The availability of the Transgenic Mouse Shared Resource enables our CCSG Research Base Investigators to conduct versatile, cutting-edge research with a battery of sophisticated genetic techniques for manipulation of the mouse genome to create models for studies of cancer for incisive in vivo mechanistic investigations of the fundamental processes involved in the etiology of tumor development. Transgenic mice carrying new or novel genes are created by microinjection of DNA into the pronuclei of fertilized eggs and """"""""knock-out"""""""" mice lacking specific genes of interest are created by homologous recombination in embryonic stem cells followed by injection into blastocysts to create chimeric mice for breeding to homozygosity. The high degree of conservation of most sequences in genomes of humans and mice makes using mouse genetic manipulation technology to create models of human cancer pathogenesis extremely useful. These approaches are remarkably powerful in cancer research particularly in the analysis of oncogenes, metastasis, cell-cycle control, tumor suppressor genes, and in the crafting of cancer model systems for developing new treatment regimens, and methods for drug testing and tumor imaging. The mission of this Shared Resource is to provide the highly technical aspects of manipulation of genes in embryos and mouse embryonic stem cells as a service to our Center members, allowing them to create the genetically manipulated mouse models they need. This is an outstanding example of specialized techniques, highly trained dedicated personnel, and expensive equipment can be accessed by researchers who could not reasonably expect to develop or obtain them on an individual basis. The UCSD CCSG Shared Resource provides cutting-edge, rapidly evolving services that adapt and drive the field of mouse genetics and continue to be very responsive to the vast creativity of the CCSG membership, fulfilling their needs for the most cutting-edge embryology and molecular genetics in the mouse.

Public Health Relevance

The availability of the Transgenic Mouse shared Resource enables our researchers to conduct versatile cutting-edge research with a battery of sophisticated genetic techniques to address the fundamental questions in cancer research. Mouse models are ideal for determining the genetic basis of oncogenesis and studying the fundamental processes involved in the etiology of tumor development and metastasis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA023100-28
Application #
8934858
Study Section
Subcommittee G - Education (NCI)
Program Officer
Shafik, Hasnaa
Project Start
2014-07-21
Project End
2019-04-30
Budget Start
2014-07-21
Budget End
2015-04-30
Support Year
28
Fiscal Year
2014
Total Cost
$300,282
Indirect Cost
$104,623
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Singh, Siddharth; Loomba, Rohit (2018) Role of two-dimensional shear wave elastography in the assessment of chronic liver diseases. Hepatology 67:13-15
Hartman, Sheri J; Nelson, Sandahl H; Myers, Emily et al. (2018) Randomized controlled trial of increasing physical activity on objectively measured and self-reported cognitive functioning among breast cancer survivors: The memory & motion study. Cancer 124:192-202
Hoffmann, Hanne M; Gong, Ping; Tamrazian, Anika et al. (2018) Transcriptional interaction between cFOS and the homeodomain-binding transcription factor VAX1 on the GnRH promoter controls Gnrh1 expression levels in a GnRH neuron maturation specific manner. Mol Cell Endocrinol 461:143-154
Liu, Xuxiang; Cao, Minghui; Palomares, Melanie et al. (2018) Metastatic breast cancer cells overexpress and secrete miR-218 to regulate type I collagen deposition by osteoblasts. Breast Cancer Res 20:127
Huang, Justin K; Carlin, Daniel E; Yu, Michael Ku et al. (2018) Systematic Evaluation of Molecular Networks for Discovery of Disease Genes. Cell Syst 6:484-495.e5
Kalyanaraman, Hema; Schwaerzer, Gerburg; Ramdani, Ghania et al. (2018) Protein Kinase G Activation Reverses Oxidative Stress and Restores Osteoblast Function and Bone Formation in Male Mice With Type 1 Diabetes. Diabetes 67:607-623
Hartman, Sheri J; Marinac, Catherine R; Cadmus-Bertram, Lisa et al. (2018) Sedentary Behaviors and Biomarkers Among Breast Cancer Survivors. J Phys Act Health 15:1-6
Wu, Yan; Tamayo, Pablo; Zhang, Kun (2018) Visualizing and Interpreting Single-Cell Gene Expression Datasets with Similarity Weighted Nonnegative Embedding. Cell Syst 7:656-666.e4
Dow, Michelle; Pyke, Rachel M; Tsui, Brian Y et al. (2018) Integrative genomic analysis of mouse and human hepatocellular carcinoma. Proc Natl Acad Sci U S A 115:E9879-E9888
Que, Xuchu; Hung, Ming-Yow; Yeang, Calvin et al. (2018) Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. Nature 558:301-306

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