Genomics Shared Resource (GSR) The goal of the Genomics Shared Resource (GSR) is to provide advanced genomics technology and services required to support the full range of cancer research conducted at the University of Arizona Cancer Center (UACC). Single gene to full genome analyses is one cornerstone of cancer biology research and discovery. The GSR provides UACC Members with guided, high-quality, cost-efficient access to a full spectrum of genomic cornerstone technologies. The GSR delivers the following services: 1) next-generation sequencing; 2) epigenetic analysis; 3) gene expression profiling; 4) genome-wide DNA analysis; and 5) genomic DNA variance. Within these categories of service, techniques include whole genome expression profiling, comparative genomic hybridization, sequence-based copy number variance, whole exome sequencing, shotgun sequencing, targeted sequencing panels, RNA-seq, chromatin immunoprecipitation (ChIP)-seq, genotyping, epigenomic ChIP-chip assays, real-time quantitative PCR (qPCR), sample quality assurance/quality control (QA / QC), sample isolation, experimental consultation, data analysis, and data archiving. With the ability to analyze single gene/small gene sets as well as genome wide analyses, the GSR offers great flexibility in analytical approaches. In addition, the GSR provides the capability for developing custom applications and makes new technologies available to address the specific needs of UACC investigators. GSR equipment includes: two Ion Torrent Personal Genome Machines, an Ion Torrent Proton (for small to large next-generation sequencing needs), Affymetrix and Agilent microarray platforms (for hybridization-based services), and three Applied Biosystems qPCR machines (for full service and self-service PCR applications). The GSR provides consultation on experiment design, and sample preparation, followed by sample QA / QC in preparation for the analysis. Data analysis and storage are critical to investigator's ability to access their data for publication and dissemination. Accordingly, the GSR provides microarray, sequence, and qPCR analysis to support the preparation of publications and grants. In addition, the Service provides access to raw data through a password protected website, along with triply redundant backup of investigator's data for five years.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023074-37
Application #
9315734
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2017-07-01
Budget End
2018-06-30
Support Year
37
Fiscal Year
2017
Total Cost
Indirect Cost
Name
University of Arizona
Department
Type
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Mushtaq, Adeela; Kapoor, Vikas; Latif, Azka et al. (2018) Efficacy and toxicity profile of carfilzomib based regimens for treatment of multiple myeloma: A systematic review. Crit Rev Oncol Hematol 125:1-11
Rak, Michael A; Buehler, Jason; Zeltzer, Sebastian et al. (2018) Human Cytomegalovirus UL135 Interacts with Host Adaptor Proteins To Regulate Epidermal Growth Factor Receptor and Reactivation from Latency. J Virol 92:
Rojo de la Vega, Montserrat; Zhang, Donna D; Wondrak, Georg T (2018) Topical Bixin Confers NRF2-Dependent Protection Against Photodamage and Hair Graying in Mouse Skin. Front Pharmacol 9:287
Bea, Jennifer W; de Heer, Hendrik Dirk; Valdez, Luis et al. (2018) Physical Activity among Navajo Cancer Survivors: A Qualitative Study. Am Indian Alsk Native Ment Health Res 25:54-73
Siyahian, Aida; Malik, Saad Ullah; Mushtaq, Adeela et al. (2018) Prophylaxis for Hepatitis B Virus Reactivation after Allogeneic Stem Cell Transplantation in the Era of Drug Resistance and Newer Antivirals: A Systematic Review and Meta-Analysis. Biol Blood Marrow Transplant 24:1483-1489
Stanton, Annette L; Wiley, Joshua F; Krull, Jennifer L et al. (2018) Cancer-related coping processes as predictors of depressive symptoms, trajectories, and episodes. J Consult Clin Psychol 86:820-830
Sinharay, Sanhita; Randtke, Edward A; Howison, Christine M et al. (2018) Detection of Enzyme Activity and Inhibition during Studies in Solution, In Vitro and In Vivo with CatalyCEST MRI. Mol Imaging Biol 20:240-248
Sun, Virginia; Crane, Tracy E; Slack, Samantha D et al. (2018) Rationale, development, and design of the Altering Intake, Managing Symptoms (AIMS) dietary intervention for bowel dysfunction in rectal cancer survivors. Contemp Clin Trials 68:61-66
Agasid, Mark T; Wang, Xuemin; Huang, Yiding et al. (2018) Expression, purification, and electrophysiological characterization of a recombinant, fluorescent Kir6.2 in mammalian cells. Protein Expr Purif 146:61-68
Wales, Jessica A; Chen, Cheng-Yu; Breci, Linda et al. (2018) Discovery of stimulator binding to a conserved pocket in the heme domain of soluble guanylyl cyclase. J Biol Chem 293:1850-1864

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