9.1.5 FLOW CYTOMETRY SHARED SERVICE The Flow Cytometry Shared Service (FCSS) has served investigators of the Arizona Cancer Center (AZCC) for over 20 years. We offer AZCC investigators efficient and reliable flow cytometric services with high standards of quality control and productivity. We provide the knowledge and assistance necessary to design experiments, develop new assays, perform flow cytometric functions and interpret data. Our service supports the developrnent of new and innovative basic and clinicalstudies in cancer prevention, carcinogenesis, and cell and niolecular biology, and laboratory-based pre-clinical and translational drug development studies of new cancer therapies utilizing flow cytometric techniques. Flow cytometry instrumentation comprises a FACScan, FACSAria and LSR II, along wth associated software packages for data analysis and presentation. A number of methodologies are offered: cell sorting, cell cycle/ploidy analysis, multi^fluorochrome immunophenotyping, apoptosis/necrosis analysis, multicytokine detection in supernatants/plasma, intracellular antigen measurement, and intracelluar pH/calcium analysis. Personnel include the Director, Dr. Amanda Baker, and two expert cytometrists, one of whom also serves as facility manager. The FCSS has experienced continuous growth from 2003 thru 2008, both with regard to the service itself and investigator usage. In 2004 the FCSS completed a merger with the University Arizona Research Laboratories (ARL) Cytometry Core Service. By combining administration, expertise and equipment, a premier FCSS was created for our AZCC investigators. Usage activity by AZCC program investigators has increased three-fold with the number of AZCC members using the facility increasing approximately two-fold (from 20 in 2003 to 38 in 2008). During this time period, a total of 48 peer-reviewed publications have been produced by AZCC investigators using the FCSS. Overall, the FCSS operates efficiently and effectively for our AZCC investigators with continued growth and usage.

Public Health Relevance

The Flow Cytometry Shared Service offers an affordable resource for expert assistance in experimental design, execution, and analysis for Conducting studies utilizing the latest technology in flow cytometery.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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University of Arizona
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Bea, Jennifer W; Jurutka, Peter W; Hibler, Elizabeth A et al. (2015) Concentrations of the vitamin D metabolite 1,25(OH)2D and odds of metabolic syndrome and its components. Metabolism 64:447-59
Leung, Sarah J; Rice, Photini S; Barton, Jennifer K (2015) In vivo molecular mapping of the tumor microenvironment in an azoxymethane-treated mouse model of colon carcinogenesis. Lasers Surg Med 47:40-9
Dickinson, Sally E; Rusche, Jadrian J; Bec, Sergiu L et al. (2015) The effect of sulforaphane on histone deacetylase activity in keratinocytes: Differences between in vitro and in vivo analyses. Mol Carcinog 54:1513-20
Quintero-Ramos, A; GutiƩrrez-Rubio, S A; Del Toro-Arreola, A et al. (2014) Association between polymorphisms in the thymidylate synthase gene and risk of breast cancer in a Mexican population. Genet Mol Res 13:8749-56
Landowski, Terry H; Gard, Jaime; Pond, Erika et al. (2014) Targeting integrin ?6 stimulates curative-type bone metastasis lesions in a xenograft model. Mol Cancer Ther 13:1558-66
Jiang, Yanlong; Kong, Qingke; Roland, Kenneth L et al. (2014) Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity. Int J Med Microbiol 304:431-43
Gustafson, Heather L; Yao, Song; Goldman, Bryan H et al. (2014) Genetic polymorphisms in oxidative stress-related genes are associated with outcomes following treatment for aggressive B-cell non-Hodgkin lymphoma. Am J Hematol 89:639-45
Kang, Hyun-Jin; Kendrick, Samantha; Hecht, Sidney M et al. (2014) The transcriptional complex between the BCL2 i-motif and hnRNP LL is a molecular switch for control of gene expression that can be modulated by small molecules. J Am Chem Soc 136:4172-85
Chow, H-H Sherry; Garland, Linda L; Heckman-Stoddard, Brandy M et al. (2014) A pilot clinical study of resveratrol in postmenopausal women with high body mass index: effects on systemic sex steroid hormones. J Transl Med 12:223
Lancaster, Jordan J; Juneman, Elizabeth; Arnce, Sarah A et al. (2014) An electrically coupled tissue-engineered cardiomyocyte scaffold improves cardiac function in rats with chronic heart failure. J Heart Lung Transplant 33:438-45

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