of Canine Glioma: Towards Validation of the Canine Glioma Patient as a Naturally Occurring Preclinical Model for Human Glioma. J Neuropathol Exp Neurol 77:1039-1054
Takaku, Motoki; Grimm, Sara A; Roberts, John D et al. (2018) GATA3 zinc finger 2 mutations reprogram the breast cancer transcriptional network. Nat Commun 9:1059

Showing the most recent 10 out of 1525 publications