The Transgenic and Chimeric Mouse Facility has been continuously funded by the NCI CCSG since 1989. Nancy Cooke, MD, Professor of Medicine and Genetics, has provided leadership to this Shared Resource since 1993. Dr. Cooke is an experienced investigator with considerable expertise in transgenic and gene knock-out experimentation. Co-Facility Director Stephen Liebhaber, MD, Professor of Genetics and Medicine, has worked with Dr. Cooke since the Facility was first planned and has contributed significantly to the initial establishment and subsequent expansion of many of its services. An experienced technical team under Jean Richa, PhD, provides expertise in a range of transgenic technologies, enabling the Facility to regularly introduce new and improved services. Among the services provided by the Transgenic and Chimeric Mouse Facility are: generation of transgenic mice via microinjections of DNA into fertilized oocyte; generation of chimeric mice via injection of embryonic stem cells into blastocysts;embryo rederivation; embryo and sperm cryopreservation;in vitro fertilization;centralized and monitored cryopreservation storage;and maintenance of useful animal stocks including recombination-activation gene expression lines. The Core has intracytoplasmic sperm injection capability to complement the in vitro fertilization service. Newly developed services during the current funding period were computer generated logs for ordering, online tracking of services for investigators, acquisition of a centralized and dedicated cryopreservation facility with on-line catalog and tracking of lines under cryostorage, genotyping of tail DNAs, and integration of mouse ES microinjection service with centralized gene knock-out services. Over 20 ACC members have used the facility in the last year. ACC member usage is 41% of the total core usage. CCSG support represents 21% of the proposed core budget with the remaining funding coming from charge backs and other grants/contracts. Additional institutional support is provided in the form of funding for equipment maintenance and the new cryopreservation storage facility.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA016520-38
Application #
8593271
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
38
Fiscal Year
2014
Total Cost
$197,172
Indirect Cost
$86,401
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Taylor, Laura A; Abraham, Ronnie M; Tahirovic, Emin et al. (2017) High ALDH1 expression correlates with better prognosis in tumorigenic malignant melanoma. Mod Pathol 30:634-639
Rebecca, Vito W; Nicastri, Michael C; McLaughlin, Noel et al. (2017) A Unified Approach to Targeting the Lysosome's Degradative and Growth Signaling Roles. Cancer Discov 7:1266-1283
Till, Jacob E; Yoon, Changhwan; Kim, Bang-Jin et al. (2017) Oncogenic KRAS and p53 Loss Drive Gastric Tumorigenesis in Mice That Can Be Attenuated by E-Cadherin Expression. Cancer Res 77:5349-5359
Ewens, Kathryn G; Bhatti, Tricia R; Moran, Kimberly A et al. (2017) Phosphorylation of pRb: mechanism for RB pathway inactivation in MYCN-amplified retinoblastoma. Cancer Med 6:619-630
Chee, Wonshik; Lee, Yaelim; Im, Eun-Ok et al. (2017) A culturally tailored Internet cancer support group for Asian American breast cancer survivors: A randomized controlled pilot intervention study. J Telemed Telecare 23:618-626
Zang, Tianzhu; Taplin, Mary-Ellen; Tamae, Daniel et al. (2017) Testicular vs adrenal sources of hydroxy-androgens in prostate cancer. Endocr Relat Cancer 24:393-404
Walter, David M; Venancio, Olivia S; Buza, Elizabeth L et al. (2017) Systematic In Vivo Inactivation of Chromatin-Regulating Enzymes Identifies Setd2 as a Potent Tumor Suppressor in Lung Adenocarcinoma. Cancer Res 77:1719-1729
Carrer, Alessandro; Parris, Joshua L D; Trefely, Sophie et al. (2017) Impact of a High-fat Diet on Tissue Acyl-CoA and Histone Acetylation Levels. J Biol Chem 292:3312-3322
Fennelly, Colin; Amaravadi, Ravi K (2017) Lysosomal Biology in Cancer. Methods Mol Biol 1594:293-308
Safo, Sandra E; Li, Shuzhao; Long, Qi (2017) Integrative analysis of transcriptomic and metabolomic data via sparse canonical correlation analysis with incorporation of biological information. Biometrics :

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