9.1.5 FLOW CYTOMETRY SHARED SERVICE The Flow Cytometry Shared Service (FCSS) has served investigators of the Arizona Cancer Center (AZCC) for over 20 years. We offer AZCC investigators efficient and reliable flow cytometric services with high standards of quality control and productivity. We provide the knowledge and assistance necessary to design experiments, develop new assays, perform flow cytometric functions and interpret data. Our service supports the developrnent of new and innovative basic and clinicalstudies in cancer prevention, carcinogenesis, and cell and niolecular biology, and laboratory-based pre-clinical and translational drug development studies of new cancer therapies utilizing flow cytometric techniques. Flow cytometry instrumentation comprises a FACScan, FACSAria and LSR II, along wth associated software packages for data analysis and presentation. A number of methodologies are offered: cell sorting, cell cycle/ploidy analysis, multi^fluorochrome immunophenotyping, apoptosis/necrosis analysis, multicytokine detection in supernatants/plasma, intracellular antigen measurement, and intracelluar pH/calcium analysis. Personnel include the Director, Dr. Amanda Baker, and two expert cytometrists, one of whom also serves as facility manager. The FCSS has experienced continuous growth from 2003 thru 2008, both with regard to the service itself and investigator usage. In 2004 the FCSS completed a merger with the University Arizona Research Laboratories (ARL) Cytometry Core Service. By combining administration, expertise and equipment, a premier FCSS was created for our AZCC investigators. Usage activity by AZCC program investigators has increased three-fold with the number of AZCC members using the facility increasing approximately two-fold (from 20 in 2003 to 38 in 2008). During this time period, a total of 48 peer-reviewed publications have been produced by AZCC investigators using the FCSS. Overall, the FCSS operates efficiently and effectively for our AZCC investigators with continued growth and usage.

Public Health Relevance

The Flow Cytometry Shared Service offers an affordable resource for expert assistance in experimental design, execution, and analysis for Conducting studies utilizing the latest technology in flow cytometery.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023074-35
Application #
8540940
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
35
Fiscal Year
2013
Total Cost
$92,706
Indirect Cost
$31,873
Name
University of Arizona
Department
Type
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Bea, Jennifer W; Jurutka, Peter W; Hibler, Elizabeth A et al. (2015) Concentrations of the vitamin D metabolite 1,25(OH)2D and odds of metabolic syndrome and its components. Metabolism 64:447-59
Leung, Sarah J; Rice, Photini S; Barton, Jennifer K (2015) In vivo molecular mapping of the tumor microenvironment in an azoxymethane-treated mouse model of colon carcinogenesis. Lasers Surg Med 47:40-9
Dickinson, Sally E; Rusche, Jadrian J; Bec, Sergiu L et al. (2015) The effect of sulforaphane on histone deacetylase activity in keratinocytes: Differences between in vitro and in vivo analyses. Mol Carcinog 54:1513-20
Landowski, Terry H; Gard, Jaime; Pond, Erika et al. (2014) Targeting integrin ?6 stimulates curative-type bone metastasis lesions in a xenograft model. Mol Cancer Ther 13:1558-66
Quintero-Ramos, A; GutiƩrrez-Rubio, S A; Del Toro-Arreola, A et al. (2014) Association between polymorphisms in the thymidylate synthase gene and risk of breast cancer in a Mexican population. Genet Mol Res 13:8749-56
Gustafson, Heather L; Yao, Song; Goldman, Bryan H et al. (2014) Genetic polymorphisms in oxidative stress-related genes are associated with outcomes following treatment for aggressive B-cell non-Hodgkin lymphoma. Am J Hematol 89:639-45
Jiang, Yanlong; Kong, Qingke; Roland, Kenneth L et al. (2014) Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity. Int J Med Microbiol 304:431-43
Chow, H-H Sherry; Garland, Linda L; Heckman-Stoddard, Brandy M et al. (2014) A pilot clinical study of resveratrol in postmenopausal women with high body mass index: effects on systemic sex steroid hormones. J Transl Med 12:223
Kang, Hyun-Jin; Kendrick, Samantha; Hecht, Sidney M et al. (2014) The transcriptional complex between the BCL2 i-motif and hnRNP LL is a molecular switch for control of gene expression that can be modulated by small molecules. J Am Chem Soc 136:4172-85
Kurzius-Spencer, Margaret; Burgess, Jefferey L; Harris, Robin B et al. (2014) Contribution of diet to aggregate arsenic exposures-an analysis across populations. J Expo Sci Environ Epidemiol 24:156-62

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