The purpose of the Microscopy, Imaging and Cytometry Resources (MICR) Core is to enhance the peer reviewed funded research activities of KCI members whose research requires confocal microscopy cytometry, or small animal imaging related techniques. Capabilities and services include: confocal microscopy, multiphoton microscopy, high resolution conventional light microscopy, FRET and FRAP, in vivo small animal imaging (including MR, PET and optical imaging), flow cytometry, complete animal and cellular histology services (including cytochemistry, histochemistry, immunocytochemistry immunohistochemistry) and ratiometric analyses (e.g., intracellular pH and ion measurement studies), as well as three and four dimensional image reconstruction and quantitative measurements. Our advanced imaging systems consist of a Zeiss LSM 310 confocal microscope equipped with four laser lines, the newly acquired PerkinElmer UltraView ERS spinning disk confocal microscope equipped with three laser lines, a Zeiss LSM 510 META NLO confocal microscope with eight laser lines and multiphoton imaging capability, the newly acquired Leica TCS SPS MP equipped with nine laser lines and multiphoton capability. Our flow cytometry systems include LSR II, FACSCanto, FACSCalibur and FACScan flow cytometers as well as the FACSDiVa cell sorter. The facility also provides conventional fluorescent microscopy through a Zeiss Axiophot Triple-Camera Photomicroscope, a Zeiss live cell imaging microscope with the state-of-the-art Apotome module capable of four dimensional imaging (3D in time). In vivo small animal imaging is a newly acquired combination of technologies made available to the core through the addition of a Kodak IS4000MM small animal optical/X-Ray imager, Bruker 4.7T and 11.7T small animal MR imagers and a Concord MicroPET small animal PET scanner. The analytical systems of the MICR include five Apple and PC workstations with various analytical software and photographic quality printers as well as the MIP (Molecular Imaging Portal), a UNIX based central server with 45 tb storage capacity and a 74 tb robotic tape backup system as well as a dedicated applications server hosting the advance image analysis software, Matlab? and Definiens?, with five licenses each allowing up to five users access to each software simultaneously. Since the last review in 2004, there has been a substantial investment in the core for new equipment and upgrades at a cost exceeding $2,800,000. All of the funding was from sources other than the CCSG.

Public Health Relevance

MICR is a service oriented support facility that provides advanced imaging and cytometry research instrumentation, technologies and expertise to a number of KCI members'peer reviewed research projects that are directly related to human health at all levels of basic science, translational and clinical research areas.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA022453-30
Application #
8380969
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-01-09
Budget End
2012-11-30
Support Year
30
Fiscal Year
2012
Total Cost
$231,810
Indirect Cost
$79,303
Name
Wayne State University
Department
Type
DUNS #
001962224
City
Detroit
State
MI
Country
United States
Zip Code
48202
Kraniak, Janice M; Chalasani, Anita; Wallace, Margaret R et al. (2018) Development of 3D culture models of plexiform neurofibroma and initial application for phenotypic characterization and drug screening. Exp Neurol 299:289-298
An, Myunggi; Yu, Chunsong; Xi, Jingchao et al. (2018) Induction of necrotic cell death and activation of STING in the tumor microenvironment via cationic silica nanoparticles leading to enhanced antitumor immunity. Nanoscale 10:9311-9319
Neslund-Dudas, Christine M; McBride, Russell B; Kandegedara, Ashoka et al. (2018) Association between cadmium and androgen receptor protein expression differs in prostate tumors of African American and European American men. J Trace Elem Med Biol 48:233-238
Wu, Jheng-Yu; Xiang, Shengyan; Zhang, Mu et al. (2018) Histone deacetylase 6 (HDAC6) deacetylates extracellular signal-regulated kinase 1 (ERK1) and thereby stimulates ERK1 activity. J Biol Chem 293:1976-1993
Negmeldin, Ahmed T; Knoff, Joseph R; Pflum, Mary Kay H (2018) The structural requirements of histone deacetylase inhibitors: C4-modified SAHA analogs display dual HDAC6/HDAC8 selectivity. Eur J Med Chem 143:1790-1806
Tamura, Koji; Yu, Jun; Hata, Tatsuo et al. (2018) Mutations in the pancreatic secretory enzymes CPA1 and CPB1 are associated with pancreatic cancer. Proc Natl Acad Sci U S A 115:4767-4772
Matherly, Larry H; Hou, Zhanjun; Gangjee, Aleem (2018) The promise and challenges of exploiting the proton-coupled folate transporter for selective therapeutic targeting of cancer. Cancer Chemother Pharmacol 81:1-15
Pollack, Murray M; Holubkov, Richard; Reeder, Ron et al. (2018) PICU Length of Stay: Factors Associated With Bed Utilization and Development of a Benchmarking Model. Pediatr Crit Care Med 19:196-203
Bao, Xun; Wu, Jianmei; Sanai, Nader et al. (2018) A liquid chromatography with tandem mass spectrometry method for quantitating total and unbound ceritinib in patient plasma and brain tumor. J Pharm Anal 8:20-26
Heath, Elisabeth I; Lynce, Filipa; Xiu, Joanne et al. (2018) Racial Disparities in the Molecular Landscape of Cancer. Anticancer Res 38:2235-2240

Showing the most recent 10 out of 826 publications